Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex

Optimizing storage conditions to prevent cold denaturation of trypsin for sequencing and to prolong its shelf life

“…The enzymatic activity of trypsin stored under different conditions was tested using BAPNA as a substrate in a 96-well microplate (Sarstedt, Numbrecht, Germany) as described [17], with some modifications. Briefly, 50 L of 100 times diluted enzyme solution was added to 100 L of substrate solution (1 mM BAPNA in 100 mM Tris buffer pH 8.0).…”

Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage

“…Biological activity of purified papain was tested using BAPNA (Sigma-Aldrich, Steinheim, Germany) as a substrate in a 96-well microtitre plate (Sarstedt, Numbrecht, Germany) as described in Raskovic et al [22] with some modifications. Briefly, 100 µL substrate solution (1mM BAPNA in 100 mM Tris buffer pH 8.0 with 1 mM EDTA and 10 mM L-cysteine) was mixed with aqueous papain solution (25 µL) incubated at 25°C.…”

Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening

“…Proteolytic fractions of latices of the genus Ficus predominantly contain cysteine proteases, but there are also other classes of proteases, e.g. aspartic and serine proteases …”

“…It is known that there are multiple forms of this enzyme in fig latex, and all of them belong to the cysteine protease class . Ficin from fig latex is known as an enzyme with milk clotting activity (ancient writings such as the Iliad indicate that fig latex can be used for cheese‐making), but it also has high proteolytic activity over a broad temperature and pH range . Apart from ficin, fig latex also contains the newly described collagenolytic serine protease, as well as a few recently described chitinolytic enzymes …”