Identification of two differentially expressed forms of progranulin mRNA in the teleost fish Paralichthys olivaceus

Nam, Bo‐Hye; Park, Eun Mi; Kim, Young‐Ok; Kong, Hee Jeong; Kim, Woo Jin; Kang, Jung-Ha; Ji, Young-Ju; Lee, Sang‐Jun; Choi, Tae-Jin

doi:10.1016/j.fsi.2008.09.009

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…and plays an important role in fish development and innate immune response. Previously, two isoforms of pgrn mRNA were isolated from head kidney and spleen cDNA libraries of the olive flounder P. olivaceus , probably encoding proteins with distinct activity in the immune system of P. olivaceus (Nam et al ., 2009). Furthermore, pgrn and granulin (GRN) peptides may exert opposing immunoregulatory activity during wound healing (Zhu et al ., 2002).…”

Section: Discussionmentioning

confidence: 99%

Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae

Wang

Chen

et al. 2013

Journal of Fish Biology

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Two subtractive complementary DNA libraries were constructed from Nile tilapia Oreochromis niloticus vaccinated with formalin-killed Streptococcus iniae cells, and a further two constructed from O. niloticus infected with S. iniae. Of the 68 distinct expressed sequence tag (EST) contigs and singletons, 45 and 13 EST shared high similarities with genes of known and unknown functions, respectively. Ten EST contigs and singletons had no significant similarity to any sequences. Five putative immune-relevant genes, β2m, α-ha, mmp9, pgrn and cxcr4, were selected for quantitative reverse-transcription polymerase chain reaction in four strains of Oreochromis spp.: genetically improved farmed tilapia (O. niloticus), Oreochromis aureus, O. niloticus and O. niloticus×O. aureus, with different disease resistance following infection with S. iniae. pgrn was up-regulated more significantly in disease-resistant strains than in the susceptible. α-ha was markedly down-regulated, and no significant differences in the expression level of β2m were detected. A negative correlation was observed between the expression of mmp9 and that of cxcr4. The results provide insight into the molecular response of O. niloticus to S. iniae infection.

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Section: Discussionmentioning

confidence: 99%

Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae

Wang

Chen

et al. 2013

Journal of Fish Biology

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“…The agarose gel bands were scanned using computer-by-computer analysis, and the optical density was plotted as a histogram. The Bio-Rad Gel-Doc system was used to quantify the PCR data, which were normalized to the respective β-actin expression levels (Nam et al 2009). All assays were performed in triplicate as independent experiments.…”

Section: Animal Cells and Systemsmentioning

confidence: 99%

Molecular characterization and gene expression analysis of a metalloprotease from Pacific abaloneHaliotis discus hannai

Kim

Han²,

Moon

et al. 2014

Animal Cells and Systems

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We isolated a metalloprotease (MP) homologue from an abalone muscle cDNA library. A 3284-kb full-length cDNA encoding a predicted polypeptide of 667 amino acids was sequenced. The abalone MP Haliotis discus hannai (HdMP) exhibited a domain structure typical of the peptidase M4 family, a 21-amino acid N-terminal hydrophobic signal sequence followed by a long propeptide sequence of 347 amino acids and the mature protease domain comprising 299 amino acids. The mature region contains features characteristic of a zinc protease, including a zinc-binding motif (HEXXH) and an active site. The protein showed 32-38% amino acid sequence identity with other known MP sequences and with a hypothetical protein from owl limpet. The mRNA transcript is expressed in almost all tissues, with high expression in the mantle and adductor muscle of healthy abalones, and is expressed constitutively during the early developmental stages after fertilization. Lipopolysaccharide or poly I:C stimulation induced the expression of the HdMP transcript in the digestive track and gills of abalones. Collectively, these results suggest that HdMP could play multiple roles in defense, the immune response, growth, and regulation of reproduction.

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“…The granulin/epithelin precursor (GEP), also known as proepithelin, acrogranin, progranulin (PGRN) and PC cellderived growth factor (PCDGF), encodes a glycoprotein precursor which exhibits pleiotropic tissue growth factor activity with multiple functions, i.e., in biomineralization in mammals (Cao et al 2010;Feng et al 2010;Zhao et al 2013a), and is found in animals ranging from sponges to mammals (Bhandari et al 1992;Nara et al 2004;Hanington et al 2008;Srivastava et al 2008;Nam et al 2009;Smout et al 2009;Srivastava et al 2010;Zhao et al 2013b). Meanwhile, there is still unknown that whether GEP existed and involved in the biomineralization process for the pearl oyster, Pinctada fucata, which is an important marine bivalve species cultured for pearl production and a hot research topic in biomineralization.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, GEP was shown to have a novel critical role in biomineralization in mammals (Cao et al 2010;Feng et al 2010;Zhao et al 2013a). In non-mammals, GEP proteins are reported to have a growth modulatory function (Belcourt et al 1993;Hanington 2006;Hanington et al 2008), thrombin inhibitory activity (Jin Hong and Won Kang 1999), antitumor effect (Zhao et al 2013b), and immune function (Nam et al 2009). Despite these advances, few studies have focused on the mechanism of GEP transcript variants' generation and the function of each GEP variant, except in teleost fish.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization and molecular mechanism exploration of three granulin epithelin precursor splice variants in biomineralization of the pearl oyster Pinctada fucata

Zhao

Huang

et al. 2015

Mol Genet Genomics

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The granulin/epithelin precursor (GEP) encodes a glycoprotein precursor which exhibits pleiotropic tissue growth factor activity with multiple functions. Here, GEP was isolated and its role in the shell biomineralization process of the pearl oyster Pinctada fucata was investigated. Three forms of GEP mRNA were isolated from the pearl oyster (designated PfGEP-1, PfGEP-2 and PfGEP-3). Genomic DNA flanking the splicing region of the PfGEP variants was sequenced and it was found that PfGEP-2 splices out Exon 4, whereas PfGEP-3 splices out Exon 3 compared to PfGEP-1. PfGEP-1 (1505 amino acids) consists of 18 granulin domains, whereas PfGEP-2 (1459 amino acids) and PfGEP-3 (1471 amino acids) consist of 17.5 granulin domains, respectively. Analyses of PfGEP-1 and PfGEP-3 mRNA showed differential patterns in the tissues and developmental stages. Western blotting results showed that the three splice variants can translate to proteins in HEK293T cells. A knockdown experiment using PfGEP dsRNA showed decreased PfGEP-1/PfGEP-3 and PfMSX mRNA, and irregular crystallization of the nacreous layer using scanning electron microscopy. In luciferase assays, co-transfection of PfGEP-1 could activate as well as repress luciferase expression of the reporter plasmid driven by the PfMSX promoter, whereas PfGEP-3 stimulated the expression, elucidating the molecular mechanisms involved in the correlation between PfGEP and PfMSX. These results suggested that GEP variants might function differently during the biomineralization process, which provides new knowledge on the mechanism regulating nacre formation.

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Identification of two differentially expressed forms of progranulin mRNA in the teleost fish Paralichthys olivaceus

Cited by 5 publications

References 25 publications

Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae

Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae

Molecular characterization and gene expression analysis of a metalloprotease from Pacific abaloneHaliotis discus hannai

Functional characterization and molecular mechanism exploration of three granulin epithelin precursor splice variants in biomineralization of the pearl oyster Pinctada fucata

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