Identification of tropomyosin, paramyosin and apolipophorin/vitellogenin as three major allergens of the sheep scab mite, Psoroptes ovis

Abstract

Analysis by one-dimensional (1-D) SDS-PAGE/Western blotting of whole mite extract (larval and adult stages) with sheep sera taken 0-84 days after infection with the sheep scab mite, Psoroptes ovis revealed a progressive IgE antibody response, with a dominant high molecular weight allergen (MW 180 kDa) detected early during infection. Further analysis by two-dimensional (2-D) SDS-PAGE/Western blotting with post-infection sera, revealed a more complex picture with numerous (> 20) IgE reactive spots. Trypsin digest and Maldi-ToF analyses of these spots identified two house dust mite allergen homologues, namely tropomyosin (Der p 10) and paramyosin (Der p 11), and analysis of a third spot indicated an apolipoprotein-like IgE reactive protein (Der p 14). Further 1-D and 2-D SDS/Western blotting, with specific antibodies to the house dust mite allergens Der p 10, 11, and to the IgE reactive peptide of the high molecular weight house dust mite allergen, Der p 14 (vitellogenin/apolipophorin), provided firm evidence for the presence of these three allergens in extracts of the Psoroptes mite. These studies show for the first time that homologues of the house dust mite 10, 11 and 14 group allergens are represented as immunodominant allergens of the sheep scab mite, and may represent important vaccine candidates.

Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus

“…For example, the important P. ovis mite allergens Pso o 2, Pso o 8, Pso o 10, Pso o 11, Pso o 14 and many more antigens, each have ontological equivalents in D. pteronyssinus termed Der p 2, Der p 8, Der p 10, Der p 11 and Der p 14, respectively [39,40]. Given the similarities observed, the development of RNAi in D. pteronyssinus could be considered as a method of developing protocols for RNAi in other astigmatid mites of medical and veterinary importance, including P. ovis and Sarcoptes scabiei that cannot be cultured in vitro, are difficult to reliably harvest in sufficient numbers, and for which there is currently a paucity of genomic information [26,41].…”

Cross-reactivity between storage and dust mites and between mites and shrimp

“…The epitopes differ in only 1-6 amino acid residues. A homolog of Der p 10 allergen is an immunodominant allergen of the sheep mite Psoroptes ovis (Huntley et al 2004). Also, tropomyosin is a ubiquitous protein in the tissues of the parasitic red poultry mite Dermanyssus gallinae and has an 85% identity to Der p 10 (Nisbet et al 2006).…”

Understanding the biology and control of the poultry red miteDermanyssus gallinae: a review

“…Due to the increase in expression of Cathepsin L and vitellogenin in fed mites, it is plausible that Cathepsin L-like proteases could play a part in PRM vitellogenesis. Huntley et al (2004) describe a vitellogenin homologue in the sheep scabies mite P. ovis to be highly immunogenic to the host. It is hypothesized P. ovis may induce an allergic response to aid feeding and thus pre-vaccination of allergens such as vitellogenin may inhibit the induction of pro-inflammatory IgE antibodies and influence mite feeding.…”