Identifying changes in the wheat kernel proteome under heat stress using iTRAQ

Zhang, Yufeng; Lou, Hongyao; Guo, Dandan; Zhang, Ruiqi; Su, Meng; Hou, Zhenghong; Zhou, Haiying; Liang, Rongqi; Xie, Chaojie; You, Minsheng; Li, Baoyun

doi:10.1016/j.cj.2018.04.003

Cited by 27 publications

(18 citation statements)

References 46 publications

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“…However, kernel length was less affected by the stresses than kernel weight and other related traits ( Table 1 ). This finding is in agreement with a report that kernel length is less affected by heat than the kernel weight [ 33 ]. We identified some MSD lines, such as MSD187, that could maintain good kernel weight under stress, unlike Norin 61 and Imam.…”

Section: Discussionsupporting

confidence: 94%

Exploitation of Tolerance of Wheat Kernel Weight and Shape-Related Traits from Aegilops tauschii under Heat and Combined Heat-Drought Stresses

Elhadi

Kamal

Gorafi

et al. 2021

IJMS

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Kernel weight and shape-related traits are inherited stably and increase wheat yield. Narrow genetic diversity limits the progress of wheat breeding. Here, we evaluated kernel weight and shape-related traits and applied genome-wide association analysis to a panel of wheat multiple synthetic derivative (MSD) lines. The MSD lines harbored genomic fragments from Aegilops tauschii. These materials were grown under optimum conditions in Japan, as well as under heat and combined heat–drought conditions in Sudan. We aimed to explore useful QTLs for kernel weight and shape-related traits under stress conditions. These can be useful for enhancing yield under stress conditions. MSD lines possessed remarkable genetic variation for all traits under all conditions, and some lines showed better performance than the background parent Norin 61. We identified 82 marker trait associations (MTAs) under the three conditions; most of them originated from the D genome. All of the favorable alleles originated from Ae. tauschii. For the first time, we identified markers on chromosome 5D associated with a candidate gene encoding a RING-type E3 ubiquitin–protein ligase and expected to have a role in regulating wheat seed size. Our study provides important knowledge for the improvement of wheat yield under optimum and stress conditions. The results emphasize the importance of Ae. tauschii as a gene reservoir for wheat breeding.

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Section: Discussionsupporting

confidence: 94%

Exploitation of Tolerance of Wheat Kernel Weight and Shape-Related Traits from Aegilops tauschii under Heat and Combined Heat-Drought Stresses

Elhadi

Kamal

Gorafi

et al. 2021

IJMS

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“…The optimum lettuce growth temperature is between 15 and 20˚C; temperatures above 30˚C have resulted in early lettuce bolting [5], and early bolting decreases both quality and production. In our study, we used isobaric tags for relative and absolute quantitation (iTRAQ) to distinguish differentially expressed proteins [6,7].…”

Section: Introductionmentioning

confidence: 99%

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature

Qin

Shaochuan³

et al. 2020

PLoS ONE

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High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located on 1,766 phosphopeptides from 987 phosphoproteins were identified after high-temperature treatment,among which 217 phosphoproteins significantly changed their expression abundance (116 upregulated and 101 downregulated). Most phosphoproteins for which the abundance was altered were associated with the metabolic process, with the main molecular functions were catalytic activity and transporter activity. Regarding the functional pathway, starch and sucrose metabolism was the mainly enriched signaling pathways. Hence, high temperature influenced phosphoprotein activity, especially that associated with starch and sucrose metabolism. We suspected that the lettuce shorten its growth cycle and reduce vegetative growth owing to changes in the contents of starch and soluble sugar after high temperature stress, which then led to early bolting/flowering. These findings improve our understanding of the regulatory molecular mechanisms involved in lettuce bolting.

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“…However, the identification of 2-DE is limited by protein abundance (proteins with low abundance are unable to be detected), narrow pI range coverage [17]. On the other hand, isobaric tags for the relative and absolute quantitation (iTRAQ) technique is a high-throughput proteomic technique with higher sensitivity that allows simultaneous identification and quantification of proteins, including low-abundant proteins, in no more than eight samples with high coverage [18,19]. The technique has been applied to Arabidopsis thaliana [20], Zea mays L. [21], Brassica napus [22,23], Triticum aestivum L. [24] and Nicotiana tabacum [25] in recent years.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Quantitative Analysis of Responsive Proteins Under PEG-Induced Drought Stress in Wheat Leaves

Wang

Zhang

Huang

et al. 2019

IJMS

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Drought is an important abiotic stress that seriously restricts crop productivity. An understanding of drought tolerance mechanisms offers guidance for cultivar improvement. In order to understand how a well-known wheat genotype Jinmai 47 responds to drought, we adopted the iTRAQ and LC/MS approaches and conducted proteomics analysis of leaves after exposure to 20% of polyethylene glycol-6000 (PEG)-induced stress for 4 days. The study identified 176 differentially expressed proteins (DEPs), with 65 (36.5%) of them being up-regulated, and 111 (63.5%) down-regulated. DEPs, located in cellular membranes and cytosol mainly, were involved in stress and redox regulation (51), carbohydrate and energy metabolism (36), amino acid metabolism (24), and biosynthesis of other secondary metabolites (20) primarily. Under drought stress, TCA cycle related proteins were up-regulated. Antioxidant system, signaling system, and nucleic acid metabolism etc. were relatively weakened. In comparison, the metabolism pathways that function in plasma dehydration protection and protein structure protection were strongly enhanced, as indicated by the improved biosynthesis of 2 osmolytes, sucrose and Proline, and strongly up-regulated protective proteins, LEA proteins and chaperones. SUS4, P5CSs, OAT, Rab protein, and Lea14-A were considered to be important candidate proteins, which deserve to be further investigated.

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Identifying changes in the wheat kernel proteome under heat stress using iTRAQ

Cited by 27 publications

References 46 publications

Exploitation of Tolerance of Wheat Kernel Weight and Shape-Related Traits from Aegilops tauschii under Heat and Combined Heat-Drought Stresses

Exploitation of Tolerance of Wheat Kernel Weight and Shape-Related Traits from Aegilops tauschii under Heat and Combined Heat-Drought Stresses

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature

iTRAQ-Based Quantitative Analysis of Responsive Proteins Under PEG-Induced Drought Stress in Wheat Leaves

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