Identification of xyloglucan endotransglucosylase/hydrolase genes (XTHs) and their expression in persimmon fruit as influenced by 1-methylcyclopropene and gibberellic acid during storage at ambient temperature

Zhu, Qingsen; Zhang, Zhengke; Rao, Jingping; Huber, Donald J.; Lv, Jingyi; Hou, Yali; Song, Kanghua

doi:10.1016/j.foodchem.2012.09.141

Cited by 36 publications

(29 citation statements)

References 25 publications

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“…The expression of these genes in fruit treated with 1-MCP was lower than the other treatments at harvest and throughout storage.This indicates that the expression of both XTH genes might be ethylene dependent. Similar results were observed by Zhu et al 2013 who found that astringent persimmon cv. Fupingjianshi treated with 1-MCP delayed the softening and suppressed the expression of XTH genes.…”

Section: Days At 20°c Relative Expressionsupporting

confidence: 90%

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Harvest and Postharvest Quality of Persimmon Fruit:physicochemical and Nutritional Aspects.

Borrás¹

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Section: Days At 20°c Relative Expressionsupporting

confidence: 90%

“…Members of the PG, XTH and EXP gene families have been shown to be involved in fruit softening processes of persimmons (Nakatsuka et al, 2011;De Souza et al, 2011;Zhu et al, 2013). Fig.…”

Section: Days At 20°c Relative Expressionmentioning

confidence: 99%

Harvest and Postharvest Quality of Persimmon Fruit:physicochemical and Nutritional Aspects.

Borrás¹

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“…Indeed, the depolymerization and solubilization of pectic and hemicellulosic polysaccharides in the cell wall have been demonstrated to be the major processes in fruit softening4. Xyloglucan, the major hemicellulose in the primary cell wall of dicotyledonous plants, comprises a network with cellulose microfibrils to provide strength to the cell wall56, with xyloglucan endotransglucosylases/hydrolases (XTHs) functioning in xyloglucan metabolism through xyloglucan endotransglycosylase (XET) and/or xyloglucan endohydrolase (XEH) activities78. XET activity results in the transfer of one xyloglucan molecule to another, whereas XEH activity hydrolyzes one xyloglucan molecule from the polymer910.…”

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confidence: 99%

“…Expression of XTH genes is regulated by developmental and environmental stimuli13, such as darkness, touch, cold/heat-shock1415, and by many hormones, such as ethylene16, abscisic acid (ABA)6, gibberellic acid (GA 3 )17, and auxins18.…”

mentioning

confidence: 99%

“…In our previous studies, seven XTH genes (DkXTH1–7) were amplified from persimmon, and these genes were proposed to be involved in fruit development, ripening or softening627. However, there is a lack of direct genetic evidence for these activities, and additional genes should be identified to provide a better understanding of the roles of specific genes in fruit.…”

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DkXTH8, a novel xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening

Han

Ban

et al. 2016

Sci Rep

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Fruit softening is mainly associated with cell wall structural modifications, and members of the xyloglucan endotransglucosylase/hydrolase (XTH) family are key enzymes involved in cleaving and re-joining xyloglucan in the cell wall. In this work, we isolated a new XTH gene, DkXTH8, from persimmon fruit. Transcriptional profiling revealed that DkXTH8 peaked during dramatic fruit softening, and expression of DkXTH8 was stimulated by propylene and abscisic acid but suppressed by gibberellic acid and 1-MCP. Transient expression assays in onion epidermal cells indicated direct localization of DkXTH8 to the cell wall via its signal peptide. When expressed in vitro, the recombinant DkXTH8 protein exhibited strict xyloglucan endotransglycosylase activity, whereas no xyloglucan endohydrolase activity was observed. Furthermore, overexpression of DkXTH8 resulted in increased leaf senescence coupled with higher electrolyte leakage in Arabidopsis and faster fruit ripening and softening rates in tomato. Most importantly, transgenic plants overexpressing DkXTH8 displayed more irregular and twisted cells due to cell wall restructuring, resulting in wider interstitial spaces with less compact cells. We suggest that DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants.

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1‐Methylcyclopropene (1‐MCP) retards the senescence ofPteridium aquilinum var. latiusculumby regulating the cellular energy status and membrane lipid metabolism

Zhang

et al. 2021

Food Science & Nutrition

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Pteridium aquilinum var. latiusculum (PA) is a popular fern species in the Athyriaceae family that is widely distributed in Northeast China.It is rich in nutrients and exhibits the abilities to promote the growth and development of children, improve body immunity, strengthen memory, and delay the aging of the brain (Yamada et al., 2007).Because of its classification as a natural food and its abundance of nutrients, it has attracted increasing numbers of consumers in recent years. In 2015, the consumption of wild vegetables in Heilongjiang Province of China reached approximately forty-one thousand tons.

show abstract

Identification of xyloglucan endotransglucosylase/hydrolase genes (XTHs) and their expression in persimmon fruit as influenced by 1-methylcyclopropene and gibberellic acid during storage at ambient temperature

Cited by 36 publications

References 25 publications

Harvest and Postharvest Quality of Persimmon Fruit:physicochemical and Nutritional Aspects.

Harvest and Postharvest Quality of Persimmon Fruit:physicochemical and Nutritional Aspects.

DkXTH8, a novel xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening

1‐Methylcyclopropene (1‐MCP) retards the senescence ofPteridium aquilinum var. latiusculumby regulating the cellular energy status and membrane lipid metabolism

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