Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole ( Solea senegalensis , Kaup)

Medina, Alberto; Mancera, Juan Miguel; Martínez-Manzanares, Eduardo; Moriñigo, Miguel A.; Arijo, S.

doi:10.1016/j.fsi.2015.09.031

Cited by 12 publications

(3 citation statements)

References 25 publications

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“…It has been shown to induce IgG and IgA both in systemic and mucosal components in higher vertebrates [46] and hence is likely to induce mucosal and systemic immune responses in fish vaccinated by the oral route, using mechanisms similar to those seen in higher vertebrates. In fish, it has been shown to induce protective immunity against Vibrio harveyi in Senegalese sole ( Solea senegalensis , Kaup) [47], E. tarda in Japanese flounder ( Paralichthys olivaceus ) [48], Vibrio anguillarum in Asian sea bass ( Lates calcarifer ) [49], and A. hydrophila in carp [11]. As pointed out by Meenakshi et al [50], OMPs are only protective in the presence of adjuvants and, hence, it is likely that the higher protection induced by the NP-rOmpA vaccine was due to the adjuvant effect of the chitosan NPs used to deliver the rOmpA antigens in this study [51].…”

Section: Discussionmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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The use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. Biodegradable polymeric nanoparticles (NPs) have shown potential to serve as antigen delivery systems for oral vaccines. In this study the recombinant outer membrane protein A (rOmpA) of Edwardsiella tarda was encapsulated in chitosan NPs (NP-rOmpA) and used for oral vaccination of Labeo fimbriatus. The rOmpA purity was 85%, nanodiameter <500 nm, encapsulation efficiency 60.6%, zeta potential +19.05 mV, and there was an in vitro release of 49% of encapsulated antigen within 48 h post incubation in phosphate-buffered saline. Empty NPs and a non-formulated, inactivated whole cell E. tarda (IWC-ET) vaccine were used as controls. Post-vaccination antibody levels were significantly (p = 0.0458) higher in the NP-rOmpA vaccinated fish (Mean OD450 = 2.430) than in fish vaccinated with inactivated whole cell E. tarda (IWC-ET) vaccine (Mean OD450 = 1.735), which corresponded with post-challenge survival proportions (PCSP) of 73.3% and 48.28% for the NP-rOmpA and IWC-ET groups, respectively. Serum samples from NP-rOmpA-vaccinated fish had a higher inhibition rate for E. tarda growth on tryptic soy agar (TSA) than the IWC-ET group. There was no significant difference (p = 0.989) in PCSPs between fish vaccinated with empty NPs and the unvaccinated control fish, while serum from both groups showed no detectable antibodies against E. tarda. Overall, these data show that the NP-rOmpA vaccine produced higher antibody levels and had superior protection over the IWC-ET vaccine, showing that encapsulating OmpA in chitosan NPs confer improved protection against E. tarda mortality in L. fimbriatus. There is a need to elucidate the possible adjuvant effects of chitosan NPs and the immunological mechanisms of protective immunity induced by OMPs administered orally to fish.

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Section: Discussionmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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“…With the increase in breeding varieties in China, including economic varieties such as Cynoglossus semilaevis Gunther , Epinephelus coioides and Larimichthys crocea (Chen, Yao, Lin, & Chang, ; Pan et al, ), the host range of this bacterium has also gradually expanded. When the number of bacteria exceeds a certain threshold, a large‐scale outbreak will occur, with clinical symptoms including haemorrhagic sepsis, enteritis and ophthalmological diseases (Austin & Zhang, ; Medina, Mancera, Martínez‐Manzanares, Moriñigo, & Arijo, ).…”

Section: Introductionmentioning

confidence: 99%

Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

Guo

Xing

et al. 2019

Aquac Res

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Vibrio harveyi, as one of the main pathogens of vibriosis, has serious multidrug resistance, which has been a focus for domestic and foreign researchers. Vibrio harveyi hemolysin (VHH), an important virulence determinant of V. harveyi, causes cell necrosis and tissue damage by lysing cell membranes, which is essential for V. harveyi invasion of the host. The critical role of VHH in the virulence of V. harveyi renders it an ideal target for designing novel anti-virulence therapeutics. We found that honokiol, a natural compound, could act as a potent antagonist of VHH-mediated haemolysis at non-bacteriostatic concentrations. Honokiol can effectively inhibit the damage caused by VHH and V. harveyi both in vitro and in vivo. qRT-PCR and molecular docking analysis showed that although honokiol up-regulated the transcription level of vhh, it could block the active centre of VHH directly and forcefully, thereby reducing the catalytic activity of VHH. The results suggest that HNK could have great potential in the development of new aquaculture drugs. K E Y W O R D S anti-virulence, honokiol, VHH, Vibrio harveyi

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“…Therefore, the detection of immunogenic bacterial antigens is vital to design an antigen-based vaccine. Extracellular substances (ECP) and outer membrane have demonstrated a high specific immune response in immunized fish [8,9] and could be good candidates to select immunogenic proteins.…”

mentioning

confidence: 99%

Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens

Medina

García-Millán

Martínez-Manazanares

et al. 2019

Fish & Shellfish Immunology

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Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole ( Solea senegalensis , Kaup)

Cited by 12 publications

References 25 publications

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens

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