Identify biosorption effects of Thiobacillus towards perfluorooctanoic acid (PFOA): Pilot study from field to laboratory

Impact of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) on secondary sludge microorganisms: removal, potential toxicity, and their implications on existing wastewater treatment regulations in Canada

“…One study identified two obligate chemolithoautotrophic bacteria from river sediment that were exposed to high levels of PFOA due to being downstream of a fluorochemical facility. 22 This site's microbial diversity was much lower than the industrial areas, but the abundance of the Thiobacillus genus was high. 22 The two strands of this genus were Gram-negative obligate autotrophs: Thiobacillus thioparus – an aerobic bacterium, capable of oxidizing organic and inorganic sulphur compounds, and Thiobacillus denitrificans – a facultative anaerobe bacterium that can oxidize sulfides and reduce iron compounds.…”

“…22 This site's microbial diversity was much lower than the industrial areas, but the abundance of the Thiobacillus genus was high. 22 The two strands of this genus were Gram-negative obligate autotrophs: Thiobacillus thioparus – an aerobic bacterium, capable of oxidizing organic and inorganic sulphur compounds, and Thiobacillus denitrificans – a facultative anaerobe bacterium that can oxidize sulfides and reduce iron compounds. It was found that T. thioparus and T. denitrificans were able to remove up to 26.8% and 18.4% of PFOA, respectively.…”

“…22 This removal was associated entirely with adsorption as there were no observable changes in the pollutant's molecular conguration. 22 T. thioparus is larger than T. denitricans and its size could explain the higher biosorption of PFOA, because the cell walls of bacteria are commonly the main side for pollutant uptake. 22 To determine the effect of different cell wall structures on the PFAS removal, a study tested one Gram-positive facultative anaerobe bacterial species, Bacillus subtilis, and one Gramnegative heterotrophic bacteria, Pseudomonas putida.…”

“…22 T. thioparus is larger than T. denitricans and its size could explain the higher biosorption of PFOA, because the cell walls of bacteria are commonly the main side for pollutant uptake. 22 To determine the effect of different cell wall structures on the PFAS removal, a study tested one Gram-positive facultative anaerobe bacterial species, Bacillus subtilis, and one Gramnegative heterotrophic bacteria, Pseudomonas putida. 23 The difference between the two bacteria is that Gram-positive bacteria have no outer lipid membrane, only a peptidoglycan layer, while Gram-negative bacteria have both layers (peptidoglycan layer, sandwiched between inner and outer lipid bilayer membranes).…”

“…It was found that T. thioparus and T. denitrificans were able to remove up to 26.8% and 18.4% of PFOA, respectively. 22 This removal was associated entirely with adsorption as there were no observable changes in the pollutant's molecular configuration. 22 T. thioparus is larger than T. denitrificans and its size could explain the higher biosorption of PFOA, because the cell walls of bacteria are commonly the main side for pollutant uptake.…”