Identification of Wolbachia Strains in Mosquito Disease Vectors

Osei-Poku, Jewelna; Han, Calvin; Mbogo, Charles M.; Jiggins, Francis M.

doi:10.1371/journal.pone.0049922

Cited by 34 publications

(29 citation statements)

References 47 publications

(80 reference statements)

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“…uniformis is similar to the 29% (5/19) reported in Kenyan populations 51 suggesting this species has variable prevalence rates in wild populations.…”

Section: Discussionsupporting

confidence: 80%

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Diverse novel residentWolbachiastrains in Culicine mosquitoes from Madagascar

Jeffries

Tantely

Raharimalala

et al. 2018

Preprint

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Wolbachia endosymbiotic bacteria are widespread throughout insect species and Wolbachia transinfected inAedes mosquito species has formed the basis for biocontrol programs as Wolbachia strains inhibit arboviral replication and can spread through populations. Resident strains in wild Culicine mosquito populations (the vectors of most arboviruses) requires further investigation given resident strains can also affect arboviral transmission. As Madagascar has a large diversity of both Culicine species and has had recent arboviral outbreaks, an entomology survey was undertaken, in five ecologically diverse sites, to determine the Wolbachia prevalence. We detected diverse novel resident Wolbachia strains within the Aedeomyia, Culex, Ficalbia, Mansonia and Uranotaenia genera. Wolbachia prevalence rates and strain characterisation through Sanger sequencing with multilocus sequence typing (MLST) and phylogenetic analysis revealed significant diversity and we detected co-infections with the environmentally acquired bacterial endosymbiont Asaia.Mosquitoes were screened for major arboviruses to investigate if any evidence could be provided for their potential role in transmission and we report the presence of Rift Valley fever virus in three Culex species:Culex tritaeniorhynchus, Culex antennatus and Culex decens. The implications of the presence of resident Wolbachia strains are discussed and how the discovery of novel strains can be utilized for applications in the development of biocontrol strategies.

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“…uniformis is similar to the 29% (5/19) reported in Kenyan populations 51 suggesting this species has variable prevalence rates in wild populations.…”

Section: Discussionsupporting

confidence: 80%

“…Ma. uniformis was also one of the four Wolbachia-infected mosquito species from populations in Kenya 51 and this provides the only comparative MLST and reports the requirement for nested PCR to amplify hcpA. Our Wolbachia prevalence rate of 29% (7/24) for Ma.…”

Section: Discussionmentioning

confidence: 82%

Diverse novel residentWolbachiastrains in Culicine mosquitoes from Madagascar

Jeffries

Tantely

Raharimalala

et al. 2018

Preprint

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“…This pattern is common in many other arthropods (Duron et al, 2008;Zug & Hammerstein, 2012), and it is usually associated with a weak manipulation of the host reproduction and/or imperfect maternal transmission (Engelstadter & Hurst, 2009). In general, our sampling effort was higher than in previous studies (n < 30) (Bourtzis et al, 2014;Osei-Poku et al, 2012), and this could explain why we found more infected species. Our statistical analysis showed that a sample size of 60 individuals per species is needed to quantify correct prevalence rates lower than 15%, with a probability of 95% ( Figure S3).…”

Section: Discussioncontrasting

confidence: 60%

Natural Wolbachia infections are common in the major malaria vectors in Central Africa

Ayala

Akone‐Ella

Rahola

et al. 2019

Evolutionary Applications

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During the last decade, the endosymbiont bacterium Wolbachia has emerged as a biological tool for vector disease control. However, for long time, it was believed that Wolbachia was absent in natural populations of Anopheles . The recent discovery that species within the Anopheles gambiae complex host Wolbachia in natural conditions has opened new opportunities for malaria control research in Africa. Here, we investigated the prevalence and diversity of Wolbachia infection in 25 African Anopheles species in Gabon (Central Africa). Our results revealed the presence of Wolbachia in 16 of these species, including the major malaria vectors in this area. The infection prevalence varied greatly among species, confirming that sample size is a key factor to detect the infection. Moreover, our sequencing and phylogenetic analyses showed the important diversity of Wolbachia strains that infect Anopheles . Co‐evolutionary analysis unveiled patterns of Wolbachia transmission within some Anopheles species, suggesting that past independent acquisition events were followed by co‐cladogenesis. The large diversity of Wolbachia strains that infect natural populations of Anopheles offers a promising opportunity to select suitable phenotypes for suppressing Plasmodium transmission and/or manipulating Anopheles reproduction, which in turn could be used to reduce the malaria burden in Africa.

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“…The negative samples confirmed to be truly negative. The negative samples were not excluded from the analysis as was done previously by Osei-Poku since the percentage was significantly higher than their estimation of 0.78% (Kittayapong et al, 2000;Osei-Poku et al, 2012). The 5.25% of the uninfected population may have been due to environmental factors.…”

Section: Discussionmentioning

confidence: 99%