Identification, phylogeny and transcript profiling of ERF family genes during temperature stress treatment in Pea (Pisum sativum L.)

Sharma, Samriti; Chahal, Anuraj; Prasad, Heerendra; Walia, Abhishek; Kumar, Raj; Dobhal, Sneha

doi:10.1007/s13562-021-00709-6

Cited by 8 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ERF60 and ERF041, AP2/ERF family TF, were induced by high temperatures, with the highest induction multiplicity of 6-fold and 2-fold, respectively (Figure 4). ERF60 has an important role in response to temperature stress in pea using RNA-seq [31]. Moreover, ERF60 overexpression enhances drought and salt tolerance in Arabidopsis seedlings [32].…”

Section: Discussionmentioning

confidence: 99%

Rapid Identification of High-Temperature Responsive Genes Using Large-Scale Yeast Functional Screening System in Potato

Wang,

Wen,

Shang

et al. 2023

Plants

View full text Add to dashboard Cite

As the third largest global food crop, potato plays an important role in ensuring food security. However, it is particularly sensitive to high temperatures, which seriously inhibits its growth and development, thereby reducing yield and quality and severely limiting its planting area. Therefore, rapid, and high-throughput screening for high-temperature response genes is highly significant for analyzing potato high-temperature tolerance molecular mechanisms and cultivating new high-temperature-tolerant potato varieties. We screened genes that respond to high temperature by constructing a potato cDNA yeast library. After high-temperature treatment at 39 °C, the yeast library was subjected to high-throughput sequencing, and a total of 1931 heat resistance candidate genes were screened. Through GO and KEGG analysis, we found they were mainly enriched in “photosynthesis” and “response to stimuli” pathways. Subsequently, 12 randomly selected genes were validated under high temperature, drought, and salt stress using qRT-PCR. All genes were responsive to high temperature, and most were also induced by drought and salt stress. Among them, five genes ectopically expressed in yeast enhance yeast’s tolerance to high temperatures. We provide numerous candidate genes for potato response to high temperature stress, laying the foundation for subsequent analysis of the molecular mechanism of potato response to high temperature.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid Identification of High-Temperature Responsive Genes Using Large-Scale Yeast Functional Screening System in Potato

Wang,

Wen,

Shang

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Plant breeders have developed heattolerant pea cultivars that are better adapted to high-temperature conditions. These cultivars possess genetic traits that enable them to withstand heat stress and maintain better growth and yield compared to traditional varieties (Sharma et al, 2022). Also, certain growth regulators, such as cytokinins can stimulate plant growth and yield even under high-temperature conditions (Abd El-Hady et al, 2016;Mok, 2019), as this hormone promotes cell division, elongation, and flowering, which can enhance the overall productivity of pea plants.…”

Section: Introductionmentioning

confidence: 99%

Effect of some Treatments Stimulating Growth and Yield on Pea Plants Grown under High Temperature Conditions

Ahmed

Taha

2023

Journal of Plant Production

View full text Add to dashboard Cite

To investigate the impact of some stimulants on the pea plants grown under high-temperature conditions, a field experiment was conducted to assess the effects of vitamin C, melatonin, potassium citrate, and cytokinin, in addition to a control group that did not receive any spray, on pea cultivars including A (master b), B (sweet 1), and C (sweet 2), which were assigned to the main plots. While, the stimulants were arranged in the sub-main plots. The experiment measured various growth and yield parameters, including plant height, leaf area, fresh and dry weights, chlorophyll content, carotene content, days required for fruit setting, No. of pods, pod length, pod yield and protein content. The results showed that the cultivar "C" performed the best across all studied treatments, except for days required for fruit setting. Following "C" cultivar, the performance of cultivar "B" was observed to be superior, while cultivar "A" ranked third in terms of growth and yield characteristics. In terms of fruit setting, cultivars "A" and "B" exhibited early fruit setting, whereas cultivar "C" experienced a delay in fruit set. Regarding the stimulants, spraying cytokinin yielded the highest values for all the studied characteristics. Potassium citrate treatment ranked second in terms of performance, followed by melatonin then vitamin C treatments, respectively, while the control group showed the lowest values. Overall, the application of cytokinin had the most positive impact on the growth and yield of pea plants under high-temperature conditions. Other studied stimulants also had positive effects, although to a lesser extent.

show abstract

“…It has been known for a long time that the ERF gene family is involved in many processes, such as plant growth and development [10,19] and abiotic or biotic stress [20,21]. Samriti et al found that some of the Pisum sativum ERF gene family could resist cold stress [22]. Klay et al also found a similar function in Solanum lycopersicum [23].…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Analysis of the Ethylene Response Factor Gene Family in Tea Plant (Camellia sinensis)

et al. 2023

View full text Add to dashboard Cite

The ERF gene family is widely present in plants and has crucial regulatory importance in plant seed development, organ morphogenesis, the synthesis of secondary metabolites, and coping with abiotic stresses such as cold and drought. In this study, 90 members of CsERF were screened by bioinformatics tools analysis and named CsERF1–CsERF90. Their molecular characteristics and systematic evolution were studied, and the tissue expression characteristics of CSERF genes and the composition of promoter cis-acting elements were predicted. The results showed that 81 proteins encoded by CsERF genes had conserved motifs 1, 2, and 3, while 64 members possessed other motifs. The theoretical isoelectric point was between 4.49 and 10.24, and 85 members constituted unstable proteins, while the rest were stable proteins. Subcellular localization predicted that 77 members were in the nucleus, 8 were in the chloroplasts, and 5 were in the mitochondria. The promoter sequence of CsERFs was found to include not only cis-acting elements related to hormone regulation, such as gibberellin (41), methyl jasmonate (110), and abscisic acid (185), but also cis-acting elements involved in low-temperature response (56) and light response (22), indicating that CsERFs have a key role in plant growth and abiotic stress. Phylogenetic analysis of tea plant and Arabidopsis thaliana ERF gene families showed that the tea plant ERF gene families could be divided into six groups, with B3 having 29 members at most and B1 having only 3 members at least. The phylogenetic tree constructed using only the CsERF genes is also divided into six groups, with slightly different but minimal differences in members. Of the 90 tea plant ERF members, 85 were located on 15 chromosomes, whereas 5 were not located on chromosomes. The collinearity analysis showed that there were 41 homologous gene pairs among the CsERFs, and these homologous gene pairs may have the same function. According to the expression of CsERFs in cold-stressed tea plant and in different tissues, 90 CsERF genes played their respective roles in different tissues and stages to regulate plant growth, and some of them participated in the process of cold stress tolerance. This study provides a theoretical foundation for the study of tea plant growth and development and low-temperature resistance.

show abstract

Identification, phylogeny and transcript profiling of ERF family genes during temperature stress treatment in Pea (Pisum sativum L.)

Cited by 8 publications

References 45 publications

Rapid Identification of High-Temperature Responsive Genes Using Large-Scale Yeast Functional Screening System in Potato

Rapid Identification of High-Temperature Responsive Genes Using Large-Scale Yeast Functional Screening System in Potato

Effect of some Treatments Stimulating Growth and Yield on Pea Plants Grown under High Temperature Conditions

Identification and Expression Analysis of the Ethylene Response Factor Gene Family in Tea Plant (Camellia sinensis)

Contact Info

Product

Resources

About