Identification of triterpenoid saponins in flowers of four Camellia Sinensis cultivars from Zhejiang province: Differences between cultivars, developmental stages, and tissues

Xian, Shi; Shi, Linzuo; Pan, Haibo; Li, Bo; Wu, Yuanyuan; Tu, Youying

doi:10.1016/j.indcrop.2016.10.008

Cited by 21 publications

(21 citation statements)

References 21 publications

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“…UPLC (Waters Corporation, USA) was employed to analyse the content of triterpenoid saponins in tea flowers, equipped with a Welch Ultimate LP-C18 column (4.6 mm 9 250 mm i.d., 3 lm), according to the method of Shen et al (2017). A total of 10 lL of sample solution was analysed using gradient elution with solvent A (formic acid/water (0.1:99.9, v/v)) and solvent B (acetonitrile), at a flow rate of 0.6 mL min À1 at 25°C.…”

Section: Analysis Of Triterpenoid Saponins By Uplcmentioning

confidence: 99%

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Hot air drying of tea flowers: effect of experimental temperatures on drying kinetics, bioactive compounds and quality attributes

Shi

et al. 2018

Int J of Food Sci Tech

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Summary The drying process is important for the quality and value of tea flower products. In the present work, the effects of different hot air drying (HAD) temperatures from 60 to 120 °C on drying kinetics, bioactive compounds (catechins, flavonol glycosides and triterpenoid saponins) and quality attributes (volatile compounds, colours and antioxidant properties) of tea flowers were systematically evaluated. The results showed that higher drying temperature resulted in a shorter drying time but with greater loss of bioactive compounds and quality attributes. Flowers subjected to HAD at 60 °C showed the highest bioactive contents and quality attributes of all HAD samples and the quality of HAD 60 °C samples were close to that of freeze‐dried samples. Taking account of the production efficiency and energy consumption, the tea flowers dried at 60 °C for 180 min was preferred. These findings provide a guide for the processing of tea flowers with the aim of improving the overall quality of the product.

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Section: Analysis Of Triterpenoid Saponins By Uplcmentioning

confidence: 99%

“…Floratheasaponins and Chakasaponins, two major triterpenoid saponins, are present at high concentrations in Chinese tea flowers. Here, triterpenoid saponins in tea flower samples produced at different temperatures were measured by the UPLC method (Shen et al, 2017). Seven main triterpenoid saponins were detected and listed (Fig.…”

Section: Triterpenoid Saponinsmentioning

confidence: 99%

Hot air drying of tea flowers: effect of experimental temperatures on drying kinetics, bioactive compounds and quality attributes

Shi

et al. 2018

Int J of Food Sci Tech

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“…The aqueous extract was separated by AB-8 macroporous adsorption resin, followed by preparative HPLC to obtain BTFS. BTFS was then identified by UPLC-Q-TOF/MS/MS system with ultra-high performance chromatography (Agilent, Santa Clara, CA, USA) coupled with electro-spray ionization quadrupole time-of-flight mass spectrometry (AB SCIEX, Framingham, MA, USA) according to the methods we described previously, with certain modifications [20]. BTFS was dissolved in dimethyl sulfoxide (DMSO) to make a 20 mg/mL stock solution and stored at −20 • C before use.…”

Section: Extraction and Identification Of Baiye No1 Tea Flower Saponmentioning

confidence: 99%

Standardized Saponin Extract from Baiye No.1 Tea (Camellia sinensis) Flowers Induced S Phase Cell Cycle Arrest and Apoptosis via AKT-MDM2-p53 Signaling Pathway in Ovarian Cancer Cells

Chen

Ren

et al. 2020

Molecules

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Ovarian cancer is considered to be one of the most serious malignant tumors in women. Natural compounds have been considered as important sources in the search for new anti-cancer agents. Saponins are characteristic components of tea (Camellia sinensis) flower and have various biological activities, including anti-tumor effects. In this study, a high purity standardized saponin extract, namely Baiye No.1 tea flower saponin (BTFS), which contained Floratheasaponin A and Floratheasaponin D, were isolated from tea (Camellia sinensis cv. Baiye 1) flowers by macroporous resin and preparative liquid chromatography. Then, the component and purity were detected by UPLC-Q-TOF/MS/MS. This high purity BTFS inhibited the proliferation of A2780/CP70 cancer cells dose-dependently, which is evidenced by the inhibition of cell viability, reduction of colony formation ability, and suppression of PCNA protein expression. Further research found BTFS induced S phase cell cycle arrest by up-regulating p21 proteins expression and down-regulating Cyclin A2, CDK2, and Cdc25A protein expression. Furthermore, BTFS caused DNA damage and activated the ATM-Chk2 signaling pathway to block cell cycle progression. Moreover, BTFS trigged both extrinsic and intrinsic apoptosis—BTFS up-regulated the expression of death receptor pathway-related proteins DR5, Fas, and FADD and increased the ratio of pro-apoptotic/anti-apoptotic proteins of the Bcl-2 family. BTFS-induced apoptosis seems to be related to the AKT-MDM2-p53 signaling pathway. In summary, our results demonstrate that BTFS has the potential to be used as a nutraceutical for the prevention and treatment of ovarian cancer.

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“…The rate of the mobile phase was 1.5 mL/min. After separation, PTFSs were collected and measured by UPLC coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS/MS), as previously explained [23]. PTFSs were dissolved in DMSO to prepare a solution with the final concentration of 20 mg/mL, and was kept at −20 • C DMSO was bought from Sigma-Aldrich (Sigma, St. Louis, MO, USA).…”

Section: Preparation Of Purified Tea Flower Saponins (Ptfss)mentioning

confidence: 99%

“…Triterpenoid saponins are characteristic ingredients and have a higher content in tea flowers compared with that in tea leaves. We previously identified 21 triterpenoid saponins in extracts from tea flowers in selected cultivars [23]. In recent years, the bio-functional effects of tea flower saponins such as antihyperlipidemic, antihyperglycemic, anti-obesity, anti-cancer, and gastroprotective effects have been reported [24][25][26], among which Chakasaponin I has been reported to have antihyperlipidemic and antihyperglycemic [27], anti-cancer [26], and anti-obesity [28] effects.…”

Section: Introductionmentioning

confidence: 99%

Purified Tea (Camellia sinensis (L.) Kuntze) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells

Ren

Chen

et al. 2020

IJMS

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Ovarian cancer is currently ranked at fifth in cancer deaths among women. Patients who have undergone cisplatin-based chemotherapy can experience adverse effects or become resistant to treatment, which is a major impediment for ovarian cancer treatment. Natural products from plants have drawn great attention in the fight against cancer recently. In this trial, purified tea (Camellia sinensis (L.) Kuntze) flower saponins (PTFSs), whose main components are Chakasaponin I and Chakasaponin IV, inhibited the growth and proliferation of ovarian cancer cell lines A2780/CP70 and OVCAR-3. Flow cytometry, caspase activity and Western blotting analysis suggested that such inhibitory effects of PTFSs on ovarian cancer cells were attributed to the induction of cell apoptosis through the intrinsic pathway rather than extrinsic pathway. The p53 protein was then confirmed to play an important role in PTFS-induced intrinsic apoptosis, and the levels of its downstream proteins such as caspase families, Bcl-2 families, Apaf-1 and PARP were regulated by PTFS treatment. In addition, the upregulation of p53 expression by PTFSs were at least partly induced by DNA damage through the ATM/Chk2 pathway. The results help us to understand the mechanisms underlying the effects of PTFSs on preventing and treating platinum-resistant ovarian cancer.

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Identification of triterpenoid saponins in flowers of four Camellia Sinensis cultivars from Zhejiang province: Differences between cultivars, developmental stages, and tissues

Cited by 21 publications

References 21 publications

Hot air drying of tea flowers: effect of experimental temperatures on drying kinetics, bioactive compounds and quality attributes

Hot air drying of tea flowers: effect of experimental temperatures on drying kinetics, bioactive compounds and quality attributes

Standardized Saponin Extract from Baiye No.1 Tea (Camellia sinensis) Flowers Induced S Phase Cell Cycle Arrest and Apoptosis via AKT-MDM2-p53 Signaling Pathway in Ovarian Cancer Cells

Purified Tea (Camellia sinensis (L.) Kuntze) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells

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