Identification of Two Dominant Serotypes of Rhizobium trifolii in Root Nodules of Uninoculated Field‐Grown Subclover

Abstract

Analysis of the protein profile patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) of 30 isolates of Rhizobium trifolii obtained from root‐nodules of uninoculated field grown plants of subclover (Trifolium subterraneum L.) cv. ‘Mt. Barker’ showed that 12 and 9 isolates were represented by two different protein profile patterns (gel types). Serological analyses performed using antisera raised to one isolate of each gel type showed that although the two isolates, 1‐01 and 2‐01, shared a common antigenic determinant, they were distinguishable by their reactions in whole‐cell somatic agglutination and gel‐immune‐diffusion tests. Forty‐nine of the sixty isolates collected from nodules in the establishment year, and 56 of 90 isolates collected the following year were antigenically identical to either one or the other of the two parent isolates. Sixteen of the one hundred fifty isolates cross‐reacted strongly with both antisera in whole‐cell somatic tube agglutination and gel‐immune‐diffusion assays while only 23 of the 150 isolates were antigenically unrelated to either 1‐01 or 2‐01. All of the 22 isolates of serotype 1‐01 collected in the establishment year possessed the same gel type, wheres 19 of 27 isolates of serotype 2‐01 had the same gel type as the parent isolate, 2‐01. The majority (18 of 22) of isolates of serotype 1‐01 were superior symbiotic effectiveness while the majority (17 of 19) of isolates of serotype 2‐01 possessing the same gel type were of inferior effectiveness.

Autecology in Rhizospheres and Nodulating Behavior of Indigenous Rhizobium trifolii

“…Fluorescent-antibody preparation. Antisera were prepared against somatic antigens of R. trifolii strains 1-01 and 2-01, as previously described (9).…”

Influence of Location, Host Cultivar, and Inoculation on the Composition of Naturalized Populations of Rhizobium meliloti in Medicago sativa Nodules

“…Nevertheless, serological analysis has provided information on the impact of environmental (6, 7, 15) and biotic (5) factors on the serogroup distribution of indigenous Rhizobium japonicum inhabiting nodules. More recently, separation of cellular proteins by gel electrophoresis has shown considerable variety within nodule populations of indigenous R. japonicum (26) and among isolates of indigenous Rhizobium trifolii (8,10,11).…”

Dual Inoculation Response of Soybean with Rhizobium And Mycorrhiza

“…특히 시 험된 토양에는 Bradyrhizobium japonicum이 토양 g 당 1 × . 일반적으로 토착 근류 균이 서식하는 토양에서 접종균주의 뿌리혹형성 경합력은 20% 이내인 것으로 알려져 있으며 (Demezas and Bottomly, 1984), 접종 근류균의 뿌리혹 점유율을 50% 수준으로 올리 는 데는 토착균주의 서식밀도보다 1,000배가량 많은 근류균 의 접종이 필요한 것으로 알려져 있다 (Weaver and Frederick, 1974 (Table 3 참조) . 그리고 Table 2의 이상의 결과로 볼 때, 우리나라처럼 근류균과 균근균이 다양하게 분포하는 농경지에서 (Kang, 1998;Kang et al, 1999;Park et al, 1999b) …”