Identification to cardiac conduction cells in humans and pigs according to their zonal distribution, using histological, immunohistochemical and morphometric study.

Gómez-Torres, Fabián; Estupiñán, H. Yesid; Ruiz‐Saurí, Amparo

doi:10.1016/j.rvsc.2021.06.008

Cited by 1 publication

(1 citation statement)

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“…The pig has also become an attractive animal model for humans due to its genetic characteristics. Although the bone is less similar to humans due to its plexiformity [ 15 ], the gastrointestinal tract, the cardiovascular system [ 16 ] and the metabolism are more similar to humans than those of ruminants [ 17 ]. Furthermore, similarities between porcine and human MSCs have been described in the literature [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Human, Ovine and Porcine Mesenchymal Stem Cells from Bone Marrow: Critical In Vitro Comparison with Regard to Humans

Westerkowsky

Almeida

Selle

et al. 2023

Life

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For research and clinical use of stem cells, a suitable animal model is necessary. Hence, the aim of this study was to compare human-bone-marrow-derived mesenchymal stem cells (hBMSCs) with those from sheep (oBMSCs) and pigs (pBMSCs). The cells from these three species were examined for their self-renewal potential; proliferation potential; adhesion and migration capacity; adipogenic, osteogenic and chondrogenic differentiation potential; and cell morphology. There was no significant difference between hBMSCs and pBMSCs in terms of self-renewal potential or growth potential. The oBMSCs exhibited a significantly higher doubling time than hBMSCs from passage 7. The migration assay showed significant differences between hBMSCs and pBMSCs and oBMSCs—up to 30 min, hBMSCs were faster than both types and after 60 min faster than pBMSCs. In the adhesion assay, hBMSCs were significantly better than oBMSCs and pBMSCs. When differentiating in the direction of osteogenesis, oBMSCs and pBMSCs have shown a clearer osteogenic potential. In all three species, adipogenesis could only be evaluated qualitatively. The chondrogenic differentiation was successful in hBMSCs and pBMSCs in contrast to oBMSCs. It is also important to note that the cell size of pBMSCs was significantly smaller compared to hBMSCs. Finally, it can be concluded that further comparative studies are needed to draw a clear comparison between hBMSCs and pBMSCs/oBMSCs.

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