Identifying Antimicrobial Multiresistance Patterns of<i>Escherichia coli</i>Sampled From Diarrhoeic Calves by Cluster Analysis Techniques: A Way to Guide Research on Multiresistance Mechanisms

Botrel, Marie-Anne; Morignat, Eric; Meunier, Danièle; Madec, Jean‐Yves; Calavas, Didier

doi:10.1111/j.1863-2378.2009.01262.x

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Agglomerative Hierarchical Clustering (HC) was used, which is based on a dissimilarity matrix and has the advantage of not having the number of clusters chosen a priori [ 26 ]. The Gower distance was used to calculate the distance matrix, and Ward's method was used as the HC algorithm [ 27 ]. The functions ‘hclust’ and ‘daisy’ from the package ‘cluster’ in R were used to conduct the HC and the Gower distance respectively [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance in wildlife and in the built environment in a wildlife rehabilitation center

et al. 2021

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Injured and orphaned wildlife are often brought to Wildlife Rehabilitation Centers (WRC) to be cared for by professionals to ultimately be released back to their natural habitats. In these centers, animals may spend months and frequently receive prolonged antibiotic therapy. Therefore, WRC may play a role in the emergence and dissemination of antimicrobial resistance (AMR). The goal of this study was to investigate the presence and antibiotic resistance profiles of Gram-negative bacteria with reduced susceptibility to cephalosporins in both the wildlife admitted to a WRC and in the WRC built environment in Chile. A cross-sectional study was conducted sampling animals undergoing rehabilitation ( n = 64) and the WRC environment ( n = 160). Isolated bacterial species were identified with MALDI-TOF, and antimicrobial susceptibility determined using the disk diffusion method. Enterobacteriaceae and Pseudomonadaceae were the dominant bacterial families among the environmental ( n = 78) and animal ( n = 31) isolates. For Enterobacteriaceae , isolates of the most abundant species ( E. coli ) were classified into 20 antibiotic resistance profiles, with eight of those isolates being resistant to more than nine antibiotics, including imipenem. Isolates of the Pseudomonadaceae family identified 11 isolates with resistance to antibiotics such as carbapenems and quinolones. Even though a cluster analysis based on antibiotic resistance patterns did not show a clear overlap between environmental and animal isolates, it is important to highlight the identification of isolates resistant to carbapenems, which is very relevant from a public health perspective. Further, numerous antibiotic resistance profiles were observed in different bacterial species, indicating not only environmental contamination with a wide diversity of bacteria, but also a wide diversity of resistant bacteria in animals at the WRC. The approach taken by sampling animals and their hospital environment can be useful in understanding AMR dynamics in wildlife rehabilitation settings, as well as the potential dissemination of AMR into the natural environment.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance in wildlife and in the built environment in a wildlife rehabilitation center

et al. 2021

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“…on the R plasmid. 10,11,21,31 Chloramphenicol resistance was associated with the use of phenicols. The use of chloramphenicol in production animals was banned in 1998 in Japan.…”

Section: Direct Selection and Cross Selectionmentioning

confidence: 98%

Multivariable Analysis of the Association Between Antimicrobial Use and Antimicrobial Resistance inEscherichia coliIsolated from Apparently Healthy Pigs in Japan

Makita

Goto

Ozawa

et al. 2016

Microbial Drug Resistance

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The objective of this study was to investigate the association between antimicrobial agent use and antimicrobial resistance in Escherichia coli isolated from healthy pigs using data from 2004 to 2007 in the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM). Fecal E. coli isolates from 250 pigs (one isolate each from a pig per farm) were examined for antimicrobial resistance. Information on the use of antimicrobials within preceding 6 months and types of farms recorded in JVARM was collected and statistically analyzed against the resistance patterns. In the univariate analysis, associations between both therapeutic and feed additive use of antimicrobials, and resistance to dihydrostreptomycin, gentamicin, kanamycin, ampicillin, cefazolin, ceftiofur, oxytetracycline, chloramphenicol, trimethoprim, nalidixic acid, enrofloxacin, colistin, and bicozamycin, and husbandry factors were investigated. In multivariable analysis, generalized estimating equations were used to control geographical intraclass correlation. Confounding for structurally unrelated associations was tested using generalized linear models. The results suggested direct and cross selections in the associations between use of aminoglycosides in reproduction farms and resistance to kanamycin, use of tetracyclines in larger farms and resistance to oxytetracycline, use of beta-lactams and resistance to ampicillin, use of phenicols and resistance to chloramphenicol, and use of fluoroquinolones and resistance to nalidixic acid and enrofloxacin. Coselection was suggested in the use of tetracyclines and chloramphenicol resistance. The associations between use of beta-lactams and dihydrostreptomycin resistance, use of macrolides and ampicillin and oxytetracycline resistance, and use of colistin and kanamycin resistance were significant, but were confounded by the simultaneous use of homologous antimicrobials.

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Modification of outer membrane permeability and alteration of LPS in veterinary enterotoxigenic Escherichia coli

Davin-Régli

Guérin-Faublée

Pagès

2019

Research in Veterinary Science

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Identifying Antimicrobial Multiresistance Patterns ofEscherichia coliSampled From Diarrhoeic Calves by Cluster Analysis Techniques: A Way to Guide Research on Multiresistance Mechanisms

Cited by 5 publications

References 19 publications

Antimicrobial resistance in wildlife and in the built environment in a wildlife rehabilitation center

Antimicrobial resistance in wildlife and in the built environment in a wildlife rehabilitation center

Multivariable Analysis of the Association Between Antimicrobial Use and Antimicrobial Resistance inEscherichia coliIsolated from Apparently Healthy Pigs in Japan

Modification of outer membrane permeability and alteration of LPS in veterinary enterotoxigenic Escherichia coli

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