Identification of Uterin Milk Protein (Utmt) Gene in Bali Cattle Using Direct Sequencing

Abstract

The objective of this research was to identify diversity of exon 5 UTMP gene fragment in Bali cattle using direct sequencing. The total 60 blood samples of Bali Cattle derived from BPTU Bali in Bali siland (20 heads), BPTU Serading in Sumbawa island (20 heads) and Village Breeding Center in Barru District South Sulawesi (20 heads) were used to evaluate their genetic diversity at exon 5 UTMP gene. The forward and reverse data sequences were analyzed using Bioedit program and alignment analysis was carried out using MEGA5 program. Meanwhile haplotype analysis was performed by DnaSPv5 program. The result showed that partial sequences in exon 5 UTMP gene had 16 haplotypes with the highest number of haplotypes ware found in VBC Barru district South Sulawesi (8 haplotypes). Moreover, the highest average of haplotype (h) and nucleotide (p) diversity were found in VBC Barru district South Sulawesi were 0.7949 and 0.0016, respectively. In addition, minisatellite insersion was found in exon 5 UTMP gene fragment on Bali cattle which are consist of 5'-CCA GTC ATG AAG AAG GCA GAG GTC GTC GTG CCG GCG AAA-3'. According to our results, haplotype and minisatellite variation in exon 5 UTMP gene fragment can be used as a candidate genetic marker specific for reproductive trait in the Bali cattle and for its strategy breeding program in the future.

The Uterine Milk Protein (UTMP) gene is associated with milk production traits in Iranian Holstein bulls

“…KHATIB et al (2007) investigated the expression pattern of UTMP in fetal and adult cattle tissues and studied its association with production traits in 2 independent Holstein populations: University of Wisconsin (UW) and Cooperative Dairy DNA repository (CDDR) identified an A/G SNP at position 1179 and an A/G SNP at position 1296 in bovine mRNA UTMP and SNP at position 1296 (in exon 4) was associated with a significant increase in productive life in 2 independent Holstein cattle populations, where the G allele expression at that position was associated with the longer productive life, while GG genotype of UTMP had a significant association with lower embryo survival rate (KHATIB et al, 2009), but in other study there was no significant association between observed SNPs and traits under study in buffalo (JEROME et al, 2015). JAKARIA et al (2016) observed haplotype and nucleotide diversity of UTMP gene in Bali cattle. Researchers showed that OPN, FGF2, ABCG2, STAT5A, UTMP and ABCG2 have considerable association with milk production and health traits in dairy cattle (MOUSAVIZADEH et al, 2013;SADEGHI et al, 2009).…”

“…The uterine milk protein gene (UTMP) was selected for this study because of its possible roles in health traits. Results of the other researchers showed the association of the UTMP region with milk production in dairy cattle as well (KHATIB et al, 2007;JAKARIA et al, 2016).…”