IDENTIFICAZIONE MOLECOLARE DI MUTAZIONI CONFERENTI RIFAMPICINA E ISONIAZIDE RESISTENZA IN M. tuberculosis IN CAMPIONI CLINICI DIRETTI

A large-volume sputum dry storage and transportation device for molecular and culture-based diagnosis of tuberculosis

“…Whatman FTA DBS cards and GenoCard (Hain Life Sciences), which are commercial nucleic acid preservation cards, have been tested for dry sputum storage in a few studies. [18][19][20] A filter paper-based device to concentrate Mtb bacteria for sputum microscopy and molecular drug susceptibility testing was described by Tyagi et al 21 TBSendan end to end solution for the collection and drying of sputum on filter paper and downstream molecular testing is being developed by Wobble Base Bioresearch. 22 An important limitation of all these approaches is the limited volume of sputum that can be dry stored and the fact that samples may only be recovered as punched membranes, as opposed to when in liquid form.…”