Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis

Karlsson, Niclas G.; Olson, Fredrik J.; Jovall, Per‐Åke; Andersch, Ylva; Enerbäck, Lennart; Hansson, Gunnar C.

doi:10.1042/bj3500805

Cited by 51 publications

(24 citation statements)

References 38 publications

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“…Mucosal scrapings from Sprague-Dawley rats were collected at different stages of the infection, and the insoluble mucin complex (5) was isolated after repeated extraction with guanidinium chloride. As described before, the amount of collected insoluble mucins increased up to day 12-13 and then quickly reverted to normal after parasite expulsion (18).…”

Section: The Effect Of the N Brasiliensis Infection On The Expressiosupporting

confidence: 73%

“…In a previous study we reported glycosylation alterations among sialylated oligosaccharides on the rat Muc2 mucin during N. brasiliensis infection (18). Detailed structural characterization of sialylated Muc2 oligosaccharides prepared from small intestinal mucosal scrapings of infected rats at different stages of the infection revealed two events of altered glycosylation.…”

mentioning

confidence: 78%

“…The N. brasiliensis infection is a model for studies of mucosal dynamics, because both quantitative and qualitative changes of the mucus occur during the infection. The detailed characterization of sialylated Muc2 oligosaccharides from N. brasiliensisinfected rats in a previous study revealed two events of an altered glycosylation (18), but it did not explain the reported increased HPA lectin reactivity in the small intestine of infected rats (16,17). When neutral mucin oligosaccharides were released and characterized, seven additional oligosaccharides were found in the infected animals, besides the normally expressed ones.…”

Section: Genbankmentioning

confidence: 89%

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Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection

Olson¹,

Johansson²,

Klinga-Levan³

et al. 2002

Journal of Biological Chemistry

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Neutral mucin oligosaccharides from the small intestine of control rats and rats infected with the parasite Nippostrongylus brasiliensis were released and analyzed by gas chromatography-mass spectrometry. Infected animals expressed seven blood group A-like structures that were all absent in the control animals. The blood group A nature of these epitopes was confirmed by blood group A reactivity of the prepared mucins, of which Muc2 was one. Transferase assays and Northern blotting on small intestines from infected animals showed that an ␣-N-acetylgalactosaminyltransferase similar to the human blood group A glycosyltransferase had been induced. The expression was a transient event, with a maximum at day 6 of the 13-daylong infection. The rat blood group A glycosyltransferase was cloned, revealing two forms with an amino acid similarity of 95%. Both types had blood group A transferase activity and were probably allelic because none of 12 analyzed inbred strains carried both types. The second type was found in outbred rats and in one inbred strain. First generation offspring of inbred rats of each type were heterozygous, further supporting the allelic hypothesis. The transient induction and the large allelic variation could suggest that glycosyltransferases are part of a dynamic system altering mucins and other glycoconjugates as a protecting mechanism against microbial challenges.The gastrointestinal tract is covered by a mucus layer, effectively protecting the epithelial cells from the hostile milieu including microorganisms and at the same time allowing digested nutrients and other smaller molecules to traverse. Microorganisms bind to intestinal mucus and epithelial cells; some of this binding is with high specificity to glycan epitopes (1). To maintain the protective layer despite continuous damaging processes, especially by these intestinal microorganisms, a system with a continuous mucus renewal is demanded. In the intestine, a majority of the mucus is produced by the goblet cells interspersed between the enterocytes. The matrix in the mucus is made up of gel-forming mucins. These are highly glycosylated proteins, where up to 80% of the mass is from O-linked oligosaccharides. Cloning and sequencing of mucin genes have revealed that the sites for glycosylation are located in domains of long stretches of protease-resistant sequences rich in the amino acids serine and threonine together with proline. Because these domains are typical for mucins, but also found in other proteins, they have been named mucin domains. Up until now, 13 human mucin genes have been identified and fully or partly sequenced (2-4), in addition to mucins identified from other species. The major intestinal mucin in rat is the Muc2 mucin, and the two mucin domains of this mucin have previously been isolated as two highly glycosylated peptides of 650 and 335 kDa, respectively (5, 6). The rat Muc2, as well as its human homologue MUC2, belongs to a class of gel forming mucins, gaining their viscous properties by oligomerization into large mucin...

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Section: The Effect Of the N Brasiliensis Infection On The Expressiosupporting

confidence: 73%

mentioning

confidence: 78%

Section: Genbankmentioning

confidence: 89%

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Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection

Olson¹,

Johansson²,

Klinga-Levan³

et al. 2002

Journal of Biological Chemistry

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“…spiralis , T. muris and H . polygyrus 76, 77, 78, 79. It has yet to be determined whether these changes occur as a result of the inflammatory environment or to resolve the infection; clearly more research is required to define the precise role of mucin glycans.…”

Section: Gi Nematode Infection and Alterations In Mucin Glycomementioning

confidence: 99%

A sticky end for gastrointestinal helminths; the role of the mucus barrier

Sharpe

Thornton

Grencis

2018

Parasite Immunology

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SummaryGastrointestinal (GI) nematodes are a group of successful multicellular parasites that have evolved to coexist within the intestinal niche of multiple species. It is estimated that over 10% of the world's population are chronically infected by GI nematodes, making this group of parasitic nematodes a major burden to global health. Despite the large number of affected individuals, there are few effective treatments to eradicate these infections. Research into GI nematode infections has primarily focused on defining the immunological and pathological consequences on host protection. One important but neglected aspect of host protection is mucus, and the concept that mucus is just a simple barrier is no longer tenable. In fact, mucus is a highly regulated and dynamic‐secreted matrix, underpinned by a physical hydrated network of highly glycosylated mucins, which is increasingly recognized to have a key protective role against GI nematode infections. Unravelling the complex interplay between mucins, the underlying epithelium and immune cells during infection are a major challenge and are required to fully define the protective role of the mucus barrier. This review summarizes the current state of knowledge on mucins and the mucus barrier during GI nematode infections, with particular focus on murine models of infection.

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“…Although the chemical alterations of colonic mucins in response to E. histolytica have not been addressed, changes in the glycosylation patterns of rat mucin have been observed during Nippostrongylus brasiliensis infection (77). These transient alterations in sialylated oligosaccharides have been directly related to parasite expulsion.…”

Section: Mucinmentioning

confidence: 99%

Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies

Guo

Houpt

Petri

2007

Current Opinion in Immunology

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The host-parasite relationship is based on a series of interplays between host defense mechanisms and parasite survival strategies. Progress has been made in understanding the role of host immune response in amebiasis. While host cells elaborate diverse mechanisms for pathogen expulsion, amebae have also developed complex strategies to modulate host immune response and facilitate their own survival. This paper will give an overview of current research on the mutual interactions between host and Entamoeba histolytica in human and experimental amebiasis. Understanding this crosstalk is crucial for the effective design and implementation of new vaccines and drugs for this leading parasitic disease.

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Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis

Cited by 51 publications

References 38 publications

Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection

Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection

A sticky end for gastrointestinal helminths; the role of the mucus barrier

Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies

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