Identify and characterize the SNP markers for traceability of scallop spiny lobster Panulirus homarus (Decapoda, Palinuridae) in Vietnam

Abstract

Lobster is an aquatic species of ecological and economic importance. In Vietnam, ornate spiny lobster (Panulirus ornatus) and scallop spiny lobster (P. hormatus) are among the main species of aquaculture in Vietnam. Demand for lobster products domestically and internationally is increasing, as in the US, Japan and China markets. Today, consumers are more aware of product brands, food safety and hygiene, so product traceability is essential to protect consumers and businesses. The DNA markers are widely used for commercially traded seafood traceability. Among that, Single Nucleotide Polymorphisms (SNPs) have been shown to be robust markers, and offer high reliability. EzRAD sequencing is applied to detect potential SNPs that are characteristic for P. homarus populations in Vietnam and Sri Lanka. Through 211 valid SNPs, we detected and 12 SNPS occurring in Vietnam population, and designed 8 primer pairs to amplified 80-130 bp SNPs products. PCR optimization allows us to select 2 SNPs serving the traceability of Vietnamese P. homarus. Further need to more population sampling, and whole genome sequencing in order to develop SNPs panel to seeking the origin of lobster seafood products.

Traceability system for lobsters supply chain: a review finding and method

“…Traceability systems have developed rapidly, especially for food products because they are concerned with quality and safety. Based on Table 1, it is clear that the application of traceability systems to food products has been widely developed, although initially this system was widely applied to fishery products in the early nineties, due to high antibiotic contamination in shrimp and tuna products such as chloramphenicol along with the demands of global trade, this system has expanded to other fishery commodities, traceability has been successfully applied in the seafood industry such as in lobster, preliminary results show the application of lobster traceability [39]. The demands of global trade in terms of the quality of food products include the functional properties of each product, the level of freshness, and physical and biological contamination.…”