Identification of virus-specific B-cell epitopes by convalescent plasma from COVID-19 patients

Zhao, Juan; Schank, Madison; Khanal, Sushant; Dang, Xindi; Cao, Dechao; Nguyen, Lam Nhat; Zhang, Yi; Wu, Xiao Y.; Adkins, James L.; Brueggeman, Justin; Zhang, Jinyu; Ning, Shunbin; Gazzar, Mohamed El; Moorman, Jonathan P.; Yao, Zhi Q.

doi:10.1016/j.molimm.2022.10.016

Cited by 4 publications

(3 citation statements)

References 28 publications

(30 reference statements)

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“…Identifying B-cell epitopes in viral proteins holds significant meaning for vaccine development, clinical diagnosis and antibody production. [ 27 , 28 , 29 ]. Clearly defining epitopes targeted by monoclonal antibodies will greatly contribute to understanding antigen structure and advancing antiviral therapies [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can impair immunological function, stunt growth and decrease egg production in avian flocks. The capsid protein (P27) is an attractive candidate for ALV diagnostics. In the present study, a new hybridoma cell (1F8) stably secreting an anti-P27 monoclonal antibody (mAb) was developed. The mAb exhibited a high affinity constant (Ka) of 8.65 × 106.0 L/mol, and it could be used for the detection of ALV-A/B/J/K strains. Moreover, a total of eight truncated recombinant proteins and five synthetic polypeptides were utilized for the identification of the B-cell epitopes present on P27. The results revealed that 218IIKYVLDRQK227 was the minimal epitope recognized by 1F8, which had never been reported before. Additionally, the epitopes could strongly react with different ALV subgroup’s specific positive serum and had a complete homology among all the ALV subgroups strains. Finally, a new sandwich ELISA method was created for the detection of ALV antigens, demonstrating increased sensitivity compared to a commercially available ELISA kit. These results offer essential knowledge for further characterizing the antigenic composition of ALV P27 and will facilitate the development of diagnostic reagents for ALV.

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Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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“…Bioinformatics is an interdisciplinary domain that concentrates on the organization, storage, and analysis of extensive volumes of data derived from biological experiments. It facilitates a comprehensive comprehension of biological functional mechanisms and molecular folding, rendering it an indispensable instrument for the advancement of vaccines [20][21][22]. However, despite its numerous advantages, the selection and design of protective immunogens against pathogens persist as a signi cant obstacle.…”

Section: Introductionmentioning

confidence: 99%

Display of Porcine Epidemic Diarrhea Virus Spike protein B-Cell Linear Epitope on Lactobacillus mucosae G01 S-layer Surface Induce a Robust Immunogenicity in Mice

Zhang,

Gou,

Shen

et al. 2024

Preprint

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The Porcine epidemic diarrhea virus (PEDV) presents a substantial risk to the domestic pig industry, resulting in extensive and fatal viral diarrhea among piglets. Recognizing the mucosal stimulation triggered by PEDV and harnessing the regulatory impact of lactobacilli on intestinal function, we have developed a lactobacillus based vaccine that is carefully designed to elicit a strong mucosal immune response. Through bioinformatics analysis, we examined PEDV S proteins to identify B-cell linear epitopes that meet the criteria of being non-toxic, soluble, antigenic, and capable of neutralizing the virus. In this study, a genetically modified strain of Lactobacillus mucosae G01 (L.mucosae G01) was created by utilizing the S layer protein (SLP) as a scaffold for surface presentation. Chimeric immunodominant epitopes with neutralizing activity were incorporated at various sites on SLP. The successful expression of SLP chimeric immunodominant epitope 1 on the surface of L.mucosae G01 was confirmed through indirect immunofluorescence and transmission electron microscopy, revealing the formation of a transparent membrane. The findings demonstrate that the oral administration of L.mucosae G01, which expresses the SLP chimeric immunodominant gene epitope1, induces the production of secreted IgA in the intestine and feces of mice. Additionally, there is an elevation in IgG levels in the serum. Moreover, the levels of cytokines IL-2, IL-4, IFN-γ, and IL-17 are significantly increased compared to the negative control group. These results suggest that L.mucosae G01 has the ability to deliver exogenous antigens and elicit a specific mucosal immune response against PEDV. This investigation presents new possibilities for immunoprophylaxis against PEDV-induced diarrhea.

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“…Bioinformatics is an interdisciplinary domain that concentrates on the organization, storage, and analysis of extensive volumes of data derived from biological experiments. It facilitates a comprehensive comprehension of biological functional mechanisms and molecular folding, rendering it an indispensable instrument for the advancement of vaccines [ 20 – 22 ]. However, despite its numerous advantages, the selection and design of protective immunogens against pathogens persist as a significant obstacle.…”

Section: Introductionmentioning

confidence: 99%

Display of porcine epidemic diarrhea virus spike protein B-cell linear epitope on Lactobacillus mucosae G01 S-layer surface induce a robust immunogenicity in mice

Zhang,

Gou,

Shen

et al. 2024

Microb Cell Fact

View full text Add to dashboard Cite

The Porcine epidemic diarrhea virus (PEDV) presents a substantial risk to the domestic pig industry, resulting in extensive and fatal viral diarrhea among piglets. Recognizing the mucosal stimulation triggered by PEDV and harnessing the regulatory impact of lactobacilli on intestinal function, we have developed a lactobacillus-based vaccine that is carefully designed to elicit a strong mucosal immune response. Through bioinformatics analysis, we examined PEDV S proteins to identify B-cell linear epitopes that meet the criteria of being non-toxic, soluble, antigenic, and capable of neutralizing the virus. In this study, a genetically modified strain of Lactobacillus mucosae G01 (L.mucosae G01) was created by utilizing the S layer protein (SLP) as a scaffold for surface presentation. Chimeric immunodominant epitopes with neutralizing activity were incorporated at various sites on SLP. The successful expression of SLP chimeric immunodominant epitope 1 on the surface of L.mucosae G01 was confirmed through indirect immunofluorescence and transmission electron microscopy, revealing the formation of a transparent membrane. The findings demonstrate that the oral administration of L.mucosae G01, which expresses the SLP chimeric immunodominant gene epitope1, induces the production of secreted IgA in the intestine and feces of mice. Additionally, there is an elevation in IgG levels in the serum. Moreover, the levels of cytokines IL-2, IL-4, IFN-γ, and IL-17 are significantly increased compared to the negative control group. These results suggest that L. mucosae G01 has the ability to deliver exogenous antigens and elicit a specific mucosal immune response against PEDV. This investigation presents new possibilities for immunoprophylaxis against PEDV-induced diarrhea.

show abstract

Identification of virus-specific B-cell epitopes by convalescent plasma from COVID-19 patients

Cited by 4 publications

References 28 publications

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Display of Porcine Epidemic Diarrhea Virus Spike protein B-Cell Linear Epitope on Lactobacillus mucosae G01 S-layer Surface Induce a Robust Immunogenicity in Mice

Display of porcine epidemic diarrhea virus spike protein B-cell linear epitope on Lactobacillus mucosae G01 S-layer surface induce a robust immunogenicity in mice

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