Identification of trigger factors selecting for polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge sequencing batch reactors

Weissbrodt, David G.; Schneiter, Guillaume Stéphane; Fürbringer, Jean-Marie; Holliger, Christof

doi:10.1016/j.watres.2013.08.043

Cited by 67 publications

(67 citation statements)

References 38 publications

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“…The results of the present study, concerning the higher PAO/GAO level in GSBR fed with lower COD/P ratios, are inconsistent with the findings of Weissbrodt et al (2013). They concluded that higher ratios of Accumulibacter to Competibacter can be obtained with higher COD/P, whereas lower COD concentrations favour mainly Competibacter and Tetrasphaera.…”

Section: Resultscontrasting

confidence: 99%

“…It may be concluded that low COD/P ratio promotes growth of PAOs, which belong mainly to Accumulibacter and, to less extent, to Tetrasphaera. Weissbrodt et al (2013) proved that Accumulibacter selection in AGS can be favoured in the presence of propionate when compared to acetate only (25-40 % and 6 % of EUBmix, respectively). A significant percentage (up to 16 %) of Tetrasphaera was also detected in a laboratoryscale GSBR fed with acetate, but abundance of this putative PAOs progressively decreased to less than 5 % when propionate was used alone or in mixture with acetate (Weissbrodt et al 2013).…”

Section: Resultsmentioning

confidence: 96%

“…Weissbrodt et al (2013) proved that Accumulibacter selection in AGS can be favoured in the presence of propionate when compared to acetate only (25-40 % and 6 % of EUBmix, respectively). A significant percentage (up to 16 %) of Tetrasphaera was also detected in a laboratoryscale GSBR fed with acetate, but abundance of this putative PAOs progressively decreased to less than 5 % when propionate was used alone or in mixture with acetate (Weissbrodt et al 2013). Ahn et al (2009) detected Tetrasphaera-related PAOs with Actino-658 probe after starting GSBR only, which was inoculated with activated sludge from full-scale WWTP.…”

Section: Resultsmentioning

confidence: 96%

“…Therefore, it can be successfully used not only for nitrogen and phosphorus removal Wei et al 2014), but also for industrial wastewater treatment (Bumbac et al 2015) and toxic organic compound degradation (Zhu et al 2014). Community structure in aerobic granules is one of the most interesting topics for researchers (de Kreuk and van Loosdrecht 2004;de Kreuk et al 2007;Lee et al 2010;Weissbrodt et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…COD/P ratio affects the structure of bacterial communities involved in EBPR; however, majority of studies on PAO-GAO selection have been carried out on flocculent activated sludge as reviewed by Oehmen et al (2007). Very little knowledge is available on the PAO-GAO competition in AGS (de Kreuk and van Loosdrecht 2004;Lemaire et al 2008;Ahn et al 2009;Winkler et al 2011;Weissbrodt et al 2013). Lots of surveys focused on factors that influence granule formation and stability (Lee et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

Muszyński

Miłobędzka

2015

Int. J. Environ. Sci. Technol.

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A laboratory-scale granular sequencing batch reactor, fed with acetate, was operated at two different ratios of chemical oxygen demand to phosphorus-15:1 and 100:1. Smaller aerobic granules, but with better settleability, were obtained at the lower ratio. High ratio of phosphorus release to uptake of dissolved organic carbon (0.42 mol/mol) coincided with high percentage of polyphosphate-accumulating organisms (up to 70 % of all bacteria) and implied high metabolic activity of these bacteria. Polyphosphate-accumulating organisms belonged mainly to Accumulibacter and Tetrasphaera (46 and 23 %, respectively). Despite significant abundance, Tetrasphaera-related microorganisms were not detected by oligoprobes Actino-221 and Actino-658, but by broader oligoprobes Tet2-892 and Tet3-654. Low abundance (1 %) of Halomonas phosphatis indicated a minor role of these bacteria in the laboratory-scale reactor fed with synthetic wastewater. When the ratio of chemical oxygen demand to phosphorus was increased to 100:1, deterioration of settling properties was observed, caused by growth of filamentous organisms from Thiothrix/021N group. The higher ratio favoured Competibacter and was selected against all groups of polyphosphate-accumulating organisms. However, a significant percentage (10 %) of polyphosphate-accumulating organisms in the granular sludge with concomitant low ratio of phosphorus release to the uptake of dissolved organic carbon (0.01 mol/mol) suggested shift in the overall population metabolism. Under phosphorus limitation in wastewater, polyphosphate-accumulating organisms no longer synthesized poly-P and behaved as glycogen-accumulating organisms.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

Muszyński

Miłobędzka

2015

Int. J. Environ. Sci. Technol.

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Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Weissbrodt

Maillard

Brovelli

et al. 2014

Biotech & Bioengineering

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Enhanced biological phosphorus removal (EBPR) from wastewater relies on the preferential selection of active polyphosphate-accumulating organisms (PAO) in the underlying bacterial community continuum. Efficient management of the bacterial resource requires understanding of population dynamics as well as availability of bioanalytical methods for rapid and regular assessment of relative abundances of active PAOs and their glycogen-accumulating competitors (GAO). A systems approach was adopted here toward the investigation of multilevel correlations from the EBPR bioprocess to the bacterial community, metabolic, and enzymatic levels. Two anaerobic-aerobic sequencing-batch reactors were operated to enrich activated sludge in PAOs and GAOs affiliating with "Candidati Accumulibacter and Competibacter phosphates", respectively. Bacterial selection was optimized by dynamic control of the organic loading rate and the anaerobic contact time. The distinct core bacteriomes mainly comprised populations related to the classes Betaproteobacteria, Cytophagia, and Chloroflexi in the PAO enrichment and of Gammaproteobacteria, Alphaproteobacteria, Acidobacteria, and Sphingobacteria in the GAO enrichment. An anaerobic metabolic batch test based on electrical conductivity evolution and a polyphosphatase enzymatic assay were developed for rapid and low-cost assessment of the active PAO fraction and dephosphatation potential of activated sludge. Linear correlations were obtained between the PAO fraction, biomass specific rate of conductivity increase under anaerobic conditions, and polyphosphate-hydrolyzing activity of PAO/GAO mixtures. The correlations between PAO/GAO ratios, metabolic activities, and conductivity profiles were confirmed by simulations with a mathematical model developed in the aqueous geochemistry software PHREEQC.

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Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors

Weissbrodt

Holliger

Morgenroth

2017

Biotech & Bioengineering

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New-generation bioprocesses using granular sludge aim for a high-rate removal of nutrients from wastewater with low footprint. Achieving enhanced biological phosphorus removal (EBPR) relies on the design of sludge beds and wastewater feeding conditions to optimally load the biomass and to select for polyphosphate- (PAOs) over glycogen-accumulating organisms (GAOs) and over other heterotrophs. A hydraulic-metabolic mathematical model was developed to elucidate the impact of hydraulic transport patterns and environmental conditions on the PAO/GAO competition during up-flow feeding through an EBPR granular sludge bed. Tracer experiments highlighted plug-flow regimes with dispersion under both rapid (9 m h , Re = 1.6, Pe = 7.2, Pe = 4.6) and slow (0.9 m h , Re = 0.2, Pe = 21.3, Pe = 3.4) feeding. Non-turbulent regimes (Re << 10 ) promote a safe implementation of simultaneous fill/draw. Feeding time, pH, and temperature significantly impacted bacterial competition for carbon uptake under anaerobic slow feeding. Feeding duration should be designed to avoid full depletion of intracellular storage polymers within static granules. PAOs bear twice longer feeding than GAOs by using both polyphosphate and glycogen hydrolysis to sustain anaerobic C-uptake. Alkaline conditions (pH 7.25-8.0) by, e.g., dosing lime in the feed select for PAOs independently of temperature (10-30°C). A twice higher bed is required for full anaerobic conversions at 10 rather than 20°C. Biosystem responses for anaerobic C-uptake can be anticipated using the model toward designing robust anaerobic selectors to manage the microbial resource in EBPR granular sludge. Biotechnol. Bioeng. 2017;114: 1688-1702. © 2017 Wiley Periodicals, Inc.

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Identification of trigger factors selecting for polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge sequencing batch reactors

Cited by 67 publications

References 38 publications

The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

The effects of carbon/phosphorus ratio on polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge

Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors

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