“…Hesperopeuce mertensiana (Pinaceae) seed lipids s -Not found in any bacteria species -In total lipids of leaves of Salvia nemorosa t -In total lipids of sericea lespedeza and bermudagrass Dembitsky et al 1994 m Nikolova et al 2000 n Rütters et al 2001 o Poerschmann et al 2012 p Christie et al 1994 qTsydendambaev et al 2004 …”
Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 °C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C7-C30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
“…Hesperopeuce mertensiana (Pinaceae) seed lipids s -Not found in any bacteria species -In total lipids of leaves of Salvia nemorosa t -In total lipids of sericea lespedeza and bermudagrass Dembitsky et al 1994 m Nikolova et al 2000 n Rütters et al 2001 o Poerschmann et al 2012 p Christie et al 1994 qTsydendambaev et al 2004 …”
Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 °C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C7-C30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
“…The samples were injected in split mode (20:1), with an injection volume of 2 L. FAMEs were identified by comparison of their retention times with corresponding standards (Sigma-Aldrich) and by comparing their equivalent chain length values with data found in the literature. 28 Finally, presence of free phenolic acids was tested by an HPLC system consisting of a Synergi Hydro-RP stainless steel column (4.6 ϫ 250 mm, particle size 4 m, 80 Å; Phenomenex, Torrance, CA) using a Q-Grad pump (Watrex), SCU-450 gradient keypad controller (LabAlliance, State College, PA), Midas autosampler with 100-L sample loop (Spark, Emmen, The Netherlands), and UV6000LP photodiode array detector (Thermo Fisher Scientific Inc.). Mobile phases A (0.4% acetic acid in water) and B (0.4% acetic acid, 80% acetonitrile, and 19.6% water) were selected for gradient elution, which started at 0% B for 10 minutes followed up to 9% B from 35 to 55 minutes, 20% B in 65 minutes, 30% B in 80 minutes, 50% B in 90 minutes, and 100% B in 95 minutes, followed by maintenance at 105 minutes and then equilibration with the starting mobile phase A for 10 minutes.…”
Seed extracts from six species of the genus Nigella (Family Ranunculaceae)-Nigella arvensis, Nigella damascena, Nigella hispanica, Nigella nigellastrum, Nigella orientalis, and Nigella sativa-obtained by successive extraction with n-hexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution method as well as for anti-inflammatory properties by in vitro cyclooxygenase (COX)-1 and COX-2 assay. Chemical characterization of active extracts was carried out including free and fixed fatty acid analysis. Comparison of antimicrobial activity showed that N. arvensis chloroform extract was the most potent among all species tested, inhibiting Gram-positive bacterial and yeast strains with minimum inhibitory concentration (MIC) values ranging from 0.25 to 1 mg/mL. With the exception of selective inhibitory action of n-hexane extract of N. orientalis on growth of Bacteroides fragilis (MIC = 0.5 mg/mL), we observed no antimicrobial activity for other Nigella species. Anti-inflammatory screening revealed that N. sativa, N. orientalis, N. hispanica, N. arvensis n-hexane, and N. hispanica chloroform extracts had strong inhibitory activity (more than 80%) on COX-1 and N. orientalis, N. arvensis, and N. hispanica n-hexane extracts were most effective against COX-2, when the concentration of extracts was 100 microg/mL in both COX assays. In conclusion, N. arvensis, N. orientalis, and N. hispanica seeds, for the first time examined for antimicrobial and anti-inflammatory effects, revealed their significant activity in one or both assays.
“…Interestingly, we were able to detect an unusual fatty acid, that is, pentadecanoic acid (15:0), which was ostensibly reduced in transgenic plants. This type of saturated odd-numbered fatty acid, not commonly found in higher plants, has been found in alpine plants (Tsydendambaev et al 2004). The significance of this result is unknown.…”
In this work, we have genetically transformed tobacco (Nicotiana tabacum) plants with the peroxisome proliferator-activated receptor cDNA (xPPARalpha) from Xenopus laevis, which is a transcriptional factor involved in the peroxisomal proliferation and induction of fatty acid beta-oxidation in animal cells. Several transgenic lines were generated and one representative line (T) from the R2 generation was selected for further studies. Analysis of free fatty acids revealed that unsaturated fatty acids such as C16:2 and C16:3 were deficient in line T, whereas saturated fatty acids like C16:0, C18:0, and C20:0 were more abundant than in non-transformed plants. Acyl-CoA oxidase (ACOX) activity was assayed as a marker enzyme of beta-oxidation in crude leaf extracts and it was found that in line T there was a threefold increase in enzyme activity. We also found that the peroxisome population was increased and that catalase (CAT) activity was induced by clofibrate, a known activator of xPPARalpha protein, in leaves from line T. Taken together, these findings suggest that xPPARalpha is functional in plants and that its expression in tobacco leads to changes in general lipid metabolism and peroxisomal proliferation as reported in animal cells. Furthermore, it indicates that there is an endogenous ligand in tobacco cells able to activate xPPARalpha.
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