Identification, tissue distribution, periprandial expression, and anorexigenic effect of spexin in Siberian sturgeon, Acipenser baeri

Tian, Zhengzhi; Xu, Shaoqi; Wang, Mei; Wang, Yongdong; Chen, Hu; Tang, Ni; Wang, Bin; Zhang, Xin; Li, Zhiqiong

doi:10.1007/s10695-020-00856-y

Cited by 23 publications

(20 citation statements)

References 32 publications

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“…In this study, it was found that amylin in the hypothalamus and duodenum increased post-feeding significantly, which was consistent with the results in rats (Alam et al 1992). The identified anorexia factors, such as Urocortin-3 (Zhang et al 2016), CCK (Zhang et al 2017) and Spexin (Tian et al 2020), also increased significantly post-feeding.These results suggest that amylin may suppress appetite in Siberian sturgeon.…”

Section: Amylin Regulates Feedingsupporting

confidence: 90%

“…Further study on the feeding regulation of Siberian sturgeon may accelerate its growth, shorten the reproduction time and improve the quality of caviar. There have been studies on the regulation of feeding by CCK (Zhang et al 2017), UCN3 (Tang et al 2019) ,NPY (Yuan et al 2019) and Spexin (Tian et al 2020), but no report was found on the regulation by amylin in Siberian sturgeon. In order to study regulation of amylin on the feeding function of Siberian sturgeon, this study cloned amylin from Siberian sturgeon and analyzed its expression pattern.…”

Section: Introductionmentioning

confidence: 99%

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Amylin Inhibits the Feeding of the Siberian Sturgeon

Wang¹,

Xu²,

Li³

et al. 2021

Preprint

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Amylin is a 37-amino acid polypeptide that has been shown to be involved in feeding regulation in a few mammals, birds and goldfish. To study whether amylin regulates the feeding of Siberian sturgeon, this study cloned amylin of Siberian sturgeon and detected the distribution pattern of amylin in 15 tissues, the expression level in periprandial (pre- and post-feeding), and the changes of food intake and related appetite factors after intraperitonal injection experiment. The results showed that the expression of amylin was highest in hypothalamus, followed by duodenum, telencephalon, forebrain, midbrain, heart and liver, and low in cerebellum, pancreas, valvular intestine and other detection tissues. Compared with 1h pre-feeding, the expression level of amylin in hypothalamus was significantly increased at 1h post-feeding (P < 0.05), and the expression level of amylin in duodenum was extremely significantly increased at 1h post-feeding (P < 0.01). Compared with the control group (normal saline), the food intake at 0-1h and 1-3h after intraperitoneal injection of 100 ng/g BW and 200 ng/g BW was significantly decreased (P < 0.05), and the food intake at 3-6h in 100 ng/g BW group was extremely significantly decreased (P < 0.01), and the food intake at 3-6h in 200 ng/g BW group was significantly decreased (P < 0.05). After 1h of injection of 100 ng/g amylin, MC4R in hypothalamus was significantly increased (P < 0.05) and somatostatin was extremely significantly increased (P < 0.01), while amylin and NPY were significantly decreased (P < 0.05). CCK in valvular intestine was extremely significantly increased (P < 0.01), insulin in duodenum was significantly increased (P < 0.05), but ghrelin in duodenum had no significant change (P > 0.05). These results showed that Amylin inhibited feeding in Siberian sturgeon by down-regulating appetite stimulating factor NPY and up-regulating appetite suppressing factors somatostatin , MC4R , CCK and insulin .

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Section: Amylin Regulates Feedingsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Amylin Inhibits the Feeding of the Siberian Sturgeon

Wang¹,

Xu²,

Li³

et al. 2021

Preprint

Self Cite

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“…Besides, the SPX responses induced by feeding were mediated by the insulin signal triggered by glucose uptake (36) and our findings suggest that SPX can act as a novel satiety factor in fish species (5). This idea is also supported by similar studies in zebrafish (11), ya-fish (35), and more recently, in sturgeon (48). To test if the function of SPX as a satiety factor can also be observed in mammals, our study has been extended to the mouse model.…”

Section: Discussionsupporting

confidence: 58%

Mouse Spexin: (III) Differential Regulation by Glucose and Insulin in Glandular Stomach and Functional Implication in Feeding Control

Chen

He²,

Lei³

et al. 2021

Front. Endocrinol.

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Spexin (SPX), a neuropeptide with diverse functions, is a novel satiety factor in fish models and its role in feeding control has been recently confirmed in mammals. In mouse, food intake was shown to trigger SPX expression in glandular stomach with parallel rise in serum SPX and these SPX signals could inhibit feeding via central actions within the hypothalamus. However, the mechanisms for SPX regulation by food intake are still unclear. To examine the role of insulin signal caused by glucose uptake in SPX regulation, the mice were IP injected with glucose and insulin, respectively. In this case, serum SPX was elevated by glucose but not altered by insulin. Meanwhile, SPX transcript expression in the glandular stomach was up-regulated by glucose but the opposite was true for insulin treatment. Using in situ hybridization, the differential effects on SPX gene expression were located in the gastric mucosa of glandular stomach. Co-injection experiments also revealed that glucose stimulation on serum SPX and SPX mRNA expressed in glandular stomach could be blocked by insulin. In gastric mucosal cells prepared from glandular stomach, the opposite effects on SPX transcript expression by glucose and insulin could still be noted with similar blockade of the stimulatory effects of glucose by insulin. In this cell model, SPX gene expression induced by glucose was mediated by glucose uptake via GLUT, ATP synthesis by glycolysis/respiratory chain, and subsequent modulation of KATP channel activity, but the voltage-sensitive Ca2+ channels were not involved. The corresponding inhibition by insulin, however, was mediated by PI3K/Akt, MEK1/2/ERK1/2, and P38MAPK cascades coupled to insulin receptor but not IGF-1 receptor. Apparently, glucose uptake in mice can induce SPX expression in the glandular stomach through ATP synthesis via glucose metabolism and subsequent modification of KATP channel activity, which may contribute to SPX release into circulation to act as the satiety signal after food intake. The insulin rise caused by glucose uptake, presumably originated from the pancreas, may serve as a negative feedback to inhibit the SPX response by activating MAPK and PI3K/Akt pathways in the stomach.

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“…In the goldfish, spx1 mRNA expression increases in the telencephalon, optic tectum, and the hypothalamus, as well as in the periphery after feeding ( 39 , 46 ). The same effect is seen in the hypothalamus of Siberian sturgeon ( Acipenser baerii ), whereas in Ya-fish ( Schizothorax prenanti ), the increase is observed only in the forebrain ( 41 , 43 ). On the other hand, spx expression is responsive to food deprivation, however, effects vary according to species, experimental design, and probably metabolic conditions.…”

Section: Physiological Functions Of Spx and Galmentioning

confidence: 58%

“… Localization was obtained for orange-spotted grouper ( 34 ), zebrafish ( 35 , 40 ), goldfish ( 35 ), Nile tilapia ( 8 , 23 ), Ya fish ( 41 ), half smooth tongue sole ( 37 , 38 ), spotted scat ( 42 ), Siberian sturgeon ( 43 ). …”

Section: Introductionmentioning

confidence: 99%

Spexin and Galanin in Metabolic Functions and Social Behaviors With a Focus on Non-Mammalian Vertebrates

et al. 2022

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Spexin (SPX) and galanin (GAL) are two neuropeptides that are phylogenetically related and have descended from a common ancestral gene. Considerable attention has been given to these two multifunctional neuropeptides because they share GAL receptors 1,2, and 3. Since GAL and SPX-synthesizing neurons have been detected in several brain areas, therefore, it can be speculated that SPX and GAL are involved in various neurophysiological functions. Several studies have shown the functions of these two neuropeptides in energy regulation, reproduction, and response to stress. SPX acts as a satiety factor to suppress food intake, while GAL has the opposite effect as an orexigenic factor. There is evidence that SPX acts as an inhibitor of reproductive functions by suppressing gonadotropin release, while GAL modulates the activity of gonadotropin-releasing hormone (GnRH) neurons in the brain and gonadotropic cells in the pituitary. SPX and GAL are responsive to stress. Furthermore, SPX can act as an anxiolytic factor, while GAL exerts anti-depressant and pro-depressive effects depending on the receptor it binds. This review describes evidence supporting the central roles of SPX and GAL neuropeptides in energy balance, reproduction, stress, and social behaviors, with a particular focus on non-mammalian vertebrate systems.

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Identification, tissue distribution, periprandial expression, and anorexigenic effect of spexin in Siberian sturgeon, Acipenser baeri

Cited by 23 publications

References 32 publications

Amylin Inhibits the Feeding of the Siberian Sturgeon

Amylin Inhibits the Feeding of the Siberian Sturgeon

Mouse Spexin: (III) Differential Regulation by Glucose and Insulin in Glandular Stomach and Functional Implication in Feeding Control

Spexin and Galanin in Metabolic Functions and Social Behaviors With a Focus on Non-Mammalian Vertebrates

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