Identification, Sequence, and Mapping of the Mouse Multiple PDZ Domain Protein Gene, Mpdz

Simpson, Eleanor H.; Suffolk, Ruth; Jackson, Ian J.

doi:10.1006/geno.1999.5853

Cited by 17 publications

(15 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, MUPP‐1 transcripts may be expressed as multiple‐spliced forms. We therefore next studied the tissue specific expression of the MUPP‐1 gene using probes derived from 5′ (nucleotide number 74–2083) or 3′ (4700–4980) (numbers are according to the cDNA sequence published in Simpson et al [17]). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

The direct association of the multiple PDZ domain containing proteins (MUPP‐1) with the human c‐Kit C‐terminus is regulated by tyrosine kinase activity

et al. 2000

View full text Add to dashboard Cite

We have identified the multiple PDZ domain containing protein (MUPP-1 or MPDZ) as a novel binding partner of the human c-Kit. c-Kit binds specifically to the 10th PDZ domain of MUPP-1 via its C-terminal sequence. Furthermore, a kinase negative-mutant receptor interacted more strongly with MUPP-1 than the wild-type c-Kit. Strikingly, a constitutively activated c-Kit (D816V-Kit) did not bind to MUPP-1, although this oncogenic form retains the PDZ binding motif`HDDV' at the C-terminal end. Deletion of V967 of c-Kit abolished binding to MUPP-1 and drastically reduced its tyrosine kinase activity, suggesting that the structure of the C-terminal tail of c-Kit influences its enzymatic activity. ß

show abstract

Section: Resultsmentioning

confidence: 99%

The direct association of the multiple PDZ domain containing proteins (MUPP‐1) with the human c‐Kit C‐terminus is regulated by tyrosine kinase activity

et al. 2000

View full text Add to dashboard Cite

show abstract

“…(19) Crb, Sdt and Dlt homologs are found in the C. elegans and mammalian genome. (1)(2)(3)5,19,(21)(22)(23) The Dlt homolog in C.…”

Section: Role Of the Crumbs Complex In Za Assemblymentioning

confidence: 99%

Adherens junctions: new insight into assembly, modulation and function

Tepaß

2002

BioEssays

View full text Add to dashboard Cite

Adherens junctions play pivotal roles in cell and tissue organization and patterning by mediating cell adhesion and cell signaling. These junctions consist of large multiprotein complexes that join the actin cytoskeleton to the plasma membrane to form adhesive contacts between cells or between cells and extracellular matrix. The best-known adherens junction is the zonula adherens (ZA) that forms a belt surrounding the apical pole of epithelial cells. Recent studies in Drosophila have further illuminated the structure of adherens junctions. Scaffolding proteins encoded by the stardust gene are novel components of the Crumbs complex, which plays a critical role in ZA assembly.1-3 The small GTPase Rap1 controls the symmetric re-assembly of the ZA after cell division.4 Finally, the asymmetric distribution of adherens junction material regulates spindle orientation during asymmetric cell division in the sensory organ lineage.

show abstract

“…For drug withdrawal convulsions, based upon positional cloning using robust behavioral models as well as sequence and expression analyses, the most compelling gene in the critical 1.8 Mb chromosomal segment is Mpdz . This gene and its human homolog encode the multi-PDZ domain protein (MPDZ, also called MUPP1) and show widespread expression in the brain (Ullmer et al, 1998;Simpson et al, 1999;Sitek et al 2003). The MPDZ protein is thought to affect neuronal excitability by altering the rate or fidelity of signal transduction mediated by a protein(s) with which it interacts, similar to other members of the PDZ domain protein family (Tsunoda et al, 1997;Fanning & Anderson, 1999;Sheng and Sala, 2001).…”

Section: Introductionmentioning

confidence: 99%

5-HT2C and GABAB receptors influence handling-induced convulsion severity in chromosome 4 congenic and DBA/2J background strain mice

et al. 2008

View full text Add to dashboard Cite

Progress towards elucidating the underlying genetic variation for susceptibility to complex central nervous system (CNS) hyperexcitability states has just begun. Genetic mapping analyses suggest that a gene(s) on mid-chromosome 4 has pleiotropic effects on multiple CNS hyperexcitability states in mice, including alcohol and barbiturate withdrawal and convulsions elicited by chemical and audiogenic stimuli. We recently identified Mpdz within this chromosomal region as a gene that influences alcohol and barbiturate withdrawal convulsions. Mpdz encodes the multi-PDZ domain protein (MPDZ). Currently, there is limited information available about the mechanism by which MPDZ influences drug withdrawal and/or other CNS hyperexcitability states, but may involve its interaction with 5-HT 2C and/or GABA B receptors. One of the most useful tools we have developed thus far is a congenic strain that possesses a segment of chromosome 4 from the C57BL/6J (donor) mouse strain superimposed on a genetic background that is >99% from the DBA/2J strain. The introduced segment spans the Mpdz gene. Here, we demonstrate that handling-induced convulsions are less severe in congenic vs. background strain mice in response to either a 5-HT 2C receptor antagonist (SB242084) or a GABA B receptor agonist (baclofen), but not a GABA A receptor channel blocker (pentylenetetrazol). These data suggest that allelic variation in Mpdz, or a linked gene, influences SB242084-and baclofen-enhanced convulsions. Our results are consistent with the hypothesis that Mpdz's effects on CNS hyperexcitability, including alcohol and barbiturate withdrawal, involve MPDZ interaction with 5-HT 2C and/or GABA B receptors. However, additional genes reside within the congenic interval and may also influence CNS hyperexcitability.

show abstract

Identification, Sequence, and Mapping of the Mouse Multiple PDZ Domain Protein Gene, Mpdz

Cited by 17 publications

References 15 publications

The direct association of the multiple PDZ domain containing proteins (MUPP‐1) with the human c‐Kit C‐terminus is regulated by tyrosine kinase activity

The direct association of the multiple PDZ domain containing proteins (MUPP‐1) with the human c‐Kit C‐terminus is regulated by tyrosine kinase activity

Adherens junctions: new insight into assembly, modulation and function

5-HT2C and GABAB receptors influence handling-induced convulsion severity in chromosome 4 congenic and DBA/2J background strain mice

Contact Info

Product

Resources

About