Identification of Trichoderma spp. by DNA Barcode and Screening for Cellulolytic Activity

Abstract

J o u rn al of M ic ro b ia l & Bioc h e m ic a l Te chno lo g y

Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers

“…Species of the Trichoderma genus are typically found as soil-borne or wood-decaying fungi. Some species are economically important because they produce industrial enzymes (cellulase and hemicellulase) and antibiotics or act as biocontrol agents (Hassan 2014 andFahmi et al 2016). Therefore, correct identification of Trichoderma at the species level is important.…”

“…Many other researchers have used ITS sequences to identify Trichoderma spp. (Rifai 1969 andFahmi et al 2016). Multiple alignment of the ITS sequences of all seven Trichoderma species identified nucleotide sequence differences for differentiating these species.…”

“…Numerous molecular techniques have been used for Trichoderma identification to investigate the genetic diversity within the genus. These include restriction fragment length polymorphism (Dodd et al 2004), random amplified polymorphic DNA (RAPD) (Hermosa et al 2001;Parmar et al 2015a), amplified fragment length polymorphism (Devi et al 2013), inter-simple sequence repeat (ISSR) , microsatellite markers (Naef et al 2006), repetitive element sequence-based polymerase chain reaction (PCR) , and sequence analyses (Druzhinina et al 2005 andFahmi et al 2016).…”

Influence of Protoplast Fusion in Trichoderma Spp. on Controlling Some Soil Borne Diseases

“…This could be due to the production of diffusible components, such as lytic enzymes or water-soluble metabolites. These components, such as chitinase, protease and glucanase, were always secreted by Trichoderma at low concentration [5,25]. Therefore, they can act against the pathogenic fungi before mycelial contact, thus increasing the antagonism of Trichoderma.…”

Internal transcribed spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita

“…to be applied in the field of biological control is an important issue. It was reported that identification of Trichoderma based on morphological characters can give misleading results (Fahmi et al, 2016). Recently, molecular identification based on internal transcribed spacers (ITS) amplification and sequencing is common and highly trusted (Savitha and Sriam, 2015;Fahmi et al, 2016;Oskiera et al, 2015;Jiang et al, 2016).…”