Identifying key genes associated with acute myocardial infarction

Abstract

Background:This study aimed to identify key genes associated with acute myocardial infarction (AMI) by reanalyzing microarray data.Methods:Three gene expression profile datasets GSE66360, GSE34198, and GSE48060 were downloaded from GEO database. After data preprocessing, genes without heterogeneity across different platforms were subjected to differential expression analysis between the AMI group and the control group using metaDE package. P < .05 was used as the cutoff for a differentially expressed gene (DEG). The expression data matrices of DEGs were imported in ReactomeFIViz to construct a gene functional interaction (FI) network. Then, DEGs in each module were subjected to pathway enrichment analysis using DAVID. MiRNAs and transcription factors predicted to regulate target DEGs were identified. Quantitative real-time polymerase chain reaction (RT-PCR) was applied to verify the expression of genes.Result:A total of 913 upregulated genes and 1060 downregulated genes were identified in the AMI group. A FI network consists of 21 modules and DEGs in 12 modules were significantly enriched in pathways. The transcription factor-miRNA-gene network contains 2 transcription factors FOXO3 and MYBL2, and 2 miRNAs hsa-miR-21-5p and hsa-miR-30c-5p. RT-PCR validations showed that expression levels of FOXO3 and MYBL2 were significantly increased in AMI, and expression levels of hsa-miR-21–5p and hsa-miR-30c-5p were obviously decreased in AMI.Conclusion:A total of 41 DEGs, such as SOCS3, VAPA, and COL5A2, are speculated to have roles in the pathogenesis of AMI; 2 transcription factors FOXO3 and MYBL2, and 2 miRNAs hsa-miR-21-5p and hsa-miR-30c-5p may be involved in the regulation of the expression of these DEGs.

In Silico Analysis of Differential Gene Expression in Three Common Rat Models of Diastolic Dysfunction

“…VIP (vasoactive intestinal peptide), cardioprotective in heart failure ( 88 ), and SMAD5 , which has anti-apoptotic actions in cardiac myocytes ( 89 ) were upregulated. On the other hand, AKAP12 , which may protect against IR injury ( 90 ), was downregulated, as were both MYBL2 and TUBB2A , which were recently shown to be upregulated in the heart after acute MI ( 91 , 92 ).…”

MicroRNAs: New Biomarkers for the progression of Coronary Artery Diseases

“…In terms of the cardiovascular diseases, several molecules have already been used as biomarkers, such as the low-E3S Web of Conferences 185, 03039 (2020) ICEEB 2020 http://doi.org/10.1051/e3sconf/202018503039 density lipoprotein cholesterol, circulating brain natriuretic peptide (BNP) and so on [24] . Unfortunately, few of these biomarkers have been currently transformed into practical diagnostic applications [25,26] . With the development of precision medicine and next generation sequencing, miRNAs have shown great advantages in serving as diagnostic and prognostic biomarkers for CADs due to its high specificity, sensitivity and convenient availability [27] .…”

Collapsing the list of myocardial infarction-related differentially expressed genes into a diagnostic signature

“…Among non-coding RNAs, miRNAs are of particular interest in the context of robustness of biological processes, since they regulate key elements of extensive segments of signaling pathways' networks [8][9][10]. To date, consistency of expressional changes of miRNAs and their target genes has been investigated in macrophages of pigs and rats with experimental MI [11] and in the whole blood of MI patients [12].…”