Identification of tRNA-derived Fragments and their Potential Roles in Diabetic Cataract Rats

Han, Xiao; Cai, Lei; Lü, Yi; Li, Dan; Yang, Jin

doi:10.2217/epi-2020-0193

Cited by 15 publications

(10 citation statements)

References 32 publications

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“…Nowadays, increasing studies have identified and emphasized the important role of tsRNAs in various diseases. 7,9,16,17 Therefore, we herein aimed to explore the expression pattern of tsRNAs, as well as the potential roles of candidate tsRNAs in the development of LN. Of note, our sequencing and qRT-PCR data showed that the expression levels and subtypes of tsRNAs were significantly altered in renal tissues of LN patients compared with the control.…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis

Liang

Zhang

Qiu

et al. 2021

Lupus

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Objective Lupus nephritis (LN) is a major end-organ complication of systemic lupus erythematosus (SLE), and the molecular mechanism of LN is not completely clear. Accumulating pieces of evidence indicate the potential vital role of tRNA-derived small RNAs (tsRNAs) in human diseases. Current study aimed to investigate the potential roles of tsRNAs in LN. Methods We herein employed high‐throughput sequencing to screen the expression profiles of tsRNAs in renal tissues of the LN and control groups. To validate the sequencing data, we performed quantitative real-time PCR (qRT-PCR) analysis. Correlational analysis of verified tsRNAs expression and clinical indicators was conducted using linear regression. The potential target genes were also predicted. The biological functions of tsRNAs were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results Our findings revealed that the expression profiles of tsRNAs were significantly altered in the kidney tissues from LN patients compared with control. Overall, 160 tsRNAs were significantly dysregulated in the LN group, of which 79 were upregulated, whereas 81 were downregulated. Subsequent qRT-PCR results confirmed the different expression of candidate tsRNAs. Correlation analysis results found that expression of verified tsRNAs were correlated to clinical indicators. The target prediction results revealed that verified tsRNAs might act on 712 target genes. Further bioinformatics analysis uncovered tsRNAs might participate in the pathogenesis of LN through several associated pathways, including cell adhesion molecules, MAPK signaling pathway, PI3K-Akt signaling pathway and B cell receptor signaling pathway. Conclusion This study provides a novel insight for studying the mechanism of LN.

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Section: Discussionmentioning

confidence: 99%

Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis

Liang

Zhang

Qiu

et al. 2021

Lupus

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“…This research produced the first expression profile of tRFs/tiRs in DC rats and found that four types of tRFs/tiRs (tiRNA-Gly-GCC-002, tRF-Tyr-GTA-029, tRF-Thr-TGT-039 and tRF-Gln-CTG-043) are involved in the pathogenesis of DC. The identification of these tRFs/tiRs may provide a new perspective for further studies investigating the exact mechanism of DC [ 103 ].…”

Section: Trfs In Age-related Diseasesmentioning

confidence: 99%

tRNA-derived fragments as New Hallmarks of Aging and Age-related Diseases

Yuán¹,

Li²,

He³

et al. 2021

Aging and disease

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“…They can be utilized as therapeutic targets and diagnostic biomarkers in tumor treatment ( Lee et al, 2009 ; Goodarzi et al, 2015 ; Olvedy et al, 2016 ; Huang et al, 2017 ; Zhu et al, 2020 ). Although tRFs in ophthalmic diseases have been widely researched, including diabetic cataracts and uveal melanoma ( Han et al, 2020 ; Londin et al, 2020 ), tRF expression and its potential roles in TAO have not yet been analyzed. We speculate that tRFs may be upregulated in TAO patients and are likely to have a regulatory effect on TAO pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Identification of tRNA-Related Fragments and Their Potential Regulatory Effects in Thyroid-Associated Ophthalmopathy

et al. 2022

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Recently, the potential role of tRNA-related fragments (tRFs) in ophthalmic diseases has been extensively researched. However, systematic studies on the potential regulatory effects of tRFs in thyroid-associated ophthalmopathy (TAO) are lacking. We used high-throughput sequencing techniques to measure expression levels of mRNAs and tRFs in patients with TAO, and the results were verified by real-time quantitative reverse transcription polymerase chain reaction (q-PCR). Next, the potential biological regulatory effect of differentially expressed tRFs was analyzed, and potential downstream target RNAs of differentially expressed tRFs were predicted to explore the potential role of tRFs as therapeutic targets and biomarkers of TAO. A total of 50 tRFs and 361 mRNAs were dysregulated in the TAO group, and tRF5-GluCTC, PMAIP1, HSD17B2 and ATF3 were verified to be significantly differentially expressed in TAO. Our research reveals that several associated pathways likely play a role in the pathogenesis of TAO. By targeting ATF3, HSD17B2 and PMAIP1, tRF5-GluCTC may play a potential role in regulating the orbital fibroblast adipogenic response and fibrotic hyperplasia in patients with TAO.

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Identification of tRNA-derived Fragments and their Potential Roles in Diabetic Cataract Rats

Cited by 15 publications

References 32 publications

Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis

Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis

tRNA-derived fragments as New Hallmarks of Aging and Age-related Diseases

Identification of tRNA-Related Fragments and Their Potential Regulatory Effects in Thyroid-Associated Ophthalmopathy

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