Identification of Water-Soluble Selenium-Containing Proteins in Selenized Yeast by Size-Exclusion-Reversed-Phase HPLC/ICPMS Followed by MALDI-TOF and Electrospray Q-TOF Mass Spectrometry

Encinar, Jorge Ruiz; Ouerdane, Laurent; Buchmann, William; Tortajada, Jeanine; Łobiński, Ryszard; Szpunar, Joanna

doi:10.1021/ac034103m

Cited by 137 publications

(136 citation statements)

References 35 publications

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“…Fullerene standards were purchased from Sigma-Aldrich (Steinheim, Germany), selenopeptides were prepared from selenized yeast (Alltech, Lexington, KY, USA) according to [34]. Their molecular masses, structures, and sequences are listed in Table 3.…”

Section: Reagents and Materialsmentioning

confidence: 99%

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Buszewski¹,

Krupczyńska

Rychlicki

et al. 2006

J of Separation Science

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The chemical character, geometry, and architecture of chemically formed surface layers determine interactions between stationary phase, analyte, and mobile phase, and therefore the retention mechanisms (partitioning, adsorption, ion exchange, steric exclusion) of separated analytes. These interactions also depend on the structure and chemical character of the solutes and the composition of the mobile phase. High-molecular-weight fullerenes (C60 and C70) and water-soluble selenium-containing peptides (833 and 2607 Da) were used for the evaluation of laboratory-prepared octadecyl stationary phases with high and low coverage density before and after end-capping. The aim of this work was to study differences in surface coverage density and homogeneity and conformational changes of chemically bonded moieties and the influence of these parameters on the separation of mixtures of selenopeptides and fullerenes with significantly different molecular masses. A topographical model of the chemically modified stationary surface is presented.

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Section: Reagents and Materialsmentioning

confidence: 99%

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Buszewski¹,

Krupczyńska

Rychlicki

et al. 2006

J of Separation Science

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“…For example, SeMet, glutathione selenotrisulfide, selenite and selenate were detected in selenized yeast. 4,5,[31][32][33][34] Seleniumenriched plant foods (e.g., onion, garlic, shi-itake mushroom) and selenium-accumulating plants (e.g., Indian mustard) contain the selenoamino acids and their derivatives. [35][36][37][38] To date, the speciation analyses of biological samples have been mostly carried out using selenium-enriched plants.…”

Section: Non-proteinous Selenium Speciesmentioning

confidence: 99%

“…52) Modern mass spectrometric analyses, such as matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS) and electrospray ionization mass spectrometry (ESI-MS) are powerful techniques for the determination of the chemical structure of unknown selenium species according to its characteristic stable isotope pattern. [31][32][33][34] X-ray absorption near edge structure (XANES) spectroscopy is also useful for the detection of selenium species. This analytical technique is non-destructive and able to distinguish the chemical form of selenium.…”

Section: Non-proteinous Selenium Speciesmentioning

confidence: 99%

Selenium in Seafood Materials

Yoshida

Haratake

Fuchigami

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Research interests in studying the biochemical nature of selenium have increased and the importance of this element as an essential micronutrient in many organisms has been well recognized. Selenium occurs in proteins in the form of the 21st amino acid, selenocysteine (SeCys or Sec). In this review, we describe the speciation analysis of the fish-specific selenoproteins and non-proteinous selenium compounds, and the nutritional bioavailability of selenium from seafood materials. Selenium is essential to fish and shellfish. The selenoproteomes (sets of SeCys-containing proteins) of fish are greater in number than those of mammals (25 selenoproteins in humans); at 30-37 selenoproteins, the selenoproteomes of fish are among the largest known. The same core selenoprotein families are found in mammals and fish. In addition, fish have several species-specific selenoproteins [fish 15 kDa selenoprotein-like protein (Fep15), selenoprotein J and selenoprotein L] that are missing in mammals. Actually, not only proteinous selenium species like selenomethionine (SeMet) and SeCys derivatives, but also many non-proteinous organic ones were detected in fish and shellfish samples. Although the selenium contents in seafood are higher than in terrestrial foodstuffs, little is known about the chemical forms of organoselenium species in seafood. The nutritional bioavailability of selenium from seafood appears to be dependent on the fish and shellfish species and/or place where they are produced; some seafood gives rise to a high bioavailability of selenium, which is comparable to that of wheat and beef. Fish and shellfish materials are major dietary sources of selenium for the Japanese population (∼ 60% of daily intake). Seafood materials appear to contain nutritionally effective organoselenium compounds that have not yet been chemically identified.

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“…S-trace by the correction function (relative sensitivity S/P). The peaks in both traces were integrated and the 34 S-intensity was divided by the number of sulphur atoms (22) to get the relative protein abundance. Division of the 4.…”

Section: Characterisation Of Quantification Parameters and Data Evalumentioning

confidence: 99%

Quantification of Protein Phosphorylation by μLC-ICP-MS

Krüger¹,

Zinn

Lehmann

2009

Methods in Molecular Biology™

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Determination of the protein amount and of the extent of protein phosphorylation is crucial for a variety of research fields, but is not always straightforward. We describe the application of capillary LC-ICP-MS (liquid chromatography-inductively coupled plasma-mass spectrometry) for quantification of phospho-proteins and their phosphorylation degree. Element mass spectrometry is ideally suited for monitor ing and quantification of compounds with heteroelements such as phosphorus and sulphur, particularly because the ICP-MS response is virtually independent from the chemical form of the element. Determination of the phosphorylation stoichiometry, i.e. the relative abundance of the phosphorylated isoforms, can be assessed by the relative abundance of phosphorus compared with sulphur as a marker for the protein amount. Moreover, isotope dilution analysis by post-column addition of a 34S-Spike provides absolute protein quantification with exceptionally high accuracy. Phosphoprotein analysis by capillary LC-ICP-MS may be applied to isolated proteins or protein digests and may include separation of impurities by 1D-SDS-PAGE followed by enzymatic digestion. Alternatively, digestion of complex protein mixtures such as cellular protein extracts allows determination of global, tissue-specific phosphorylation degrees.

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Identification of Water-Soluble Selenium-Containing Proteins in Selenized Yeast by Size-Exclusion-Reversed-Phase HPLC/ICPMS Followed by MALDI-TOF and Electrospray Q-TOF Mass Spectrometry

Cited by 137 publications

References 35 publications

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Selenium in Seafood Materials

Quantification of Protein Phosphorylation by μLC-ICP-MS

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