Identification of very small cancer stem cells expressing hallmarks of pluripotency in B16F10 melanoma cells and their reoccurrence in B16F10-derived clones

Câmara, Diana Aparecida Dias; Sá, Paulo Luiz de; Azevedo, Ricardo A.; Figueiredo, Carlos R.; Araldi, Rodrigo Pinheiro; Levy, Débora; Souza, Dener Madeiro de; Kerkis, Irina

doi:10.1016/j.yexcr.2020.111938

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…Previously, we demonstrated the feedback mechanisms in primitive stages of human leukemic, cancer, and non-cancer cultures [15,16] These results correspond with those obtained for human hematopoietic cell cultures by other researchers [17]. Observed correlations between the developmental stages of VSELSCs-VSCSCs-CSCs [11][12][13] led us to investigate the data for the presence of VSLSLCs, VSCSLCs, and VSELSCs in established cell lines. We took another look at the data we collected from HL-60 (Supplementary Figures S1 and S2) and conducted experiments on different human cell lines: AML (Figure 1), CML (Figure 2), ALL (Figure 3 and Figure S3), and Non-Small Cell Lung Cancer and Non-Cancer Embryo Kidney Cells (Figures 4 and 5).…”

Section: Discussionsupporting

confidence: 85%

“…The study of SCs involves investigations into their precursor developmental stages-VSELSCs [10]; however, these cellular stages are still controversial (Supplementary Text ST1). Interestingly, VSELSCs have also been found in the context of cancer [11][12][13][14]. Although there is information regarding CSLCs and LSLCs, reports addressing (V)SCSLCs, VSLSLCs, and VSELSCs in vitro are notably lacking [8,15,16].…”

Section: Introductionmentioning

confidence: 99%

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(Very) Small Stem-like Cells in Human Cell Cultures

Lica,

Pradhan

2023

Cancers

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Very Small Embryonic-like Stem Cells (VSELSCs) and Very Small Cancer Stem Cells (VSCSCs) are fields of intensive research. Although the presence in vitro of VSELSC and VSCSC cellular stage analogs appear probable, it has yet to be published. Utilizing established human cell cultures with varying populations of primitive cells, stained with CD markers specific to primitive stages, in addition to a fluorescent DNA dye, and following histochemical processing, we have developed a cytological method for detecting Very Small Leukemic Stem-like Cells (VSLSLCs), Very Small Cancer Stem-like Cells (VSCSLCs), and VSELSCs. This detection provides an opportunity to advance research in these areas.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

(Very) Small Stem-like Cells in Human Cell Cultures

Lica,

Pradhan

2023

Cancers

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“…This is because 2D cultures do not exhibit the cell-ECM interactions or other cell types found within the TME, such as immune and stromal cells. Furthermore, despite the evidence that 2D cultures from cancer cell lines have a certain degree of heterogeneity [274], this heterogeneity does not reflect the genotypic and phenotypic cell heterogeneity verified within the TME [273,275]. Further, the cancer cell monolayer cannot recapitulate the biochemical properties, composition, tissue architecture, cell behavior, and exosome-mediated intercellular communication verified within the TME [276][277][278][279].…”

Section: Future Prospects Of Cell-free Therapy For Cancer Treatment and Challenges To Be Overcomementioning

confidence: 94%

Exosomes in the Tumor Microenvironment: From Biology to Clinical Applications

Costa

Araldi

Vigerelli

et al. 2021

Cells

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Cancer is one of the most important health problems and the second leading cause of death worldwide. Despite the advances in oncology, cancer heterogeneity remains challenging to therapeutics. This is because the exosome-mediated crosstalk between cancer and non-cancer cells within the tumor microenvironment (TME) contributes to the acquisition of all hallmarks of cancer and leads to the formation of cancer stem cells (CSCs), which exhibit resistance to a range of anticancer drugs. Thus, this review aims to summarize the role of TME-derived exosomes in cancer biology and explore the clinical potential of mesenchymal stem-cell-derived exosomes as a cancer treatment, discussing future prospects of cell-free therapy for cancer treatment and challenges to be overcome.

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“…In addition, CSCs also express high levels of ATP-binding cassette (ABC) transporters, which increase the efflux of chemotherapeutics, limiting the efficacy of chemo agents. Furthermore, therapeutic resistance can be acquired as a consequence of molecular changes and clonal selection observed after an extended period of treatment [71][72][73][74][75].…”

Section: Role Of Inflammasomes In Lung Cancer Therapy Resistancementioning

confidence: 99%

Inflammasomes driven inflammation in lung cancer revisited: a short review

Costa

Souza

Teixeira³

et al. 2023

Explor Immunol

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Lung cancer is the leading cause of cancer-related deaths worldwide. The main risk factor for lung cancer is exposure to chemicals present in cigarettes and atmospheric pollutants, which, among other mechanisms, can increase the risk of cancer by inducing pulmonary inflammation. Among the complex features of inflammatory processes, the role of inflammasomes has attracted increasing attention due to their role in different stages of carcinogenesis. Inflammasomes are intracellular multiprotein complexes that when activated promote the maturation of interleukin-1beta (IL-1β) and IL-18, pro-inflammatory cytokines involved in the promotion, progression, epithelial-mesenchymal transition, metastasis, and resistance to therapy of lung cancer. In this way, this review summarizes the recent findings of inflammasome research in different stages of lung cancer, with a focus on non-small cell lung carcinoma (NSCLC), and highlights these multiprotein complexes as promising targets for cancer therapy.

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Identification of very small cancer stem cells expressing hallmarks of pluripotency in B16F10 melanoma cells and their reoccurrence in B16F10-derived clones

Cited by 8 publications

References 37 publications

(Very) Small Stem-like Cells in Human Cell Cultures

(Very) Small Stem-like Cells in Human Cell Cultures

Exosomes in the Tumor Microenvironment: From Biology to Clinical Applications

Inflammasomes driven inflammation in lung cancer revisited: a short review

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