Identifying a potential receptor for the antibacterial peptide of sponge Axinella donnani endosymbiont

Vimala, A.; Gromiha, M. Michael

doi:10.1016/j.gene.2015.04.070

Cited by 3 publications

(2 citation statements)

References 50 publications

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“…Since Rhodophyte and Glaucophyte cTPs start with a conserved phenylalanine [ 92 ], chloroplast protein import could have benefited from a functional inversion of this cyanobacterial protein in the evolution of the TOC complex. Although this observation was taken as an argument against the emergence of cTPs from HA-RAMPs [ 93 ], we argue that HA-RAMPs would have originally interacted with the outer membrane lipid headgroups [ 5 ], then crossed the outer membrane spontaneously [ 94 , 95 ], with some HA-RAMPs also interacting with Omp85 proteins [ 96 ]. In this view, the most likely evolutionary scenario for chloroplast import has involved recruitment of Omp85 to improve delivery of HA-RAMP-tagged proteins to the import-and-destroy receptor at the inner membrane surface.…”

Section: Discussionmentioning

confidence: 99%

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Garrido

Caspari

Choquet

et al. 2020

Cells

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Mitochondria and chloroplasts emerged from primary endosymbiosis. Most proteins of the endosymbiont were subsequently expressed in the nucleo-cytosol of the host and organelle-targeted via the acquisition of N-terminal presequences, whose evolutionary origin remains enigmatic. Using a quantitative assessment of their physico-chemical properties, we show that organelle targeting peptides, which are distinct from signal peptides targeting other subcellular compartments, group with a subset of antimicrobial peptides. We demonstrate that extant antimicrobial peptides target a fluorescent reporter to either the mitochondria or the chloroplast in the green alga Chlamydomonas reinhardtii and, conversely, that extant targeting peptides still display antimicrobial activity. Thus, we provide strong computational and functional evidence for an evolutionary link between organelle-targeting and antimicrobial peptides. Our results support the view that resistance of bacterial progenitors of organelles to the attack of host antimicrobial peptides has been instrumental in eukaryogenesis and in the emergence of photosynthetic eukaryotes.

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Section: Discussionmentioning

confidence: 99%

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Garrido

Caspari

Choquet

et al. 2020

Cells

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“…Another BAM-targeted peptide that has promise as a putative therapeutic comes from the marine sponge Axinella donnani [ 76 ]. Using a comparative genomics approach verified with substrate and docking analysis, it was elucidated that the receptor for this peptide was Omp85 (BamA).…”

Section: Targeting Bam With Peptides and Proteinsmentioning

confidence: 99%

Targeting BAM for Novel Therapeutics against Pathogenic Gram-Negative Bacteria

et al. 2023

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The growing emergence of multidrug resistance in bacterial pathogens is an immediate threat to human health worldwide. Unfortunately, there has not been a matching increase in the discovery of new antibiotics to combat this alarming trend. Novel contemporary approaches aimed at antibiotic discovery against Gram-negative bacterial pathogens have expanded focus to also include essential surface-exposed receptors and protein complexes, which have classically been targeted for vaccine development. One surface-exposed protein complex that has gained recent attention is the β-barrel assembly machinery (BAM), which is conserved and essential across all Gram-negative bacteria. BAM is responsible for the biogenesis of β-barrel outer membrane proteins (β-OMPs) into the outer membrane. These β-OMPs serve essential roles for the cell including nutrient uptake, signaling, and adhesion, but can also serve as virulence factors mediating pathogenesis. The mechanism for how BAM mediates β-OMP biogenesis is known to be dynamic and complex, offering multiple modes for inhibition by small molecules and targeting by larger biologics. In this review, we introduce BAM and establish why it is a promising and exciting new therapeutic target and present recent studies reporting novel compounds and vaccines targeting BAM across various bacteria. These reports have fueled ongoing and future research on BAM and have boosted interest in BAM for its therapeutic promise in combatting multidrug resistance in Gram-negative bacterial pathogens.

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Evidence supporting an antimicrobial origin of targeting peptides to endosymbiotic organelles

Garrido

Caspari

Choquet

et al. 2020

Preprint

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Mitochondria and chloroplasts emerged from primary endosymbiosis. Most endosymbiont proteins have subsequently been expressed in the nucleo-cytosol of the host and organelle-targeted via the acquisition of N-terminal presequences, whose evolutionary origin remains enigmatic. Using a quantitative assessment of their physico-chemical properties, we show that organelle targeting peptides, which are distinct from signaling peptides targeting other subcellular compartments, group with a subset of antimicrobial peptides. We demonstrate that extant antimicrobial peptides target a fluorescent reporter to either the mitochondria or the chloroplast in the green alga Chlamydomonas reinhardtii and, conversely, that extant targeting peptides display antimicrobial activity. Thus, we provide conclusive computational and functional evidence for an evolutionary link between organelle-targeting and antimicrobial peptides. Our results support the view that resistance of bacterial progenitors of organelles to the attack of host antimicrobial peptides, has been instrumental in eukaryogenesis and in the subsequent emergence of photosynthetic eukaryotes.

show abstract

Identifying a potential receptor for the antibacterial peptide of sponge Axinella donnani endosymbiont

Cited by 3 publications

References 50 publications

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles

Targeting BAM for Novel Therapeutics against Pathogenic Gram-Negative Bacteria

Evidence supporting an antimicrobial origin of targeting peptides to endosymbiotic organelles

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