Identification of Two Repressor Elements in the Mouse α2(I) Collagen Promoter

Miao, Kai; Potter, James J.; Anania, Frank A.; Rennie-Tankersley, Lynda; Mezey, Esteban

doi:10.1006/abbi.1998.0977

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…(Shrivastava and Calame, 1994). YY1 is a zinc-finger protein that belongs to the GLI-Kruppel gene family (Shi et al, 1991) and participates in the transcription of alpha 1 and alpha 2 collagen type I gene (Miao et al, 1999;Riquet et al, 2001) and genes with TATA-less promoters (Karantzoulis-Fegaras et al, 1999;Johansson et al, 1998). Indeed, a TATA-box was not found in the COL18A1 core promoter region, in agreement with the mouse TATA-less promoter (Liétard et al, 2000).…”

Section: Discussionsupporting

confidence: 68%

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Characterization of human Collagen XVIII promoter 2: Interaction of Sp1, Sp3 and YY1 with the regulatory region and a SNP that increases transcription in hepatocytes

et al. 2005

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Section: Discussionsupporting

confidence: 68%

“…HepG2 cells were obtained from the American Type Culture Collection (ATCC) and were maintained as described elsewhere (Miao et al, 1999). Twenty-four hours before transfection, cells were plated onto 12-well plates (1 Â10 5 /well).…”

Section: Cell Culture and Transient Transfection Assaysmentioning

confidence: 99%

Characterization of human Collagen XVIII promoter 2: Interaction of Sp1, Sp3 and YY1 with the regulatory region and a SNP that increases transcription in hepatocytes

et al. 2005

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“…For example, repression by YY1 of activation of the low density lipoprotein receptor gene mediated by the sterol regulatory element-binding protein is independent of YY1 binding directly to the low density lipoprotein receptor promoter, because YY1 interacts in solution with Sp1 and sterol regulatory element-binding protein (23). YY1 has been shown to bind to the upstream Col1a2 promoter region at -690/-613 bp and to modify Sp1 binding and repress Sp1-stimulated activity in hepatic stellate cells (39). Compared with the proximal Col1a2 promoter, which binds many of the same factors, the position of YY1 binding sites immediately upstream of the TATA box seems to be unique to the Col1a1 promoter, where our studies show activation rather than repression of transcription by YY1.…”

Section: Discussionmentioning

confidence: 99%

YY1 Is a Positive Regulator of Transcription of theCol1a1 Gene

Riquet

Tan

Choy

et al. 2001

Journal of Biological Chemistry

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Both cell-specific and ubiquitous transcription factors in fibroblasts have been identified as critical for expression of the Col1a1 gene, which encodes the ␣1 chain of type I collagen. Here, we report that Yin Yang 1 (YY1) binds to the Col1a1 promoter immediately upstream of the TATA box, and we examine the functional implications of YY1 binding for regulation of Col1a1 gene expression in BALBc/3T3 fibroblasts. The Col1a1 promoter region spanning base pairs (bp) -56 to -9 bound purified recombinant YY1 and the corresponding binding activity in nuclear extracts was supershifted using a YY1-specific antibody. Mutation of the TATA box to TgTA enhanced YY1 complex formation. Mutation analysis revealed two YY1 core binding sites at -40/-37 bp (YY1A) and, on the reverse strand, at -32/-29 bp (YY1B) immediately adjacent to the TATA box. In transfections using Col1a1-luciferase constructs, mutation of YY1A decreased activity completely (wild-type p350 (p350wt), -222/؉113 bp) or partially (p130wt, -84 bp/؉13 bp), whereas mutation of YY1B blocked the expression of both promoter constructs. Cotransfection with pCMV-YY1 increased p350wt and p130wt activities by as much as 10-fold, whereas antisense YY1 decreased constitutive expression and blocked the increased activity due to pCMV-YY1 overexpression. The mTgTA constructs were devoid of activity, arguing for a requirement for cognate binding of the TATA box-binding protein (TBP). Electrophoretic mobility shift assays performed under conditions permitting TBP binding showed that recombinant TBP/TFIID and YY1 could bind to the -56/-9 bp fragment and that YY1B was the preferred site for YY1 binding. Our results indicate that YY1 binds to the Col1a1 proximal promoter and functions as a positive regulator of constitutive activity in fibroblasts. Although YY1 is not sufficient for transcriptional initiation, it is a required component of the transcription machinery in this promoter.

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“…Nonetheless, overexpression of AP-2 by transfection did not further stimulate this region of the TRIII promoter. This suggests that the amount of endogenous AP-2 may be sufficient for TRIII expression or that complex interactions between Sp1 and AP-2 may govern overall TRIII expression (44). Other oligonucleotide probes from this region also formed several gel shift complexes, but their identity is not yet known.…”

Section: Table IImentioning

confidence: 99%

Cloning the Promoter for Transforming Growth Factor-β Type III Receptor

Ji¹,

Chen²,

McCarthy

et al. 1999

Journal of Biological Chemistry

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Transforming growth factor-␤ binds to three high affinity cell surface molecules that directly or indirectly regulate its biological effects. The type III receptor (TRIII) is a proteoglycan that lacks significant intracellular signaling or enzymatic motifs but may facilitate transforming growth factor-␤ binding to other receptors, stabilize multimeric receptor complexes, or segregate growth factor from activating receptors. Because various agents or events that regulate osteoblast function rapidly modulate TRIII expression, we cloned the 5 region of the rat TRIII gene to assess possible control elements. DNA fragments from this region directed high reporter gene expression in osteoblasts. Sequencing showed no consensus TATA or CCAAT boxes, whereas several nuclear factors binding sequences within the 3 region of the promoter co-mapped with multiple transcription initiation sites, DNase I footprints, gel mobility shift analysis, or loss of activity by deletion or mutation. An upstream enhancer was evident 5 proximal to nucleotide ؊979, and a silencer region occurred between nucleotides ؊2014 and ؊2194. Glucocorticoid sensitivity mapped between nucleotides ؊687 and ؊253, whereas bone morphogenetic protein 2 sensitivity comapped within the silencer region. Thus, the TRIII promoter contains cooperative basal elements and dispersed growth factor-and hormone-sensitive regulatory regions that can control TRIII expression by osteoblasts. Several cell surface receptors for transforming growth factor-␤ (TGF-␤)1 are now known. Type I and type II receptors (TRI and TRII) have intracellular kinase domains responsible for heterologous receptor activation or downstream signal transduction (1-4). The type III receptor (TRIII), a membraneanchored proteoglycan also termed betaglycan, is thought to have a biological function distinct from TRI and TRII (5-7). The rat TRIII gene encodes a 91.6-kDa protein core that is modified by approximately 10 kDa of N-linked glycosyl residues and 150 -200 kDa of heparan and chondroitin sulfate side chains.TRIII has a relatively short, 43-amino acid cytoplasmic domain that lacks commonly recognized protein docking or kinase like motifs but is enriched with serines and threonines to approximately 42% (5, 6).TRIII is prevalent on many fetal cells, where it can be the most abundant TGF-␤-binding site. All TGF-␤ isoforms bind to TRIII with comparably high affinity, although this is about 3-5-fold less than that for TRI and TRII (7). Certain cells lack TRIII but maintain TGF-␤ sensitivity. Nonetheless, TRIII may attract and enhance TGF-␤ binding to TRII and form a more stable ligand-receptor complex (6). On certain cell types this appears more pronounced or limited to the TGF-␤2 isoform (8, 9). Moreover, disproportionately high levels of TRIII may sequester and possibly limit its binding to signaling receptor complexes (10 -12).The relative amount of TRIII is thought to vary with development, with differentiation, or in a tissue-specific manner. For example, TRIII levels are prevalent on less different...

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Identification of Two Repressor Elements in the Mouse α2(I) Collagen Promoter

Cited by 12 publications

References 36 publications

Characterization of human Collagen XVIII promoter 2: Interaction of Sp1, Sp3 and YY1 with the regulatory region and a SNP that increases transcription in hepatocytes

Characterization of human Collagen XVIII promoter 2: Interaction of Sp1, Sp3 and YY1 with the regulatory region and a SNP that increases transcription in hepatocytes

YY1 Is a Positive Regulator of Transcription of theCol1a1 Gene

Cloning the Promoter for Transforming Growth Factor-β Type III Receptor

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