Identification, protein antiglycation, antioxidant, antiproliferative, and molecular docking of novel bioactive peptides produced from hydrolysis of Lens culinaris

Abstract

Bioactive peptides produced from natural sources are considered as strategic target for drug discovery. Hyperglycemia caused protein glycation alters the structure of many tissues that impairs their functions and lead complications diseases in human body. This study investigated the bioactive peptides produced from red and brown Lens culinaris that might inhibit protein glycation to prevent diabetic complications. In this study, red and brown Lens culinaris protein hydrolysates were prepared by tryptic digestion, using an enzyme/substrate ratio of 1:20 (g/g), at 37°C, 12 hr then peptide fractions <3 kDa were filtered by using ultrafiltration membranes. Protective ability against protein glycation, DPPH radical scavenging, and anti-proliferative activities (on HepG2, MCF-7, and PC3 cell lines) of peptide fractions were assayed in vitro. Results showed that glycation was inhibited by peptides from 28.1% to 68.3% in different test model. PC3 cell line was more sensitive to the peptides which showed strong anticancer activity with lower IC50 (0.96 mg/ml). Peptide fractions were sequenced by HPLC-MS-MS. Twenty eight novel peptides sequences was identified. In silico study, two peptides could be developed as a potential bioactive peptides exhibited antiglycation, antioxidant, and antiproliferative activities. Practical applications Peptides are becoming an emerging source of medications with the development of new technologies. We have selected Lens Culinaris as one of the rich sources of proteins to explore novel bioactive peptides encapsulated in its seeds. Peptides fractions demonstrated protective ability against protein glycation, strong antioxidant potential, and promising antiproliferative activity. We have identified 28 novel peptides and molecular docking study revealed that some peptides showed strong binding potential to insulin receptor and ACE. Thus, these peptides might be used to manage diabetes complication as well as COVID-19 disease due to their interaction with ACE. However, those peptides needs to be further studied as a potential new drug.

Maillard reaction between oligopeptides and reducing sugar at body temperature: The putative anti-glycation agents

“…The analysis of amino acids, dipeptides, and GSH was based on their derivatization with o-phthaldialdehyde (OPA) to form a high fluorescent adduct, following the method of Kuerban et al ( 11 ). In brief, derivatizations of blank and sample solutions were performed by mixing 0.2 mL sample solution with 1.5 mL OPA solution, which was then stirred thoroughly.…”

Application of emerging technologies to obtain legume protein isolates with improved techno‐functional properties and health effects

“…Enzymatic digestion of black beans, green peas, chickpeas, and lentils has shown 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase (HMGR) and pancreatic lipase (PL) inhibitory activity, with different and synergistic effects (Moreno et al., 2020). Inhibition of protein glycation, which could be related to the prevention of complications in diabetes, has been suggested for lentils (Kuerban et al., 2020). In vitro α‐amylase inhibition has been observed in pigeon peas (Olagunju et al., 2020).…”

“…Black bean, green pea, chickpea, lentil (Moreno et al., 2020), and pigeon pea hydrolysates (Olagunju et al., 2020) also possess antioxidant activity. In vitro studies in different cell lines suggested the antiproliferative effects of lentils, which suggested potential anticancer effects (Kuerban et al., 2020). Thus, there is a wide spectrum of enzymatic inhibitory activities of legume hydrolysates, which point to them as an interesting source of bioactive peptides.…”

A review on the techno‐functional, biological, and health‐promoting properties of hempseed‐derived proteins and peptides

“…Changes in intracellular biomolecules such as mitochondria, endoplasmic reticulum, membrane lipid, proteins, and DNA may result from abnormal ROS accumulation, resulting in decreased cell viability (Liu et al, 2020). Bioactive proteins derived from legumes, marine organisms and some agricultural wastes, and peptides isolated from them represent emerging anticancer entities due to their remarkable selective cytotoxicity against cancer cells while sparing normal cells (Cicero et al, 2017; Kuerban et al, 2020; Moon et al, 2017). Recently, the effects of HSPs hydrolyzed with papain‐neutral protease against liver cancer (Hep3B) and normal liver (L02) cells were investigated (Wei et al, 2021).…”