Identification, prevalence and pathogenicity of Colletotrichum species causing anthracnose of Capsicum annuum in Asia

Silva, Dilani D. de; Groenewald, Johannes Z.; Crous, Pedro W.; Ades, Peter K.; Nasruddin, Andi; Mongkolporn, Orarat; Taylor, P. W. J.

doi:10.1186/s43008-019-0001-y

Cited by 122 publications

(111 citation statements)

References 53 publications

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“…The gloeosporioides complex currently comprises 49 accepted species based on phylogenetic analyses and morphological approaches, with pathogenic and endophytic species known in a variety of hosts (Jayawardena et al ., 2016; Guarnaccia et al ., 2017; Sharma et al ., 2017; Vieira et al ., 2017; De Silva et al ., 2019). Although it has been argued that C .…”

Section: Discussionmentioning

confidence: 99%

“…The acutatum complex comprises 38 accepted species, all isolated from anthracnose‐affected crops worldwide (Jayawardena et al ., 2016; De Silva et al ., 2019; Damm et al ., 2020). C .…”

Section: Discussionmentioning

confidence: 99%

“…Knowledge of the diversity of Colletotrichum species associated with a host is crucial for the implementation of efficient management strategies because some species are better adapted than others in the field. Habituation of some Colletotrichum species is enhanced by factors such as high or inherent resistance to fungicides (Chen et al ., 2018; Fuentes‐Aragón et al ., 2020), the development of sexual morphs that are epidemiologically important for genetic recombination, or virulence grades (Sharma et al ., 2017; De Silva et al ., 2019; Fuentes‐Aragón et al ., 2020). In addition, monitoring quarantined pathogens requires precise identification to avoid spreading (Weir et al ., 2012; Crous et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

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Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

et al. 2020

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Colletotrichum species cause anthracnose disease in tropical and subtropical fruit crops worldwide. Mexico is the main producer and exporter of avocado (Persea americana) globally and has yearly outbreaks of anthracnose on this crop. However, which specific Colletotrichum spp. cause these outbreaks in avocado‐producing regions remain uncertain; thus, the objective of the present study was to identify the species responsible. A survey performed in six production regions of Mexico yielded 232 isolates, from which a subset of 104 strains was selected based on morphological characteristics and origin. This subset was sequenced and haplotypes were analysed in the gapdh partial gene. Finally, 31 strains were identified through multilocus phylogenetic analyses using the sequences of the internal transcribed spacer region and six loci. This study revealed the presence of two species previously reported in Mexico (C. karsti and C. godetiae), three novel records in Mexico (C. siamense, C. fioriniae, and C. cigarro), four novel records on avocado (C. chrysophilum, C. jiangxiense, C. tropicale, and C. nymphaeae), and two novel lineages (Colletotrichum sp. 1 and Colletotrichum sp. 2). C. siamense was the most prevalent, while the species reported for the first time on avocado, including the novel lineages, were the least prevalent. C. karsti was the most widespread (four regions), followed by C. siamense, C. jiangxiense, and C. chrysophilum (three regions). Pathogenicity tests showed that all species caused anthracnose on avocado fruit. These findings will be useful for improving the management of avocado anthracnose outbreaks in Mexico.

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Section: Discussionmentioning

confidence: 99%

“…The acutatum complex comprises 38 accepted species, all isolated from anthracnose‐affected crops worldwide (Jayawardena et al ., 2016; De Silva et al ., 2019; Damm et al ., 2020). C .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

et al. 2020

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“…As a physical barrier, the cuticle surrounds the epidermis of fruits and serves a number of functions including (a) to protect the plant against the physical, environmental and biological stresses and pathogen invasion, (b) to reduce the effects of internal water loss, and (c) to maintain plant organ integrity by providing mechanical support (Belge et al, ; Chen et al, ; Lara, Belge, & Goulao, ). In pathogenicity tests, C. truncatum isolates are pathogenic on Capsicum fruits after wounding the fruit surface, and most produced a low level of infection on nonwounded fruit (De Silva et al, ; Ramdial & Rampersad, ). The life cycle of C. truncatum in Capsicum sp.…”

Section: Introductionmentioning

confidence: 99%

“…C. capsici ‐ Damm, Woudenberg, Cannon, & Crous, ; Ramdial & Rampersad, ) and more recently by C. brevisporum , albeit with lower incidence compared to C. truncatum (Villafana, Ramdass, & Rampersad, ). C. truncatum was the predominant species of Colletotrichum associated with anthracnose of chili in Asia and is widely distributed throughout Asia, Australia, and South America (De Silva, Ades, Crous, & Taylor, ; De Silva et al, ; Diao et al, ; Mongkolporn & Taylor, ; Sharma, Kumar Pinnaka, & Shenoy, ). Multilocus phylogeny revealed that the recently characterized Colletotrichum magnum species complex consists of nine closely‐related species which includes C. brevisporum (Damm et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Diversity, structure, and synteny of the cutinase gene ofColletotrichumspecies

Villafana

Rampersad

2020

Ecology and Evolution

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Colletotrichum species complexes are among the top 10 economically important fungal plant pathogens worldwide because they can infect climacteric and nonclimacteric fruit at the pre and/or postharvest stages. C. truncatum is the major pathogen responsible for anthracnose of green and red bell pepper fruit worldwide. C. brevisporum was recently reported to be a minor pathogen of red bell pepper fruit in Trinidad, but has recently been reported as pathogenic to other host species in other countries. The ability of these phytopathogens to produce and secrete cutinase is required for dismantling the cuticle of the host plant and, therefore, crucial to the necrotrophic phase of their infection strategy. In vitro bioassays using different lipid substrates confirmed the ability of C. truncatum and C. brevisporum isolates from green and red bell peppers to secrete cutinase. The diversity, structure and organization and synteny of the cutinase gene were determined among different Colletotrichum species. Cluster analysis indicated a low level of nucleotide variation among C. truncatum sequences. Nucleotide sequences of C. brevisporum were more related to C. truncatum cutinase nucleotide sequences than to C. gloeosporioides. Cluster patterns coincided with haplotype and there was evidence of significant positive selection with no recombination signatures. The structure of the cutinase gene included two exons with one intervening intron and, therefore, one splice variant. Although amino acid sequences were highly conserved among C. truncatum isolates, diversity “hot spots” were revealed when the 66‐amino acid coding region of 200 fungal species was compared. Twenty cutinase orthologues were detected among different fungal species, whose common ancestor is Pezizomycotina and it is purported that these orthologues arose through a single gene duplication event prior to speciation. The cutinase domain was retained both in structure and arrangement among 34 different Colletotrichum species. The order of aligned genomic blocks between species and the arrangement of flanking protein domains were also conserved and shared for those domains immediately located at the N‐ and C‐terminus of the cutinase domain. Among these were an RNA recognition motif, translation elongation factor, signal peptide, pentatricopeptide repeat, and Hsp70 family of chaperone proteins, all of which support the expression of the cutinase gene. The findings of this study are important to understanding the evolution of the cutinase gene in C. truncatum as a key component of the biotrophic–necrotrophic switch which may be useful in developing gene‐targeting strategies to decrease the pathogenic potential of Colletotrichum species.

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First report of Colletotrichum scovillei causing anthracnose of pepper in Kosovo

Xhemali

Cortiello

Gjinovci³

et al. 2023

New Disease Reports

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Identification, prevalence and pathogenicity of Colletotrichum species causing anthracnose of Capsicum annuum in Asia

Cited by 122 publications

References 53 publications

Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

Colletotrichum species causing anthracnose on avocado fruit in Mexico: Current status

Diversity, structure, and synteny of the cutinase gene ofColletotrichumspecies

First report of Colletotrichum scovillei causing anthracnose of pepper in Kosovo

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