Identification of Transmissible Viral Proventriculitis (TVP) in broiler chickens in Poland

Smiałek, M; Gesek, M.; Śmiałek, A; Koncicki, A.

doi:10.1515/pjvs-2017-0050

Cited by 6 publications

(11 citation statements)

References 4 publications

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“…Proventriculi from the rst Polish case of TVP [9] were used for the experimental infection. After homogenization, proventriculus samples were processed through three freeze-thaw cycles.…”

Section: Proventriculi Homogenate and Infectionmentioning

confidence: 99%

“…The rst reports on TVP date back to 1978 and come from the Netherlands [2], when Kouwenhoven et al reported a case of proventriculitis in commercial chicken broilers and proved that TVP was induced by an infectious factor. Since then, TVP cases have been identi ed and reported in the USA, Australia, China, South Korea, Spain, France, the UK, and Poland among other countries [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Given the recent cases of TVP recorded in Poland [9], a laboratory-conditions study was undertaken that attempted to reproduce the clinical course of TVP in broiler chickens by inoculating them with a homogenate of proventriculi from a con rmed TVP eld case. The research was also aimed at identifying the etiological factor in domestic cases of TVP.…”

Section: Introductionmentioning

confidence: 99%

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Chicken proventricular necrosis virus related transmissible viral proventriculitis in broiler chickens in Poland

Smiałek

Gesek

Dziwulska³

et al. 2020

Preprint

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Abstract Background. Transmissible viral proventriculitis (TVP) is an infectious disease reported in all production types of chickens. TVP is manifested in decreased body weight gains, wide weight diversity of birds in the flock and poor feed conversion. Histopathological examination seems to be the most reliable method for confirming the disease. Although TVP etiology has not been explicitly defined, a novel virus identified as a member of the Birnaviridae family, named chicken proventricular necrosis virus (CPNV) has been isolated from clinical cases of TVP and it is now considered as a potential factor of a disease. The study was undertaken in order to reproduce the disease under laboratory conditions and to evaluate the etiology of first described Polish case of TVP. Results. Anatomopathological and histopathological evaluation reveled that we've succeeded to reproduce TVP in broiler chickens. Within 14 days after infection birds gained approximately 30,38% less body weight in comparison to Control group. In TVP infected group a seroconversion against FAdV and IBDV was recorded 14 days post infection (dpi). 14 dpi CPNV was detected in proventriculi, while FAdV in spleens and livers of infected birds. Conclusions. We have demonstrated that CPNV was involved in the development of the disease. We did not record the presence of IBDV in the TVP or control birds, despite our recording a strong seroconversion against IBDV in the birds from the TVP group. CPNV belongs to the same family as IBDV, which allows us to assume serological cross-reactivity between them. This possibility of CPNV infections affecting IBDV antibody levels detected by commonly available ELISAs should be taken into account under poultry field conditions and diagnosis. The role of FAdV in the development of TVP needs further evaluation.

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“…Proventriculi from the rst Polish case of TVP [9] were used for the experimental infection. After homogenization, proventriculus samples were processed through three freeze-thaw cycles.…”

Section: Proventriculi Homogenate and Infectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chicken proventricular necrosis virus related transmissible viral proventriculitis in broiler chickens in Poland

Smiałek

Gesek

Dziwulska³

et al. 2020

Preprint

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“…The clinical signs of the disease are nonspecific and characterized by impaired growth, poor feed conversion, 2,19 nonuniformity of the flock, 2,16,19 and impaired digestion. 1,5,14 Mortality in flocks with TVP is reported to be 3.03–4.49%.…”

mentioning

confidence: 99%

“…These results are similar to other studies, 1,6,7,11,16 in which similar lesions have been reported as TVP cases in different countries. 1,10,14,19…”

mentioning

confidence: 99%

Retrospective and prospective studies of transmissible viral proventriculitis in broiler chickens in Brazil

et al. 2021

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We investigated the occurrence and pathologic findings of transmissible viral proventriculitis (TVP) associated with the chicken proventricular necrosis virus (CPNV) in commercial broiler chickens in southeastern Brazil. Seventy-three broilers, 25–36 d old, with a history of reduced growth, were referred to our veterinary pathology services from 2013 to 2017. Broilers were clinically examined, weighed, and euthanized for postmortem examination. Broilers of different ages with proventricular histologic lesions were positive for CPNV by RT-PCR; however, the intensity of histologic lesions was higher among 33-d-old animals, and viral RNA detection was more frequent among those that were 28 d old. In the proventriculi of 35 of 73 (48%) broilers, lesions were characterized by glandular epithelial necrosis, lymphoplasmacytic and histiocytic infiltrates, and metaplasia of glandular epithelium to ductal epithelium. In 24 of 73 (36%) broilers with histologic TVP-compatible lesions, CPNV was detected by RT-PCR for the viral protein 1 ( VP1) gene. Broilers with histologic lesions were lighter than expected compared to the Cobb 500 standard weight. TVP has not been reported previously in broiler chickens in Brazil, to our knowledge.

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Structural-functional changes in the proventriculus of poultry infected with transmissive viral proventriculitis

Kotsyumbas,

Zhyla,

Tyshkivska

et al. 2023

Regul. Mech. Biosyst.

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Transmissive viral proventriculitis (TVP) is a new avian disease, the etiology and pathogenesis and diagnostics of which remain unidentified, being therefore a subject of an active ongoing discussion among researchers and veterinary doctors. Cases of the disease have been reported and registered in many countries of the world. On poultry farms of Ukraine, this disease started to be diagnosed in 2019, according to the characteristic pathognomic changes. The article presents the results of pathoanatomical, histological and electronic-microscopical studies of the glandular part of the stomach of 62 ill broiler chickens aged 24-28 days and 7 clinical healthy broiler chickens of the same age. The samples of the proventriculus from the infected and clinically healthy chickens were fixated in 10% aqueous solution of neutral formalin and in 1.5% solution of glutaraldehyde in 0.2 molar cacodylate buffer (рН 7.2). Pathoanatomic necropsy of the infected broiler chickens revealed overall anemia, decrease in the volume of the spleen, thymus and fecal sac. In the glandular part of the stomach, the wall was slightly thickened, non-homogenously stained on the section, the mucous membrane being swollen, covered by dense whitish mucous. According to the histological study, the proventriculus of the broiler chickens had lymphocytic necrotizing proventriculitis. In the tubular-alveolar structures of the mucous membrane, there prevailed alteration of the secretory epithelium, with its transformation into ductal epithelium, combined with lymphocytic infiltration of the interstitium. Intensive necrosis of most glandular (oxynticopeptic) cells was accompanied by their exfoliation from the basal membrane of the secretory parts, with desquamated cells accumulating in enlarged lumens of alveoli and excretory ducts. Further, necrotized oxynticopeptic cells in the tubular-alveolar glands were actively replaced by cylindrical ductal cells, and also their hyperplasia and hypertrophy occurred. Diffusive and multifocal interalveolar round-cell infiltrates were mostly presented by lymphocytes, among which there occurred plasmocytes, macrophages, and heterophils. Ultrastructural studies of secretory cells revealed presence of pathogen virions in nucleus and cytoplasm. Based on the morphological changes in the birds’ proventriculus, we diagnosed transmissive viral proventriculitis, which had not been recorded in Ukraine before.

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Identification of Transmissible Viral Proventriculitis (TVP) in broiler chickens in Poland

Cited by 6 publications

References 4 publications

Chicken proventricular necrosis virus related transmissible viral proventriculitis in broiler chickens in Poland

Chicken proventricular necrosis virus related transmissible viral proventriculitis in broiler chickens in Poland

Retrospective and prospective studies of transmissible viral proventriculitis in broiler chickens in Brazil

Structural-functional changes in the proventriculus of poultry infected with transmissive viral proventriculitis

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