Identification of up-regulated genes in malignant glioma with subtraction hybridization: preliminary screening studies

Abstract

PurposeThis investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization.Materials and MethodsSubtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database.ResultsUsing semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.ConclusionTheses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.

Deregulation of LOXL1 and HTRA1 Gene Expression in Endometriosis

“…[30][31][32][33][34] Compared to other methodologies, like gene microarrays, it is stated that both have good efficacy to reveal sequences of genes with altered expression. 32 In 2000, Jiang and colleagues described some improvements in SH method, which included significant simplification in the process of cDNA subtraction, without loss of efficiency, and reduction in the experiment's costs.…”

“…Subtraction hybridization is one of the well known and potentially faster methods that has been used in genetics research for identifying differentially expressed genes associated with a particular disease. [30][31][32][33][34] Compared to other methodologies, like gene microarrays, it is stated that both have good efficacy to reveal sequences of genes with altered expression. 32 In 2000, Jiang and colleagues described some improvements in SH method, which included significant simplification in the process of cDNA subtraction, without loss of efficiency, and reduction in the experiment's costs.…”