Identification of Vibrio species isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju Island, South Korea

Abstract

Olive flounder (Paralichthys olivaceus) is the major species developed for aquaculture in South Korea. Over the long history of olive flounder aquaculture, complex and diverse diseases have been a major problem, negatively impacting industrial production. Vibriosis is a prolific disease which continuously damages olive flounder aquaculture. A bacterial disease survey was performed from January to June 2017 on 20 olive flounder farms on Jeju Island. A total of 1710 fish were sampled, and bacteria from the external and internal organs of 560 fish were collected. Bacterial strains were identified using 16 s rRNA sequencing. Twenty-seven species and 184 strains of Vibrio were isolated during this survey, and phylogenetic analysis was performed. Bacterial isolates were investigated for the distribution of pathogenic and non-pathogenic species, as well as bacterial presence in tested organs was characterized. V. gigantis and V. scophthalmi were the dominant non-pathogenic and pathogenic strains isolated during this survey, respectively. This study provides data on specific Vibrio spp. isolated from cultured olive flounder in an effort to provide direction for future research and inform aquaculture management practices.

The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR method

“…None of V. parahaemolyticus found in the samples. The same ndings were reported and identi ed that Vibrio species was isolated from cultured olive ounder (Paralichthys olivaceus) in Jeju Island, South Korea and that there was no presence of V. parahaemolyticus, too (Sohn, Kim, Jin, & Lee, 2019). This was also reported by Hoang Tan Quang et al (2020) in which V. parahaemolyticus strain was a pathogen causing shrimp diseases, (Dao, Linh, & Khanh, 2014), (Khanh et al, 2019).…”

Indentification and Determination of Toxin Genes of Vibrio Strains Caused Hemorrhagic Disease on Red Drum (Sciaenops ocellatus) by PCR Method.

“…None of V. parahaemolyticus presence, the same reported of Sohn showed that identification of Vibrio species isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju Island, South Korea that none of V. parahaemolyticus Fig. 4 Phylogenetic tree of 30 strain bacteria Vibrio varieties collected based on the 16S rRNA region by the UPGMA method presence too (Sohn, Kim, Jin, & Lee, 2019). In which V. parahaemolyticus strain was a pathogen causing in shrimp disease, (Dao, Linh, & Khanh, 2014), (Khanh et al, 2019).…”

Identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR method

“…The digestive system has the most common Vibrio species (V. alginolyticus, V. azureus and V. uvialis). None of V. parahaemolyticus presented, the same reported and identi ed that Vibrio species was isolated from cultured olive ounder (Paralichthys olivaceus) in Jeju Island, South Korea that none of V. parahaemolyticus presence too (Sohn, Kim, Jin, & Lee, 2019). In which V. parahaemolyticus strain was a pathogen causing in shrimp disease, (Dao, Linh, & Khanh, 2014), (Khanh et al, 2019).…”