Identification of β-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods

Keserű, Judit; Szabó, István; Gál, Zsuzsanna; Massidda, Orietta; Mingoia, Marina; Kaszanyitzky, Éva; Jánosi, Szilárd; Hulvely, Julianna; Csorba, Attila; Buzás, Krisztina; Hunyadi‐Gulyás, Éva; Medzihradszky, Katalin F.; Bíró, Sándor

doi:10.1016/j.vetmic.2010.06.006

Cited by 6 publications

(5 citation statements)

References 19 publications

(2 reference statements)

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“…Proteins from the fermentation broth were separated by 2D gel electrophoresis [51]. Selected spots were cut from gels manually, and, after trypsin digestion, peptides were analyzed on a 4000 QTRAP (AB Sciex, Framingham, MA, USA) mass spectrometer coupled to an Easy nLC II nanoHPLC (Bruker, Billerica, MA, USA).…”

Section: Identification Of Extracellular Proteinsmentioning

confidence: 99%

Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans

Gila

Antal

Birkó

et al. 2022

JoF

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Understanding the coordinated regulation of the hundreds of carbohydrate-active enzyme (CAZyme) genes occurring in the genomes of fungi has great practical importance. We recorded genome-wide transcriptional changes of Aspergillus nidulans cultivated on glucose, lactose, or arabinogalactan, as well as under carbon-starved conditions. We determined both carbon-stress-specific changes (weak or no carbon source vs. glucose) and carbon-source-specific changes (one type of culture vs. all other cultures). Many CAZyme genes showed carbon-stress-specific and/or carbon-source-specific upregulation on arabinogalactan (138 and 62 genes, respectively). Besides galactosidase and arabinan-degrading enzyme genes, enrichment of cellulolytic, pectinolytic, mannan, and xylan-degrading enzyme genes was observed. Fewer upregulated genes, 81 and 107 carbon stress specific, and 6 and 16 carbon source specific, were found on lactose and in carbon-starved cultures, respectively. They were enriched only in galactosidase and xylosidase genes on lactose and rhamnogalacturonanase genes in both cultures. Some CAZyme genes (29 genes) showed carbon-source-specific upregulation on glucose, and they were enriched in β-1,4-glucanase genes. The behavioral ecological background of these characteristics was evaluated to comprehensively organize our knowledge on CAZyme production, which can lead to developing new strategies to produce enzymes for plant cell wall saccharification.

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Section: Identification Of Extracellular Proteinsmentioning

confidence: 99%

Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans

Gila

Antal

Birkó

et al. 2022

JoF

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“…In S. aureus , a unique enzyme, the penicillinase encoded by the blaZ gene, is responsible for penicillin degradation. The only study of penicillinase detection by proteomic methods was performed using bovine and human isolates of borderline oxacillin‐resistant S. aureus strains . Using a gel‐based approach, the authors identified BlaZ enzymes and a longer variant with high sequence coverages of 22 to 79% in the supernatant fraction and of 18 to 82% in the membrane‐bound fraction.…”

Section: Antibiotic Resistance Predictionmentioning

confidence: 99%

“…However, Keseru et al. showed that the mechanism responsible for S. aureus low‐level or borderline methicillin resistance was due to the production of penicillinases similar to BlaZ . They also observed slight sequence variations in these proteins that might account for their different sizes and substrate specificity.…”

Section: Antibiotic Resistance Predictionmentioning

confidence: 99%

Mass spectrometry methods for predicting antibiotic resistance

Charretier

Schrenzel

2016

Proteomics Clinical Apps

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Developing elaborate techniques for clinical applications can be a complicated process. Whole-cell MALDI-TOF MS revolutionized reliable microorganism identification in clinical microbiology laboratories and is now replacing phenotypic microbial identification. This technique is a generic, accurate, rapid, and cost-effective growth-based method. Antibiotic resistance keeps emerging in environmental and clinical microorganisms, leading to clinical therapeutic challenges, especially for Gram-negative bacteria. Antimicrobial susceptibility testing is used to reliably predict antimicrobial success in treating infection, but it is inherently limited by the need to isolate and grow cultures, delaying the application of appropriate therapies. Antibiotic resistance prediction by growth-independent methods is expected to reduce the turnaround time. Recently, the potential of next-generation sequencing and microarrays in predicting microbial resistance has been demonstrated, and this review evaluates the potential of MS in this field. First, technological advances are described, and the possibility of predicting antibiotic resistance by MS is then illustrated for three prototypical human pathogens: Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Clearly, MS methods can identify antimicrobial resistance mediated by horizontal gene transfers or by mutations that affect the quantity of a gene product, whereas antimicrobial resistance mediated by target mutations remains difficult to detect.

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“…Another type of oxacillin-resistant S. aureus is the borderline oxacillin-resistant S. aureus (BORSA) phenotype that contains alternative resistance mechanisms. These strains include β-lactamasehyperproducing S. aureus and S. aureus with point mutations in other PBP genes [13][14][15][16][17][18]. There are no strict laboratory diagnostic criteria to define these resistance phenotypes.…”

Section: Introductionmentioning

confidence: 99%

“…aureus and S. aureus with point mutations in other PBP genes [13–18]. There are no strict laboratory diagnostic criteria to define these resistance phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Hospital transmission of borderline oxacillin-resistant Staphylococcus aureus evaluated by whole-genome sequencing

Konstantinovski

Veldkamp

Lavrijsen

et al. 2021

Journal of Medical Microbiology

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Introduction. Staphylococcus aureus is a major cause of hospital infections worldwide. Awareness towards methicillin-resistant S. aureus (MRSA) infections is high but attention towards borderline oxacillin-resistant S. aureus (BORSA) is limited, possibly due to an underestimated clinical relevance, presumption of low incidence and diagnostic limitations. Gap statement. BORSA surveillance has not been routinely implemented, and thus consensus with regard to a definition and infection control measures is lacking. Aim. Our goals were to investigate the occurrence, molecular characteristics and clinical manifestations of BORSA infections in the hospital setting. Methodology. Following an increased incidence in 2016, BORSA cases in 2014/2016 (in our institution) were more specifically evaluated. Medical records were reviewed to investigate epidemiological links, clinical characteristics and outcomes. Resistance and virulence markers were assessed by whole genome sequencing (WGS). Conventional methods: amplified fragment length polymorphism (AFLP) ; multilocus sequence typing (MLST) and multiple locus variable-number tandem repeat analysis (MLVA) were compared with core genome MLST (cgMLST) and whole-genome single nucleotide polymorphism (wgSNP) analysis to confirm genetic clusters. Results. From 2009 to 2013, BORSA comprised 0.1 % of all clinical S. aureus strains. In 2016, the incidence was six-fold higher in comparison to the baseline. Whole-genome SNP and cgMLST confirmed two BORSA clusters among patients with dermatological conditions. Patients with BORSA presented with skin infections, and one case developed a severe invasive infection with a fatal outcome. Infection control measures successfully prevented further transmission in both clusters. WGS findings showed that BORSA strains carried multiple resistance and virulence genes with increased pathogenic potential. Conclusion. WGS and cgMLST effectively characterized and confirmed BORSA clusters among at-risk patients with clinical manifestations ranging from mild skin infections to life-threatening bacteraemia. Clinical awareness and active monitoring are therefore warranted for the timely implementation of infection control measures to prevent BORSA transmission in high-risk patients.

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Identification of β-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods

Cited by 6 publications

References 19 publications

Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans

Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans

Mass spectrometry methods for predicting antibiotic resistance

Hospital transmission of borderline oxacillin-resistant Staphylococcus aureus evaluated by whole-genome sequencing

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