Identification ofStaphylococcus piscifermentans from dog feces

Abstract

The AD 2 strain isolated from feces of a healthy dog in Slovakia was characterized phenotypically by the conventional tests and commercial identification kits API Staph and ID32 Staph. Results of biochemical tests identified the strain as S. piscifermentans, fully corresponding with the species description. Further characterization by whole-cell protein profile analysis (SDS-PAGE) confirmed the identification based on biochemical tests and showed that the AD 2 strain is S. piscifermentans; lactic acid production, urease activity, bacteriocin production and the antibiotic susceptibility of it were also determined. S. piscifermentans AD 2 isolated first from an animal source was deposited in the Czech Collection of Microorganisms as Staphylococcus piscifermentans CCM 7165.

Cytotoxic and Other Metabolites of Aspergillus Inhabiting the Rhizosphere of Sonoran Desert Plants

“…Compounds 4 − 6 have been reported to inhibit sea urchin embryogenesis by acting on components of the mitotic apparatus, and a recent patent application suggests their weak interaction with heat shock protein 90 (Hsp90), a promising target for anticancer drug discovery . Penicillic acid ( 9 ) has been reported to be cytotoxic to A2780 human ovarian carcinoma,22a Chinese hamster ovary cells,22b and HeLa cells 22c and was found to inhibit the growth of sarcoma originally induced by it 22d22e induction of DNA single-strand breaks,22b and inhibition of DNA synthesis 22c.…”

“…Penicillic acid ( 9 ) has been reported to be cytotoxic to A2780 human ovarian carcinoma,22a Chinese hamster ovary cells,22b and HeLa cells 22c and was found to inhibit the growth of sarcoma originally induced by it 22d22e induction of DNA single-strand breaks,22b and inhibition of DNA synthesis 22c. It has recently been demonstrated that penicillic acid ( 9 ) inhibits Fas ligand-induced apoptosis by targeting self-processing of caspase-8 22f.…”

“…The mechanism of cytotoxicity of 9 has been determined to be due to its ability to bind to SH groups in macromolecules,22e induction of DNA single-strand breaks,22b and inhibition of DNA synthesis 22c. It has recently been demonstrated that penicillic acid ( 9 ) inhibits Fas ligand-induced apoptosis by targeting self-processing of caspase-8 22f. The absence of any cytotoxic activity of its derivatives 8 and 16 suggests the requirement of the isopropenyl moiety for the cytotoxicity of 9 .…”