Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study

Quadros, Maurício de; Bugs, Roberto Carlos Freitas; Soares, Renata de Oliveira; Rossato, Adriana Medianeira; Rocha, Lisiane da Luz; d’Azevedo, Pedro Alves

doi:10.1016/j.abd.2019.11.004

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Previous studies conducted by Quadros et al established that Staphylococcus epidermidis, micrococcus species, and corynebacterium species were frequently identified on dermatoscope lenses and smartphone adaptors. 2 Presence of human papilloma virus DNA has also been reported in a study on dermoscopic lenses. The aerosol particle sizes of COVID-19 range from 3 to 100 nm and using an filtering face piece 3 respirator offers a filtration rate of 99% for all particles measuring up to 0.6 μm.…”

mentioning

confidence: 77%

Performing dermoscopy in the COVID ‐19 pandemic

Goldust

Zalaudek

Gupta³

et al. 2020

Dermatologic Therapy

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mentioning

confidence: 77%

Performing dermoscopy in the COVID ‐19 pandemic

Goldust

Zalaudek

Gupta³

et al. 2020

Dermatologic Therapy

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“…In contrast, the combination of whole genome sequencing technology and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) detection technology could discover as many drug resistance mechanisms as possible and rapidly screen for sensitive drugs [ 19 , 20 , 21 , 22 ]. As a valuable means of studying the mechanisms of bacterial drug resistance, MALDI-TOF MS is only applicable to relatively few bacteria, based on the principle of bacterial drug resistance [ 23 ]. Therefore, it is urgent to seek a rapid, efficient detection platform of bacterial resistance.…”

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confidence: 99%

Microfluidic Chip for Detection of Drug Resistance at the Single-cell Level

Song

et al. 2022

Micromachines

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Drug-resistant bacterial strains seriously threaten human health. Rapid screening of antibiotics is urgently required to improve clinical treatment. Conventional methods of antimicrobial susceptibility testing rely on turbidimetry that is evident only after several days of incubation. The lengthy time of the assay can delay clinical treatment. Here, we proposed a single-cell level rapid system based on a microfluidic chip. The detection period of 30 min to 2 h was significantly shorter than the conventional turbidity-based method. To promote detection efficiency, 16 independent channels were designed, permitting the simultaneous screening of 16 drugs in the microfluidic chip. Prepositioning of drugs in the chip permitted prolonged transportation and storage. This may allow for the widespread use of the novel system, particularly in the regions where medical facilities are scarce. The growth curves were reported rapidly through a custom code in Matlab after tracking and photographing the bacteria during microscopy examination. The capability of the proposed system was validated by antimicrobial susceptibility testing trials with standard strains. The system provides a potentially useful detection tool for drug-resistant bacteria.

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Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study

Cited by 2 publications

References 27 publications

Performing dermoscopy in the COVID ‐19 pandemic

Performing dermoscopy in the COVID ‐19 pandemic

Microfluidic Chip for Detection of Drug Resistance at the Single-cell Level

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