Identification of two novel C-type lectins involved in immune defense against white spot syndrome virus and Vibrio parahaemolyticus from Marsupenaeus japonicus

Zheng, Jinbin; Mao, Yong; Su, Yongquan; Wang, Jun

doi:10.1016/j.aquaculture.2019.734797

Cited by 14 publications

(5 citation statements)

References 48 publications

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“…The VP24-truncated isolate of white spot syndrome virus possessed lower infectivity in viral infection [28] . Furthermore, several previous studies suggested that VP24 interacted with several pathogen-associated molecular pattern during WSSV infection [29] , [30] , [31] . Herein, we discovered two highly homologous lysozyme from crayfish.…”

Section: Discussionmentioning

confidence: 94%

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Yin

Li-Chao

et al. 2021

Fish and Shellfish Immunology Reports

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Section: Discussionmentioning

confidence: 94%

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Yin

Li-Chao

et al. 2021

Fish and Shellfish Immunology Reports

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“…Since abuse of antibiotics pose serious threats to the ecological environment and human health ( 8 , 9 ), mining bioactive molecules to develop novel antimicrobial agents has attracted increasing attention of researchers. CTLs, which show high diversity in invertebrates, are promising candidates for antimicrobial agents for their antibacterial and antiviral activities ( 14 , 15 , 24 , 35 , 62 ). In this study, based on the full-length transcriptome analysis, the bacterial binding and antibacterial activities of four PtCTLs were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The rPtCTL-1 and rPtCTL-3 possessed the broadest spectrum of bacterial binding activity against the tested bacteria, including V. alginolyticus , V. parahaemolyticus and S. aureus , which was similar to previous studies ( 70 ). In addition to act as PRRs, CTLs also exhibit antibacterial activities ( 14 , 24 , 62 , 64 ). Corresponding to the bacterial binding activities, antibacterial assays showed that rPtCTL-1 and rPtCTL-3 possessed the widest spectrum of antibacterial activity against the tested bacteria, which was similar with a CTL isolated from P .…”

Section: Discussionmentioning

confidence: 99%

“…The rPtCTL-2 only displayed bacterial binding and antibacterial activities against V. alginolyticus against the tested bacteria, whereas no bacterial binding and antibacterial activities of rPtCTL-4 was observed against the tested bacteria, which was different to previous studies ( 70 – 72 ). Differences in bacterial binding and antibacterial activities of the four rPtCTLs might due to the mutation of the carbohydrate binding motif ( 24 , 73 ). Additionally, previous reports showed that the CRD recognized subtle differences in the arrangement and branching of the carbohydrate residues, which may result in specific sets of carbohydrate recognition profiles for CRD ( 74 ).…”

Section: Discussionmentioning

confidence: 99%

“…The purification of recombinant proteins was conducted using the high affinity Ni-NTA resin (GenScript, China) according to the manufacturer’s protocols. The purity of the recombinant proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie Blue staining, and the recombinant proteins were further confirmed by western blot analysis as previously described ( 24 ). The concentrations of recombinant proteins were examined using BCA Protein Assay Kit (Beyotimie, China).…”

Section: Methodsmentioning

confidence: 99%

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Full-Length Transcriptome Analysis Provides New Insights Into the Diversity of Immune-Related Genes in Portunus trituberculatus

Zhang

Bai

et al. 2022

Front. Immunol.

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Generally, invertebrates were thought to solely rely on their non-specific innate immune system to fight against invading microorganisms. However, increasing studies have implied that the innate immune response of invertebrates displayed diversity and specificity owing to the hyper-variable immune molecules in organisms. In order to get an insight into the diversity of immune-related genes in Portunus trituberculatus, a full-length transcriptome analysis of several immune-related tissues (hemocytes, hepatopancreas and gills) in P. trituberculatus was performed and the diversity of several immune-related genes was analyzed. The full-length transcriptome analysis of P. trituberculatus was conducted using a combination of SMRT long-read sequencing and Illumina short-read sequencing. A total of 17,433 nonredundant full-length transcripts with average length of 2,271 bp and N50 length of 2,841 bp were obtained, among which 13,978 (80.18%) transcripts were annotated. Moreover, numerous transcript variants of various immune-related genes were identified, including pattern recognition receptors, antimicrobial peptides, heat shock proteins (HSPs), antioxidant enzymes and vital molecules in prophenoloxidase (proPO)-activating system. Based on the full-length transcriptome analysis, open reading frames (ORFs) of four C-type lectins (CTLs) were cloned, and tissue distributions showed that the four CTLs were ubiquitously expressed in all the tested tissues, and mainly expressed in hepatopancreas and gills. The transcription of the four CTLs significantly increased in several immune-related tissues (hemocytes, hepatopancreas and gills) of P. trituberculatus challenged with Vibrio alginolyticus and displayed different profiles. Moreover, the four CTLs displayed distinct bacterial binding and antibacterial activities. The recombinant protein PtCTL-1 (rPtCTL-1) and rPtCTL-3 displayed bacterial binding and antibacterial activities against all tested bacteria. rPtCTL-2 only showed bacterial binding and antibacterial activities against V. alginolyticus. No obvious bacterial binding or antibacterial activities for PtCTL-4 was observed against the tested bacteria. This study enriches the transcriptomic information on P. trituberculatus and provides new insights into the innate immune system of crustaceans. Additionally, our study provided candidates of antibiotic agents for the prevention and treatment of bacteriosis.

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Identification of two novel C-type lectins involved in immune defense against white spot syndrome virus and Vibrio parahaemolyticus from Marsupenaeus japonicus

Cited by 14 publications

References 48 publications

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Full-Length Transcriptome Analysis Provides New Insights Into the Diversity of Immune-Related Genes in Portunus trituberculatus

Proteomic analysis of the hepatopancreas of Exopalaemon carinicauda in response to Metschnikowia bicuspidate infection

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