Identification with mitochondrial DNA typing from one sperm cell isolated by micromanipulation

Yükseloğlu, Emel Hülya; Bayhan, Esra; Rayimoğlu, Gülten; Çavuş, Fatma; Dastan, Kadir; Erkan, Itir

doi:10.5455/medscience.2018.07.8821

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the present study showed a correlation between the infertile sperms and specific ATP6 substitutions or mtDNA haplogroups, it could be possible that the obtained results exhibit forensic perspectives. Yukseloglu et al (2018) used the mtDNA typing of the sperm cells collected individually from a sexual assault victim and the mtDNA of the perpetrator sperms taken from the victim epithelium by micromanipulation technique for human identification. These authors used the sequence of the d-loop region, which is well-documented as a forensic maker.…”

Section: Discussionmentioning

confidence: 99%

Forensic Relationship between ATP6 Gene and Sperm Motility in a Saudi Population

Elsanousi¹,

Javed²,

Sufyan³

et al. 2020

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Approximately 600 bp of ATP synthase subunit 6 mitochondrial gene (ATP6) were amplified and sequenced for 110 semen samples collected from infertile and normal Saudi men inhabiting Riyadh province. These data were analyzed in order to identify the Single Nucleotide Polymorphisms (SNPs) associated with male infertility. Six silent substitutions G8697A, G8856A, T8889C, T8952C, C9075T and C9060A were recorded with one novel site (G8856A) in the infertile men. Seven non-synonymous substitutions were also obtained with 4 novel sites (C8684T, G8860A, C8876T and G9055A) in the infertile men. Nine haplogroups (H2a, H1a, H5a, T, J2a, K1a, N1b, M25 and U7) were predicted. H5a, M25 and U7 were found in the oligoasthenoteratospermic (AOT) infertile men only with a percentage of 4.5%. It could be concluded that there was a correlation between ATP6 substitutions/haplogroups and male infertility since specific SNPs and haplogroups appeared in the AOT infertile men only. The present results are considered preliminary for using the mitogenome of the infertile men in a forensic perspective. More mtDNA data from infertile men are necessary to assess mtDNA forensic contributions in male fertility. Examination of autosomal Short Tandem Repeats (STRs) and Y chromosome-STR profiles for infertile men should be conducted to ascertain any changes in the genetic loci.

show abstract

Section: Discussionmentioning

confidence: 99%

Forensic Relationship between ATP6 Gene and Sperm Motility in a Saudi Population

Elsanousi¹,

Javed²,

Sufyan³

et al. 2020

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

show abstract

“…The forensic applications for mtDNA include analysis of samples that are degraded or with low amount of DNA (e.g., stains, hairs, bones), and it was used for the identification of Tsar Nicholas II and his brother Georgij Romanov [67]. In recent approach, it has been demonstrated that the mtDNA could be used for the identification of sperm cells in the vaginal tract through a micromanipulation technique [68,69]. Besides the physical separation, sequence-specific primers (SSP) for the man were used to ensure that the woman's mtDNA would not be co-amplified.…”

Section: Mitochondrial Dna Analysismentioning

confidence: 99%

Biological Evidence Analysis in Cases of Sexual Assault

González¹,

Mercado²,

Salas³

et al. 2020

Biochemical Analysis Tools - Methods for Bio-Molecules Studies

View full text Add to dashboard Cite

Sexual assault (SA) is a crime of violence against a person's body resulting in a physical trauma, mental anguish, and suffering for victims generating expenses for government intended criminal investigation, medical care, and psychological attention. During the crime scene investigation, the identification and recovery of biological evidence (BE) are utmost important, since sometimes these are the only way to prove sexual contact and the perpetrator's identity. The examiner, with the help of specific technologies and techniques, must be able to find evidence that otherwise could go unnoticed. Forensic laboratories identify biological evidence with systemized protocols and use molecular methods to generate DNA profiles based on the amplification and DNA sequencing. Before the arrival of the new-generation sequencers, the application of other markers (single nucleotide polymorphisms (SNPs), insertion-deletion of nucleotides (INDEL), or microhaplotypes (MHs)) was laborious, expensive, and not very informative for forensic purposes; however, now they are useful in this field. Next-generation sequencing (NGS) brought a new series of applications like epigenetics, microbiota, messenger RNA, and microRNA analysis and the inferences in the ancestry and phenotyping of individuals. In the end, the results obtained from such analyses and stored in databases are very useful for the identification of sexual aggressors.

show abstract

Identification with mitochondrial DNA typing from one sperm cell isolated by micromanipulation

Cited by 2 publications

References 0 publications

Forensic Relationship between ATP6 Gene and Sperm Motility in a Saudi Population

Forensic Relationship between ATP6 Gene and Sperm Motility in a Saudi Population

Biological Evidence Analysis in Cases of Sexual Assault

Contact Info

Product

Resources

About