Identification of type II interferons and receptors in an osteoglossiform fish, the arapaima Arapaima gigas

Pang, An Ning; Chen, Shan Nan; Gan, Zhen; Li, Li; Li, Nan; Wang, Shuai; Sun, Zheng; Liu, Lan Hao; Sun, Yan; Xia, Song; Liu, Yang; Wang, Su; Nie, Pin

doi:10.1016/j.dci.2022.104589

Cited by 7 publications

(5 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The induced expression of ISGs is functionally recognized as signaling consequence of IFNs through their respective receptors, and type IV IFN is also verified to be able to induce the expression of ISGs ( Ouyang et al, 2011 ; Chen et al, 2013 , 2022 a, 2023 a; Gan et al, 2020 ; Pang et al, 2022 ). In the present study, the significant increase in the expression of IRF1, OASL, and STAT1 in kidney leukocytes following the stimulation of recombinant IFN-υ may imply the functional receptor composition on these leucocytes, and the increased expression of OAS and Viperin in HEK293T cells transfected with chimeric receptors, p3xFLAG-ApIFN-υR1+HsIFNLR1 and p3xFLAG-ApIL10RB+HsIL10RB, following incubation with recombinant IFN-υ protein, may confirm that IFN-υR1 and IL10RB are the compositional receptor subunits of type IV IFN in mallard as reported in fish and amphibian ( Chen et al, 2022a , 2023 a).…”

Section: Discussionmentioning

confidence: 94%

“…Kidney leukocytes were isolated as described previously ( Hennion and Hill, 2015 ; Pang et al, 2022 ). Briefly, kidney was dissected out from healthy mallard, and was washed twice in PBS containing 100 U/mL penicillin, 100 μg/mL streptomycin (PS), and 30 U/mL heparin sodium.…”

Section: Methodsmentioning

confidence: 99%

“…The construction of chimeric receptors is often used to characterize the binding relationship between a cytokine and its receptor. For example, such approach has been employed to characterize the binding of IL10 to IL10RB or CRFB4, IL22 to IL22R1 (IL-TIF-R1 or CRF2-9), IFNG to its receptor (IFNG-like and IFNG transduce downstream signaling via IFNGR1-1/IFNGR2-1, IFNGR1-2/IFNGR2-2, respectively), and IFNGR to Jak2 kinase ( Kotenko et al, 1996 , 1997 , 2001 ; Pang et al, 2022 ). Structurally, chimeric receptors contain the extracellular region of a specific receptor to be investigated in relation with its ligand binding feature, and a transmembrane region and also an intracellular region of a xenogeneic receptor with already characterized or reported signaling in a transfection system.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

IFN-υ and its receptor subunits, IFN-υR1 and IL10RB in mallard Anas platyrhynchos

Pang,

Chen,

Liu

et al. 2024

Poultry Science

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IFN-υ and its receptor subunits, IFN-υR1 and IL10RB in mallard Anas platyrhynchos

Pang,

Chen,

Liu

et al. 2024

Poultry Science

View full text Add to dashboard Cite

“…It has been proven that CRFB5 is a homolog of IFNAR1, while CRFB1 and CRFB2 are homologs of IFNAR2, as proven by sequence analysis and correlation experiments [19]. Some studies have found that virus infection or poly I:C stimulation could induce CRFB5 expression in some teleost fishes, including orange-spotted grouper (Epinephelus coioides), Asian Arowana (Scleropages formosus), Nile tilapia (Oreochromis niloticus), and grass carp [14,15,17,18]. For example, poly I:C stimulation caused the up-regulation of CRFB5 in the liver of tilapia and Asian arowana [14,20], as well as in grass carp kidney cells (CIK cell) [15], which demonstrated that CRFB5 was involved in antiviral immune responses in fish [20].…”

Section: Introductionmentioning

confidence: 99%

“…In teleost, the first type I IFN receptor was identified in the pufferfish (Tetraodontidae) cytokine receptor family B (CRFB) gene, which shares commonality with the cluster of IFN receptor genes found in mammals [12,13]. Different types of CRFB genes have been characterized in teleost fishes, such as CRFB1 and CRFB5 in zebrafish (Danio rerio), Atlantic salmon (Salmo salar), and grass carp (Ctenopharyngodon idella), CRFB2 and CRFB5 in tilapia (Oreochromis mossambicus) [14][15][16], and CRFB13 and CRFB17 in Arapaima (Arapaima gigas) [17]. Protein structure analysis showed that two of the fibronectin type-3 (FN3) domains were conserved in fish CRFB5, whereas four FN3 domains were present in human IFNAR1 [18].…”

Section: Introductionmentioning

confidence: 99%

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Wang,

Lin,

Wang

et al. 2023

Animals

View full text Add to dashboard Cite

IFNAR1, one of the type I IFN receptors, is crucial to mammalian host defense against viral invasion. However, largely unknown is the immunological role of the fish teleost protein IFNAR1, also known as CRFB5. We have successfully cloned the whole cDNA of the Japanese eel’s (Anguilla japonica) CRFB5a homolog, AjCRFB5a. The two fibronectin-3 domains and the transmembrane region (238–260 aa) of AjCRFB5a are normally present, and it shares a three-dimensional structure with zebrafish, Asian arowana, and humans. According to expression analyses, AjCRFB5a is highly expressed in all tissues found, particularly the liver and intestine. In vivo, Aeromonas hydrophila, LPS, and the viral mimic poly I:C all dramatically increased AjCRFB5a expression in the liver. Japanese eel liver cells were reported to express AjCRFB5a more strongly in vitro after being exposed to Aeromonas hydrophila or being stimulated with poly I: C. The membranes of Japanese eel liver cells contained EGFP-AjCRFB5a proteins, some of which were condensed, according to the results of fluorescence microscopy. Luciferase reporter assays showed that AjCRFB5a overexpression strongly increased the expression of immune-related genes in Japanese eel liver cells, such as IFN1, IFN2, IFN3, IFN4, IRF3, IRF5, and IRF7 of the type I IFN signaling pathway, as well as one of the essential antimicrobial peptides LEAP2, in addition to significantly inducing human IFN-promoter activities in HEK293 cells. Additionally, RNA interference (RNAi) data demonstrated that knocking down AjCRFB5a caused all eight of those genes to drastically lower their expression in Japanese eel liver cells, as well as to variable degrees in the kidney, spleen, liver, and intestine. Our findings together showed that AjCRFB5a participates in the host immune response to bacterial infection by inducing antimicrobial peptides mediated by LEAP2 and favorably modulates host antiviral immune responses by activating IRF3 and IRF7-driven type I IFN signaling pathways.

show abstract

Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway

Shibasaki,

Yabu,

Shiba

et al. 2024

FEBS Open Bio

View full text Add to dashboard Cite

Unlike mammals, fish express two type II interferons, IFNγ and fish‐specific IFNγ (IFNγ‐related or IFNγrel). We previously reported the presence of two IFNγrel genes, IFNγrel 1 and IFNγrel 2, which exhibit potent antiviral activity in the Ginbuna crucian carp, Carassius auratus langsdorfii. We also found that IFNγrel 1 increased allograft rejection; however, the IFNγrel 1 receptor(s) and signaling pathways underlying this process have not yet been elucidated. In this study, we examined the unique signaling mechanism of IFNγrel 1 and its receptors. The phosphorylation and transcriptional activation of STAT6 in response to recombinant Ginbuna IFNγrel 1 (rgIFNγrel 1) was observed in Ginbuna‐derived cells. Binding of rgIFNγrel 1 to Class II cytokine receptor family members (Crfbs), Crfb5 and Crfb17, which are also known as IFNAR1 and IFNGR1‐1, respectively, was detected by flow cytometry. Expression of the IFNγrel 1‐inducible antiviral gene, Isg15, was highest in Crfb5‐ and Crfb17‐overexpressing GTS9 cells. Dimerization of Crfb5 and Crfb17 was detected by chemical crosslinking. The results indicate that IFNγrel 1 activates Stat6 through an interaction with unique pairs of receptors, Crfb5 and Crfb17. Indeed, this cascade is distinct from not only that of IFNγ but also that of known IFNs in other vertebrates. IFNs may be classified by their receptor and signal transduction pathways. Taken together, IFNγrel 1 may be classified as a novel type of IFN family member in vertebrates. Our findings provide important information on interferon gene evolution in bony fish.

show abstract

Identification of type II interferons and receptors in an osteoglossiform fish, the arapaima Arapaima gigas

Cited by 7 publications

References 47 publications

IFN-υ and its receptor subunits, IFN-υR1 and IL10RB in mallard Anas platyrhynchos

IFN-υ and its receptor subunits, IFN-υR1 and IL10RB in mallard Anas platyrhynchos

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway

Contact Info

Product

Resources

About