Identification of Two Long Non-Coding RNAs AC010082.1 and AC011443.1 as Biomarkers of Coronary Heart Disease Based on Logistic Stepwise Regression Prediction Model

Abstract

Coronary heart disease (CHD) is a global health concern with high morbidity and mortality rates. This study aimed to identify the possible long non-coding RNA (lncRNA) biomarkers of CHD. The lncRNA- and mRNA-related data of patients with CHD were downloaded from the Gene Expression Omnibus database (GSE113079). The limma package was used to identify differentially expressed lncRNAs and mRNAs (DElncRNAs and DEmRNAs, respectively). Then, miRcode, TargetScan, miRDB, and miRTarBase databases were used to form the competing endogenous RNA (ceRNA) network. Furthermore, SPSS Modeler 18.0 was used to construct a logistic stepwise regression prediction model for CHD diagnosis based on DElncRNAs. Of the microarray data, 70% was used as a training set and 30% as a test set. Moreover, a validation cohort including 30 patients with CHD and 30 healthy controls was used to verify the hub lncRNA expression through real-time reverse transcription-quantitative PCR (RT-qPCR). A total of 185 DElncRNAs (114 upregulated and 71 downregulated) and 382 DEmRNAs (162 upregulated and 220 downregulated) between CHD and healthy controls were identified from the microarray data. Furthermore, through bioinformatics prediction, a 38 lncRNA-21miRNA-40 mRNA ceRNA network was constructed. Next, by constructing a logistic stepwise regression prediction model for 38 DElncRNAs, we screened two hub lncRNAs AC010082.1 and AC011443.1 (p < 0.05). The sensitivity, specificity, and area under the curve were 98.41%, 100%, and 0.995, respectively, for the training set and 93.33%, 91.67%, and 0.983, respectively, for the test set. We further verified the significant upregulation of AC010082.1 (p < 0.01) and AC011443.1 (p < 0.05) in patients with CHD using RT-qPCR in the validation cohort. Our results suggest that lncRNA AC010082.1 and AC011443.1 are potential biomarkers of CHD. Their pathological mechanism in CHD requires further validation.

Upregulation of the Long Non-coding RNA LINC01480 Is Associated With Immune Infiltration in Coronary Artery Disease Based on an Immune-Related lncRNA-mRNA Co-expression Network

“…Xu et al found that eight epithelial-mesenchymal transition-related lncRNAs are potential biomarkers in CAD ( 42 ). Liu et al found that AC010082.1 and AC011443.1 could be biomarkers of CAD based on the ceRNA mechanism and logistic stepwise regression prediction model, and they validated that these lncRNAs were upregulated in CADs using RT-PCR ( 43 ). In this study, we also uncovered two new lncRNAs (AL359237.1 and LINC01480) by mining the GSE113079 dataset, which might predict the progression of atherosclerosis.…”

Efficacy and safety of the Chinese herbal medicine Bu-Shen-Huo-Xue granule for the treatment of coronary heart disease: study protocol for a multicenter, randomized, double-blinded, placebo-controlled clinical trial

“…A growing number of epidemiological studies have reported a link between chronic kidney disease (CKD) and CHD, perhaps providing another perspective on clinical treatment. Traditional Chinese Medicine (TCM) has been widely used in treating CHD for a long time and studies have confirmed the biological mechanism ( 3 , 4 ). With the change of social lifestyle, the TCM syndrome for unstable angina pectoris with coronary heart disease presents a combination form of “kidney deficiency and blood stasis.” A clinical epidemiological survey on the distribution of TCM syndromes showed that qi deficiency was the main symptom of angina pectoris in coronary heart disease, accounting for up to 78.8%, second only to blood stasis, of which heart and kidney qi deficiency was the mainstay ( 5 ).…”

“…A growing number of epidemiological studies have reported a link between chronic kidney disease (CKD) and CHD, perhaps providing another perspective on clinical treatment. Traditional Chinese Medicine (TCM) has been widely used in treating CHD for a long time and studies have confirmed the biological mechanism (3,4). With the change of social lifestyle, the TCM syndrome for unstable angina pectoris with coronary heart disease presents a combination form of "kidney deficiency and blood stasis."…”