Identifying gene regulatory modules of heat shock response in yeast

Wu, Weisheng; Li, Wen-Hsiung

doi:10.1186/1471-2164-9-439

Cited by 47 publications

(40 citation statements)

References 39 publications

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“…4). Interestingly, Cst6 belongs to five novel heat-responsive TFs recently identified using a gene regulatory module which was constructed based on TF-promoter binding matrix and heat shock gene expression data (63). In addition, Cst6 is reported to regulate genes involved in the cell cycle and DNA processing (63).…”

Section: Differential Proteomic Patterns Of Industrial Strains In Tr mentioning

confidence: 99%

“…Interestingly, Cst6 belongs to five novel heat-responsive TFs recently identified using a gene regulatory module which was constructed based on TF-promoter binding matrix and heat shock gene expression data (63). In addition, Cst6 is reported to regulate genes involved in the cell cycle and DNA processing (63). Therefore, much more significant correlation of Cst6 to the proteomic variation observed in TR than in HSR implied a potential crosstalk between cell cycle modulation and thermotolerant adaptation.…”

Section: Differential Proteomic Patterns Of Industrial Strains In Tr mentioning

confidence: 99%

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Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae

Wang

Xiong

Xiao

et al. 2015

Molecular & Cellular Proteomics

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Saccharomyces cerevisiae has been intensively studied in responses to different environmental stresses such as heat shock through global omic analysis. However, the S. cerevisiae industrial strains with superior thermotolerance have not been explored in any proteomic studies for elucidating the tolerance mechanism. Recently a new diploid strain was obtained through evolutionary engineering of a parental industrial strain, and it exhibited even higher resistance to prolonged thermal stress. Herein, we performed iTRAQ-based quantitative proteomic analysis on both the parental and evolved industrial strains to further understand the mechanism of thermotolerant adaptation. Out of ϳ2600 quantifiable proteins from biological quadruplicates, 193 and 204 proteins were differentially regulated in the parental and evolved strains respectively during heat-stressed growth. The proteomic response of the industrial strains cultivated under prolonged thermal stress turned out to be substantially different from that of the laboratory strain exposed to sudden heat shock. Further analysis of transcription factors underlying the proteomic perturbation also indicated the distinct regulatory mechanism of thermotolerance. Finally, a cochaperone Mdj1 and a metabolic enzyme Adh1 were selected to investigate their roles in mediating heatstressed growth and ethanol production of yeasts. Our proteomic characterization of the industrial strain led to comprehensive understanding of the molecular basis of thermotolerance, which would facilitate future improvement in the industrially important trait of S. cerevisiae by rational engineering. Molecular & Cellular Proteomics

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Section: Differential Proteomic Patterns Of Industrial Strains In Tr mentioning

confidence: 99%

Section: Differential Proteomic Patterns Of Industrial Strains In Tr mentioning

confidence: 99%

Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae

Wang

Xiong

Xiao

et al. 2015

Molecular & Cellular Proteomics

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“…As expected, most of the analyses have shown that heat shock proteins are very important for the thermotolerance, but in addition, several proteins involved in oxidative stress response, signal transduction, transcription, translation, and carbohydrate or nitrogen metabolism were observed (1,19,40). In S. cerevisiae, the heat shock response is mostly controlled by Hsf1, Msn2/Msn4, and Hac1, and targets for these transcription factors have already been identified (22,27,(63)(64)(65)(66). Upon heat shock, the A. fumigatus Hsf1 homologue has increased transcription (1,22).…”

Section: Discussionmentioning

confidence: 59%

Molecular Characterization of the Putative Transcription Factor SebA Involved in Virulence in Aspergillus fumigatus

et al. 2012

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ABSTRACTAspergillus fumigatusis a major opportunistic pathogen and allergen of mammals. Nutrient sensing and acquisition mechanisms, as well as the capability to cope with different stressing conditions, are essential forA. fumigatusvirulence and survival in the mammalian host. This study characterized theA. fumigatusSebA transcription factor, which is the putative homologue of the factor encoded byTrichoderma atrovirideseb1. The ΔsebAmutant demonstrated reduced growth in the presence of paraquat, hydrogen peroxide, CaCl2, and poor nutritional conditions, while viability associated withsebAwas also affected by heat shock exposure. Accordingly, SebA::GFP (SebA::green fluorescent protein) was shown to accumulate in the nucleus upon exposure to oxidative stress and heat shock conditions. In addition, genes involved in either the oxidative stress or heat shock response had reduced transcription in the ΔsebAmutant. TheA. fumigatusΔsebAstrain was attenuated in virulence in a murine model of invasive pulmonary aspergillosis. Furthermore, killing of the ΔsebAmutant by murine alveolar macrophages was increased compared to killing of the wild-type strain.A. fumigatusSebA plays a complex role, contributing to several stress tolerance pathways and growth under poor nutritional conditions, and seems to be integrated into different stress responses.

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“…In this regard, a TF is considered as a cell cycle TF if a statistically significant portion of its regulatory targets is the cell cycle-regulated genes (identified by Spellman et al (1998)). The hypergeometric distribution is used to test the statistical significance (Wu and Li, 2008a). The procedure for determining whether TFj is a cell cycle TF is as follows.…”

Section: Identification Of Cell Cycle Tfsmentioning

confidence: 99%

Yeast cell cycle transcription factors identification by variable selection criteria

Wang¹,

Wang²,

Wu³

2011

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Identifying gene regulatory modules of heat shock response in yeast

Cited by 47 publications

References 39 publications

Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae

Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae

Molecular Characterization of the Putative Transcription Factor SebA Involved in Virulence in Aspergillus fumigatus

Yeast cell cycle transcription factors identification by variable selection criteria

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