Identification of two polyketide synthase gene clusters on the linear plasmid pSLA2-L in Streptomyces rochei

Suwa, Masanori; Sugino, Hiroyuki; Sasaoka, Akiko; Mori, Eijiro; Fujii, Shingo; Shinkawa, Hidenori; Nimi, Osamu; Kinashi, Haruyasu

doi:10.1016/s0378-1119(00)00060-3

Cited by 38 publications

(34 citation statements)

References 25 publications

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“…S. griseus strains were reciprocally cultured at 289 C in a 500-ml Sakaguchi ‰ask containing 100 ml of GMP medium and total DNAs were isolated as described by Suwa et al 17) Gel samples for PFGE were prepared by the mycelium method as described previously, 2,18) digested in gels, and separated by contour-clamped homogeneous electricˆelds (CHEF). 19) CHEF was done in 0.5×TBE buŠer using 1.0z agarose at 159 C. E. coli strains were cultured at 379 C in LB medium, and cosmid and plasmid DNAs were extracted as described by Sambrook et al 20) Southern hybridization.…”

Section: Mutation Of S Griseus 2247mentioning

confidence: 99%

Chromosomal Circularization inStreptomyces griseusby Nonhomologous Recombination of Deletion Ends

Inoue

Higashiyama

Uchida

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Section: Mutation Of S Griseus 2247mentioning

confidence: 99%

Chromosomal Circularization inStreptomyces griseusby Nonhomologous Recombination of Deletion Ends

Inoue

Higashiyama

Uchida

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…1, compound 1) and carries three large linear plasmids, pSLA2-L, -M, and -S. Correlation between the antibiotic-producing ability and the plasmid profiles of mutants derived from strain 7434AN4 suggested that the largest plasmid, pSLA2-L, is involved in the production of both antibiotics (14). This idea was further supported by identification on pSLA2-L of a region homologous to eryAI, a typical modular PKS gene for erythromycin (EM), and an experiment in which it was disrupted (15,29). Finally, we determined the complete nucleotide sequence of pSLA2-L (210,614 bp) and identified 143 open reading frames on it (19).…”

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confidence: 94%

Analysis of the Loading and Hydroxylation Steps in Lankamycin Biosynthesis in Streptomyces rochei

Arakawa

Kodama

Tatsuno

et al. 2006

Antimicrob Agents Chemother

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The biosynthetic gene cluster of lankamycin (LM), a 14-member macrolide antibiotic, is encoded on the 210-kb linear plasmid pSLA2-L in Streptomyces rochei 7434AN4. LM contains a 3-hydroxy-2-butyl group at the C-13 position, which is different from an ethyl group in erythromycin. The following two possibilities could be considered for the origin of this starter moiety of LM biosynthesis: (i) an extra module exists in the biosynthetic gene cluster and loads an additional acetate molecule, or (ii) 3-hydroxy-2-butyrate or its equivalent is loaded and incorporated as a starter. The former possibility was eliminated by the complete sequencing of pSLA2-L, which showed no extra module. On the other hand, the latter was confirmed by incorporation of deuterium in [3-2 H]DL-isoleucine into the C-14 position of LM. The timing of hydroxylation reactions at the C-15 and C-8 positions of LM was studied by constructing disruptants of two P450 hydroxylase genes, lkmF (orf26) and lkmK (orf37). The lkmF disruptant produced 8-deoxylankamycin, while the lkmK disruptant produced both 15-deoxylankamycin and 8,15-dideoxylankamycin. These results clearly showed that LkmF is a C-8 hydroxylase and LkmK is a C-15 hydroxylase in LM biosynthesis and in addition suggested the order of hydroxylation steps; namely, hydroxylation may occur at first at C-15 by LkmK and then at C-8 by LkmF.

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“…A number of bacteria within the order Actinomycetales contain linear plasmids which encode catabolic traits (Dabrock et al, 1994;Kosono et al, 1997;Shimizu et al, 2001;Coleman & Spain, 2003;König et al, 2004), hydrogen autotrophy (Kalkus et al, 1990), determinants of virulence (Crespi et al, 1992), heavy metal resistance (Dabrock et al, 1994;Ravel et al, 1998;Stecker et al, 2003) or the production of secondary metabolites (Kinashi et al, 1987;Suwa et al, 2000). Linear plasmids have been described to occur in species of Streptomyces (Hayakawa et al, 1979;Kinashi et al, 1987;Chen et al, 1993;Kinoshita-Iramina et al, 1997;Suwa et al, 2000;Spatz et al, 2002), Rhodococcus (Crespi et al, 1992;Kalkus et al, 1990;Kosono et al, 1997;Shimizu et al, 2001;Stecker et al, 2003;König et al, 2004), Mycobacterium (Picardeau & Vincent, 1997, 1998Le Dantec et al, 2001;Coleman & Spain, 2003), Planobispora (Polo et al, 1998) and Clavibacter (Brown et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Linear plasmids have been described to occur in species of Streptomyces (Hayakawa et al, 1979;Kinashi et al, 1987;Chen et al, 1993;Kinoshita-Iramina et al, 1997;Suwa et al, 2000;Spatz et al, 2002), Rhodococcus (Crespi et al, 1992;Kalkus et al, 1990;Kosono et al, 1997;Shimizu et al, 2001;Stecker et al, 2003;König et al, 2004), Mycobacterium (Picardeau & Vincent, 1997, 1998Le Dantec et al, 2001;Coleman & Spain, 2003), Planobispora (Polo et al, 1998) and Clavibacter (Brown et al, 2002). The size of these linear replicons ranges from 11?7 to more than 600 kb, and most of them have been shown to be capable of conjugative transfer (Meinhardt et al, 1997;Picardeau & Vincent, 1998;Ravel et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…We have characterized the gene cluster encoding this 'upper part' of the degradation pathway (Parschat et al, 2003); however, the genes of anthranilate utilization by strain Rü61a have not been identified so far. A number of bacteria within the order Actinomycetales contain linear plasmids which encode catabolic traits (Dabrock et al, 1994;Kosono et al, 1997;Shimizu et al, 2001;Coleman & Spain, 2003; König et al, 2004), hydrogen autotrophy (Kalkus et al, 1990), determinants of virulence (Crespi et al, 1992), heavy metal resistance (Dabrock et al, 1994;Ravel et al, 1998;Stecker et al, 2003) or the production of secondary metabolites (Kinashi et al, 1987;Suwa et al, 2000). Linear plasmids have been described to occur in species of Based on the observation that mutants unable to grow on quinaldine occur spontaneously when A. nitroguajacolicus…”

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Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate

et al. 2005

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Arthrobacter nitroguajacolicus Rü 61a, which utilizes quinaldine as sole source of carbon and energy, was shown to contain a conjugative linear plasmid of approximately 110 kb, named pAL1. It exhibits similarities with other linear plasmids from Actinomycetales in that it has proteins covalently attached to its 59 ends. Southern hybridization with probes for the genes encoding quinaldine 4-oxidase and N-acetylanthranilate amidase indicated that pAL1 contains the gene cluster encoding the degradation of quinaldine to anthranilate. A mutant of strain Rü 61a that had lost pAL1 indeed could not convert quinaldine, but was still able to grow on anthranilate. Conjugative transfer of pAL1 to the plasmid-less mutant of strain Rü 61a and to Arthrobacter nicotinovorans DSM 420 (pAO1) occurred at frequencies of 5?4610 "4 and 2?0610per recipient, respectively, and conferred the ability to utilize quinaldine. Five other quinaldine-degrading Gram-positive strains were isolated from soil samples; 16S rDNA sequence analysis suggested the closest relationship to different Arthrobacter species. Except for strain K2-29, all isolates contained a pAL1-like linear plasmid carrying genes encoding quinaldine conversion. A 478 bp fragment that on pAL1 represents an intergenic region showed 100 % sequence identity in all isolates harbouring a pAL1-like plasmid, suggesting horizontal dissemination of the linear plasmid among the genus Arthrobacter. INTRODUCTIONBacteria of the genus Arthrobacter are considered to be ubiquitous in soil and have been found to be among the predominant members of culturable communities from several terrestrial subsurface environments (Crocker et al., 2000). Among the explanations advanced for their ubiquity or even predominance in soil are their resistance to desiccation and nutrient depletion, and their nutritional versatility. Arthrobacter spp. utilize a wide and varied range of natural as well as xenobiotic compounds and thus may play a significant role in the mineralization of organic matter in the environment (Cacciari & Lippi, 1987).Arthrobacter nitroguajacolicus strain Rü61a (formerly assigned to the species Arthrobacter ilicis) utilizes quinaldine (2-methylquinoline), a constituent of coal tar, as sole source of carbon and energy (Hund et al., 1990). Degradation via the anthranilate pathway ( Fig. 1) is initiated by the oxidation of quinaldine to 1H-4-oxoquinaldine, catalysed by quinaldine 4-oxidase (Qox). 1H-4-oxoquinaldine 3-monooxygenase subsequently generates 1H-3-hydroxy-4-oxoquinaldine, which undergoes 2,4-dioxygenolytic ring cleavage to form carbon monoxide and N-acetylanthranilate. This unusual mode of ring cleavage is catalysed by a cofactor-less 2,4-dioxygenase that does not share any similarity with aromatic ring cleavage dioxygenases, but seems to belong to the a/b-hydrolase fold superfamily of proteins (Fetzner, 2002). In the next step, an amidase (Amq) catalyses the hydrolysis of N-acetylanthranilate to anthranilate. We have characterized the gene cluster encoding this 'upper part' of th...

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Identification of two polyketide synthase gene clusters on the linear plasmid pSLA2-L in Streptomyces rochei

Cited by 38 publications

References 25 publications

Chromosomal Circularization inStreptomyces griseusby Nonhomologous Recombination of Deletion Ends

Chromosomal Circularization inStreptomyces griseusby Nonhomologous Recombination of Deletion Ends

Analysis of the Loading and Hydroxylation Steps in Lankamycin Biosynthesis in Streptomyces rochei

Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate

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