Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

Abstract

Retinal neovascularization is a complication which caused human vision loss severely. It has been shown that circular RNAs (circRNAs) play essential roles in gene regulation. However, circRNA expression profile and the underlying mechanisms in retinal neovascular diseases remain unclear. In the present study, we identified altered circRNAs in the retinas of oxygen-induced retinopathy (OIR) mouse model by microarray profiling. Microarray analysis revealed that 539 circRNAs were significantly altered in OIR retinas compared with controls. Among them, 185 up-regulated and 354 down-regulated circRNAs were identified. The expression levels of 4 altered circRNAs including mmu_circRNA_002573, mmu_circRNA_011180, mmu_circRNA_016108 and mmu_circRNA_22546 were validated by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Bioinformatic analysis with validated circRNAs such as competing endogenous RNA (ceRNA) regulatory networks with Gene Ontology (GO) enrichment analysis demonstrated that qRT-PCR validated circRNAs were associated with cellular process, cell part and phosphoric ester hydrolase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that MAPK signaling pathway and renin-angiotensin system were related to validated circRNAs, suggesting these pathways may participate in pathological angiogenesis. The results together suggested that circRNAs were aberrantly expressed in OIR retinas and may play potential roles in retinal neovascular diseases.

Comprehensive evaluation of differential long non‑coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra

“…Although the normalized data were skewed, the distributions of the log2 ratios among the six samples presented an excellent similarity after normalization, which indicated that the data could be further analyzed. The phenomenon that the normalized data were skewed (long tail above mean/median) was also demonstrated in previously published studies (58)(59)(60). It was suggested that the sampling error and selective bias are responsible for this phenomenon, and enlarging the sample sizes is an effective strategy to decrease the sampling error and selective bias.…”

A novel circular RNA hsa_circRNA_103809/miR-377-3p/GOT1 pathway regulates cisplatin-resistance in non-small cell lung cancer (NSCLC)

“…Emerging evidences indicated that circRNAs sponged miRNAs to exert their biological functions [19][20][21], and hsa_circRNA_103809 facilitated cancer progressions by regulating miRNAs in a competing endogenous RNA (ceRNA)-dependent manner [14,[16][17][18]22]. By searching the online Pubmed database (https://pubmed.ncbi.nlm.nih.gov/), the miRNAs, including miR-101-3p [17], miR-532-3p [14], miR-4302 [18], miR-620 [22] and miR-377-3p [16], could be sponged by hsa_circRNA_103809.…”

“…According to our recent advances in circRNAs research, circRNAs exerted their biological functions and regulated genes expression through serving as RNA sponges to competitively bind to microRNAs (miRNAs) [19][20][21]. Taken hsa_circRNA_103809 as an example, previous publications indicated that hsa_circRNA_103809 sponged multiple RNAs, including miR-101-3p [17], miR-532-3p [14], miR-4302 [18], miR-620 [22] and miR-377-3p [16].…”

Emerging roles of non‐coding RNAs in retinal diseases: A review

“…A total of 539 circRNAs were significantly altered, and 4 altered circRNAs, mmu_circRNA_002573, mmu_ circRNA_011180, mmu_circRNA_016108 and mmu_ circRNA_22546, were validated by qRT-PCR. 123…”

“…In addition, circRNAs were detected by microarray assay in the retinas of an OIR mouse model. A total of 539 circRNAs were significantly altered, and 4 altered circRNAs, mmu_circRNA_002573 , mmu_circRNA_011180 , mmu_circRNA_016108 and mmu_circRNA_22546 , were validated by qRT‐PCR 123 …”