2020
DOI: 10.1002/ajpa.24039
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Identifying new lineages in the Y chromosome of Colombian Amazon indigenous populations

Abstract: Objectives: The Y chromosome has highly informative markers, such as singlenucleotide polymorphisms (SNPs), that are useful for making historical inferences about the settlement of the Americas. However, the scarcity of these markers has limited their use. This study aims to identify new SNPs and increase the phylogenetic resolution of haplogroup Q for the Americas, mainly focusing on the lineages of the Amazon region. Materials and Methods: Next-generation sequencing was performed on two Y chromosomes belongi… Show more

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Cited by 5 publications
(12 citation statements)
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References 33 publications
(67 reference statements)
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“…In a previous study by Rivera et al [21], the complete genome of two indigenous samples from the Colombian Amazon was sequenced. From the results of these sequences, only the Y chromosome data were filtered and used to search for new variants of phylogenetic importance.…”
Section: Next Generation Sequencing (Ngs) and Search For Variants Of ...mentioning
confidence: 99%
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“…In a previous study by Rivera et al [21], the complete genome of two indigenous samples from the Colombian Amazon was sequenced. From the results of these sequences, only the Y chromosome data were filtered and used to search for new variants of phylogenetic importance.…”
Section: Next Generation Sequencing (Ngs) and Search For Variants Of ...mentioning
confidence: 99%
“…The 24 selected variants were typed by allele-specific PCR in all samples from the communities studied (Tucano, Pasto, Nasa, Embera katio, Kogu ¨i and Arhuaco). In addition to the samples from the Colombian Caribbean (Arhuaco and Kogu ¨i), the 14 SNPs reported by Rivera et al [21] were typified. The SNPs CO17, CO25, CO26 and Z5915 were typed individually, and the PCR reaction was performed under the following conditions 1X buffer, 4.00mM MgCl2, 0.025mM dNTPs, 0.32μM primers, 0.017U/μL Taq polymerase and 10 ng DNA in a 15μL reaction.…”
Section: Snp Genotyping and Haplogroup Classificationmentioning
confidence: 99%
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