Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes

Li, Jiuxuan; Wāng, Ying; Jin, Huifang; Li, Wujiao; Yan, Chaochao; Yan, Pengfei; Zhang, Xiuyue; He, Shunping; Song, Zhaobin

doi:10.1016/j.gene.2016.11.045

Cited by 15 publications

(13 citation statements)

References 43 publications

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“…In 1992, 33 Triplophysa species were identified. However, over time, a large number of new species have been described, and a total of 140 valid species have been identified [27,40]. Although some synonymous species may occur [27,55], these studies showed that a large amount of unknown biodiversity exists in the genus Triplophysa, and many species have not been recognized or described.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

Wang

Zhang²,

Yang³

et al. 2020

BMC Evol Biol

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Background The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results After careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions. Conclusions The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

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Section: Introductionmentioning

confidence: 99%

DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

Wang

Zhang²,

Yang³

et al. 2020

BMC Evol Biol

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“…6–15 transverse stripes on side and back and brindle coloured). Furthermore, the genetic distance between the new species and T. dorsalis , T. elegans and T. strauchii (7.86%, 6.14% and 8.42%, respectively) based on coI markers is concordant with species‐level divergence in other fish taxa (Fadli et al ., 2020; Li et al ., 2017; Ward et al ., 2005; Figure 6).…”

Section: Discussionmentioning

confidence: 99%

Triplophysa ferganaensis, a new loach species from Fergana Valley in Central Asia (Teleostei: Nemacheilidae)

Sheraliev

Peng

2021

Journal of Fish Biology

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Triplophysa ferganaensis sp. nov. is described from the Shakhimardan stream, a small tributary of the Syr Darya, which does not reach the river in Fergana Valley. It can be distinguished from other valid Triplophysa loaches based on the following combination of characters: body smooth and scaleless, lateral line complete, posterior chamber of air bladder degenerated, inner gill rakers 10–11 on the first‐gill arch, outer gill rakers absent, vertebrae 4 + 35–36, 8 + 8 branched caudal‐fin rays, caudal peduncle depth 2.1–2.7 times its length, two supratemporal pores, dorsal‐fin origin closer to the caudal‐fin base than to the snout tip, caudal fin emarginated and pelvic‐fin tip reaching the anus. The new species can also be distinguished from its congeners based on the molecular analyses of mitochondrial cytochrome oxidase subunit I (coI) gene sequences. The phylogenetic position of this new species indicates that it is a sister taxon of Triplophysa tenuis.

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“…In 1992, there were 33 Triplophysa species identi ed. However, over time, a large number of new species have been described; so far there are a total of 140 valid species (He et al, 2008;Li et al,2017;). Although there may be some synonyms species (He et al, 2008;Proko ev et al, 2007), these studies show that a large amount of unknown biodiversity exists in the Triplophysa, and many species have not been recognized or described.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that species identi cation results based on DNA barcoding, which is one of the effective means to identify morphologically indistinguishable cryptic species, have a high degree of matching with the current classi cation system (Barrett et al, 2005;Dincǎ et al, 2011;Dhar et al, 2017). Triplophysa species can also be identi ed by DNA barcoding (Li et al, 2017). To date, there are no studies on the identi cation or evaluation of cryptic biodiversity within the genus Triplophysa using DNA barcoding in northeastern QTP.…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding Reveals Cryptic Diversity in the Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

wang

Zhang²,

Yang³

et al. 2020

Preprint

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Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the taxonomic characteristics of the plateau loach by means of morphology, DNA barcoding and multiple species demarcation methods to accurately describe species and allocate taxonomic units to unknown specimens. Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, had a disputed taxonomic status, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

show abstract

Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes

Cited by 15 publications

References 43 publications

DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

Triplophysa ferganaensis, a new loach species from Fergana Valley in Central Asia (Teleostei: Nemacheilidae)

DNA Barcoding Reveals Cryptic Diversity in the Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

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