Identifying cell and molecular stress after radiation in a three-dimensional (3-D) model of oral mucositis

Lambros, Maria P.; Parsa, Cyrus; Mulamalla, HariChandana; Orlando, Robert; Lau, Bernard; Huang, Ying; Pon, Doreen; Chow, Moses S. S.

doi:10.1016/j.bbrc.2010.12.135

Cited by 25 publications

(22 citation statements)

References 22 publications

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“…Previous research on this protein in the eye showed an up-regulation following an inflammation-associated corneal neovascularization (88). S100-a13 has also been shown to play a role in damage-associated molecular patterns, which interact with the NF-B pathway and various inflammatory cytokines such as IL-1B and IL-8, (92) which implicates this important pathway in our model.…”

Section: Resultsmentioning

confidence: 88%

Proteomics Analyses of Human Optic Nerve Head Astrocytes Following Biomechanical Strain

Rogers

Dharsee²,

Ackloo³

et al. 2012

Molecular & Cellular Proteomics

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We investigate the role of glial cell activation in the human optic nerve caused by raised intraocular pressure, and their potential role in the development of glaucomatous optic neuropathy. To do this we present a proteomics study of the response of cultured, optic nerve head astrocytes to biomechanical strain, the magnitude and mode of strain based on previously published quantitative models. In this case, astrocytes were subjected to 3 and 12% stretches for either 2 h or 24 h. Proteomic methods included nano-liquid chromatography, tandem mass spectrometry, and iTRAQ labeling. Using controls for both stretch and time, a six-plex iTRAQ liquid chromatography-tandem MS (LC/MS/MS) experiment yielded 573 proteins discovered at a 95% confidence limit. The pathways included transforming growth factor ␤1, tumor necrosis factor, caspase 3, and tumor protein p53, which have all been implicated in the activation of astrocytes and are believed to play a role in the development of glaucomatous optic neuropathy.

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Section: Resultsmentioning

confidence: 88%

Proteomics Analyses of Human Optic Nerve Head Astrocytes Following Biomechanical Strain

Rogers

Dharsee²,

Ackloo³

et al. 2012

Molecular & Cellular Proteomics

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“…Radiation injuries differ from the above in that perfusion is initially uninterrupted, but cell survival is limited by the balance between DNA damage repair pathways, ROS responses, and cell death pathways that are triggered by radiation-induced DNA double strand breaks. Damage-associated molecular patterns (DAMPs) expressed on irradiated cells can also trigger an inflammatory response by the innate immune system that can compromise tissue survival [6]. …”

Section: Introductionmentioning

confidence: 99%

Therapeutic Targeting of CD47 to Modulate Tissue Responses to Ischemia and Radiation

Pantoja¹

2011

J Genet Syndr Gene Ther

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“…Mannose-grafted polymeric NPs, for example, showed high accumulation on a Caco-2 pro-inflammatory cytokine model (Coco et al, 2013). 3D in vitro cellular models of oral and intestinal inflammation may also prove useful (Frontela-Saseta et al 2013;Lambros et al, 2011). One such model uses Caco-2 cells co-cultured with primary macrophages and dendritic cells derived from human blood, and then treated with interleukin 1-beta to induce inflammation (Leonard et al, 2010).…”

Section: Models Of Inflammationmentioning

confidence: 99%

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Venema²,

et al. 2014

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Engineered metal/mineral, lipid and biochemical macromolecule nanomaterials (NMs) have potential applications in food. Methodologies for the assessment of NM digestion and bioavailability in the gastrointestinal tract are nascent and require refinement. A working group was tasked by the International Life Sciences Institute NanoRelease Food Additive project to review existing models of the gastrointestinal tract in health and disease, and the utility of these models for the assessment of the uptake of NMs intended for food. Gastrointestinal digestion and absorption could be addressed in a tiered approach using in silico computational models, in vitro non-cellular fluid systems and in vitro cell culture models, after which the necessity of ex vivo organ culture and in vivo animal studies can be considered. Examples of NM quantification in gastrointestinal tract fluids and tissues are emerging; however, few standardized analytical techniques are available. Coupling of these techniques to gastrointestinal models, along with further standardization, will further strengthen methodologies for risk assessment.

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Identifying cell and molecular stress after radiation in a three-dimensional (3-D) model of oral mucositis

Cited by 25 publications

References 22 publications

Proteomics Analyses of Human Optic Nerve Head Astrocytes Following Biomechanical Strain

Proteomics Analyses of Human Optic Nerve Head Astrocytes Following Biomechanical Strain

Therapeutic Targeting of CD47 to Modulate Tissue Responses to Ischemia and Radiation

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

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