Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.)

Kotresh, H.; Fakrudin, B.; Punnuri, Somashekhar; Rajkumar, B.; Thudi, Mahendar; Paramesh, H.; Lohithswa, H.; Kuruvinashetti, M. S.

doi:10.1007/s10681-005-9059-2

Cited by 50 publications

(28 citation statements)

References 12 publications

(9 reference statements)

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“…In F3 generation, the segregation pattern of 78 F3 families for plume moth reaction was good fit for 1 (18 non segregating resistant): 2 (46 segregating): 1 (14 non segregating susceptible) ratio (χ 2 = 2.923, p-value = 0.403), evidencing monogenic segregation pattern and single dominant gene action, as reported for pod borer resistance (Verulkar et al, 1997;Aruna et al, 2005;Mishra et al, 2013) and Fusarium wilt resistance (Kotresh et al, 2006) in pigeon-pea. This monogenic segregation for plume moth resistance was further established by 1:1 segregation pattern at BC1F1 (χ 2 = 0.332, p-value = 0.564) lines.…”

Section: Inheritance Of Plume Moth Resistancesupporting

confidence: 63%

“…Linkage analysis using segregation data showed PPM1 locus was linked to OPA09910 at 12.7 cM (recombination fraction 0.121). Only few reports pertaining to linkage association of RAPD markers with Fusarium wilt resistance (Kotresh et al, 2006), plant type (Dhanasekar et al, 2010), and pod borer resistance were available in pigeon-pea.…”

Section: Inheritance Of Plume Moth Resistancementioning

confidence: 99%

“…The random amplified polymorphic DNA (RAPD) markers (Williams et al, 1990) coupled with bulked segregant analysis (BSA; Michelmore et al, 1991) has been used for molecular tagging and mapping of genes controlling agronomically important traits in various crops including pigeon-pea (Kotresh et al, 2006;Ganapathy et al, 2009;Dhanasekar et al, 2010;Mishra et al, 2013). Recent days, RAPD markers are not favored in breeding endeavors due to its inconsistency in amplification across the laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

Mishra

Nag

Panigrahi

2015

Chilean J. Agric. Res.

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The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886) derived from pigeonpea (Cajanus scarabaeoides (L.) Thouars) accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families derived from an interspecific cross C. cajan (L.) Huth ('ICP-26') × C. scarabaeoides (accession ICPW-94) revealed monogenic gene action for resistance to plume moth, and the dominant control by single locus or cluster of tightly linked alleles. Bulked segregant analysis (BSA) of 116 F2 progenies by using 143 parental polymorphic RAPD primers could identify a fragment OPA09910 associated with plume moth resistance in coupling phase of linkage. Further single plant analysis of the 116 F2 mapping population revealed OPA09910 was linked to PPM1 locus conferring host resistance to plume moth with recombination fraction (rf) value of 0.125 (12.7 cM of Kosambi function). The resistance specific fragment OPA09910 was cloned, sequenced and converted into a sequence characterized amplified region (SCAR) marker, SCOPA09942, which was also closely associated (10.3 cM) with the locus PPM1 with rf value 0.102. BLAST analysis with pigeon-pea genome sequence also confirmed its occurrence in CcLG02 (Scafseq.LG_V5.0fa) and contig 01597 (AFSP01.fsa1). This SCAR marker showed reasonable screening efficiency in the F2, F3, and BC1F1 lines, thus it can be used as genetic handle in marker-assisted introgression of the genomic fragment conferring plume moth resistance and screening of breeding lines in pigeon-pea.

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Section: Inheritance Of Plume Moth Resistancesupporting

confidence: 63%

Section: Inheritance Of Plume Moth Resistancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

Mishra

Nag

Panigrahi

2015

Chilean J. Agric. Res.

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“…(Kassem et al, 2006) For instance, BSA was used for mapping of Fusarium wilt (FW) resistance with RAPD markers in the case of pigeonpea (Kotresh et al, 2006). On the other hand, in order to understand the complex nature of drought tolerance in chickpea, precise phenotypic data for 20 different root traits was analyzed using a QTL mapping approach.…”

Section: Comprehensive Genetic Maps Andmentioning

confidence: 99%

Translational Genomics in Agriculture: Some Examples in Grain Legumes

Varshney

Kudapa

Pazhamala

et al. 2014

Critical Reviews in Plant Sciences

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“…RAPD markers have also been widely used in the grain legumes for the depiction of genetic relationships among cultivars, among wild allies, or between cultivars and wild allies [16][17][18][19][20][21][22][23][24][25]. In Cajanus species, RAPD markers have been employed for genetic fingerprinting, genetic diversity assessment and gene tagging [26][27][28]. We report here on the utilization of RAPD markers to elucidate and validate the genetic relationships between the cultivated C. cajan and ten allied species of Cajanus, being the potential donor source of genes during inter-specific breeding programmes.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of Genetic Relationships in the Genus Cajanus Using Random Amplified Polymorphic DNA Marker Analysis

Mishra¹

2013

J Phylogen Evolution Biol

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In this present investigation, a total of 27 RAPD primers were used to elucidate the genetic relationships between cultivated Cajanus cajan cultivars and 10 allied species of primary, secondary and tertiary gene pool, including C. cajanifolius, C. scarabaeoides, C. platycarpus, C. albicans, C. volubilis, C. sericeus, C. acutifolius, C. lineatus, C. lanceolatus and C. reticulates. All primers showed polymorphism at species level and produced 215 unequivocal polymorphic bands, with an average of 7.96 bands per primer. These polymorphic primers exhibited variation with regard to average band informativeness, resolving power, and showed high polymorphism information content value. No single primer was able to distinguish between all the two cultivars of C. cajan and ten allied species of Cajanus, but several species specific amplified fragments were observed. The pair wise Jaccard's similarity coefficient values revealed high level of inter-specific genetic variation in the genus Cajanus. Cluster analysis exhibited the grouping of two C. cajan accessions with C. cajanifolius in one cluster, while except C. platycarpus, all the nine wild Cajanus species belonging to the secondary and tertiary gene pool form another cluster. The present analysis more or less agreed with the sectional classification of the genus Cajanus, and it has been hypothesized that cultivated pigeonpea has evolved through multi-genomic interaction through C. cajanifolius, and it has experienced minor genomic reorganization during its divergence. Again, identification of species specific amplification pattern substantiated the utility of RAPD markers on selection of suitable species to transfer the desirable trait into cultivated C. cajan, through marker aided breeding for its genetic augmentation, and also for the effective management of genetic resources of C. cajan.

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Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.)

Cited by 50 publications

References 12 publications

Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

Development of dominant sequence characterized amplified region (SCAR) marker linked with plume moth (Exelastis atomosa Walsingham 1886) resistance in pigeon-pea

Translational Genomics in Agriculture: Some Examples in Grain Legumes

Elucidation of Genetic Relationships in the Genus Cajanus Using Random Amplified Polymorphic DNA Marker Analysis

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