Identification SYT13 as a novel biomarker in lung adenocarcinoma

Abstract

Synaptotagmins are a class of proteins that play an important role in the secretion of neurotransmitters by synaptic vesicles. However, recent studies have shown that members of this family also have a certain function in the development of tumors. In this study, we first identified through The Cancer Genome Atlas data analyzed that a novel synaptotagmin, SYT13, was closely related to the prognosis of lung adenocarcinoma, but was not significantly correlated with the prognosis of lung squamous cell carcinoma. Then we knocked down the expression of SYT13 gene in lung adenocarcinoma cell lines A549 and H1299, and successfully induced decreased proliferation and clonality of lung adenocarcinoma cell lines, and observed cell cycle arrest and apoptosis enhancement in both cell lines. In addition, we detected the migration ability of SYT13 knockdown lung adenocarcinoma cell lines by the cell scratch test and the transwell test. Interestingly, there was a decreased migration ability of SYT13 knockdown in H1299 cells even though there was no significant difference in the migration of A549 cells. These results demonstrate that SYT13 plays an important role in the development of lung adenocarcinoma, which deepens our understanding of the mechanism of lung adenocarcinoma development and provides new possibilities for targeted therapy of lung adenocarcinoma.

A concurrent canonical and modified miRNAome pan-cancer study on TCGA and TARGET cohorts leads to an enhanced resolution in cancer

“…The copyright holder for this preprint this version posted May 20, 2021. ; https://doi.org/10.1101/2021.05.18.444694 doi: bioRxiv preprint SYT13 in breast cancer (TCGA-BRCA), an oncogenic gene in different cancers (116,117,(122)(123)(124)(125)(126)(127).…”

“…While the role of the canonical miR-381-3p is broadly acknowledged as a tumor suppressor (107,108), particularly in breast cancer (112,115), very little is known about the edited form in cancer (44,46). We investigate SYT13 in breast cancer (TCGA-BRCA), an oncogenic gene in different cancers (116,117,(122)(123)(124)(125)(126)(127).…”

“…After retaining significantly dysregulated genes (Supplementary Table 9) characterized by an opposite expression trend (miRNA down, genes up), we crossed them with the list of gene targets predicted by isoTar (Supplementary Table 9). Out of the reduced set of potential direct targets for miR-381-3p_4_A_G, we elected to study SYT13, an oncogene involved in different cancers (116,117). By using luciferase reporter vectors containing the 3' UTR of the gene and the two miRNA molecules (canonical and edited one) in HEK-293 cells, we demonstrated a direct binding between SYT13 and miR-381-3p_4_A_G (Figures 4G), as highlighted by the reduced luciferase activity of ~80% (Figure 4H).…”

Comprehensive analysis on expression profile and prognostic values of SYTs family members and their methylation in gastric cancer

“…However, several studies have recently reported that SYTs family members had vital roles in the pathogenesis of human cancer. It was discovered that SYT13 was up-regulated in the lung adenocarcinoma as well as in colorectal cancer, which promoted the lung adenocarcinoma cells and colorectal cancer cells proliferation and migration, and contributed to unsatisfactory prognosis 10,11 . Sung HY et al demonstrated that SYT2 was upregulated in ovarian cancer, promoted the ovarian carcinoma cells migration and invasiveness, and associated with poor survival for patients with ovarian cancer 12 .…”

“…Previous studies have showed that SYTs family members play an essential role in postsynaptic receptor endocytosis, vesicle tra cking, membrane repair, synaptic plasticity, and protection against neurodegeneration in the brain 7,8 . Additionally, several studies have recently discovered that SYTs family members play an oncogenic role in the pathogenesis and progression of human cancer 9,10 . However, the clinical and prognostic signi cance of SYTs family members in the occurrence and development of GC remain largely unknown.…”