Identification of transforming growth factor-β1 mRNA in virgin and pregnant rat uteri by in situ hybridization

Chen, Hualin; Yelavarthi, Krishna K.; Hunt, Joan S.

doi:10.1016/0165-0378(93)90065-p

Cited by 47 publications

(20 citation statements)

References 32 publications

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“…1). A recurrent observation of studies that characterized the expression of TGF-β in the endometrium during pregnancy was the identification of TGF-β1 or TGF-β2 at the fetal-maternal interface (Kauma et al, 1990;Tamada et al, 1990;Das et al, 1992;Chen et al, 1993;Selick et al, 1994;Lea et al 1995;Lennard et al, 1995). TGF-β in the extracellular matrix of decidual cells (Tamada et al, 1990;Graham et al, 1992) may provide a reservior of latent TGF-β that is activated by trophoblast-derived proteinases (Graham et al, 1992).…”

Section: Tgf-β Function In Endometrium During Pregnancymentioning

confidence: 99%

Transforming growth factor beta and the endometrium

Godkin

Doré²

1998

Reviews of Reproduction

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Section: Tgf-β Function In Endometrium During Pregnancymentioning

confidence: 99%

Transforming growth factor beta and the endometrium

Godkin

Doré²

1998

Reviews of Reproduction

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“…It is therefore thought to be involved in modulation of trophoblast invasion (Caniggia et al, 1999;Graham and Lala, 1992;Strickland and Richards, 1992). The three isoforms, TGF␤1, TGF␤2, and TGF␤3, have been identified at the human maternal interface (Ando et al, 1998;Chen et al, 1993;Graham and Lala, 1992;Vuckovic et al, 1992) by in situ hybridization and immunohistochemistry. A predominant expression of TGF␤1 in the villous tissue and of TGF␤2 in the decidual tissue has been observed (Altman et al, 1990;…”

Section: Characterization Of Human Trophoblastsmentioning

confidence: 99%

Characterization of Human Villous and Extravillous Trophoblasts Isolated from First Trimester Placenta

Tarrade

Kuen

Malassiné

et al. 2001

Laboratory Investigation

228

128

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SUMMARY:Trophoblasts of the human placenta differentiate along two pathways to give either extravillous cytotrophoblasts (EVCT) with invasive properties and that are implicated in the implantation process, or villous cytotrophoblasts (VCT) that by cell fusion form multinucleated syncytiotrophoblasts. We report the first isolation and purification of these two cell types from the same chorionic villi of first trimester human placenta. We also studied their differentiation in vitro. Electron microscopy showed that in contrast to VCT, EVCT had no microvilli but contained large fibrinoid inclusions. EVCT cultures required a matrix to invade, and as previously established, VCT cultured on plastic dishes aggregated and fused to form syncytiotrophoblasts. These differentiation processes were characterized by a particular pattern of gene expression as assessed by real-time PCR and confirmed by immunocytochemical analysis of the corresponding proteins. EVCT cultured in vitro expressed high levels of HLA-G, c-erbB2, human placental lactogen, and very little human chorionic gonadotropin. Interestingly, TGF␤2 was a marker of EVCT in vitro and in situ. These data offer a new tool for cell biologists to study the molecular mechanisms involved in human placental development and its pathology. (Lab Invest 2001, 81:1199 -1211.

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“…Human uNK cells express vascular endothelial growth factor A (VEGFA), VEGFC, PGF, angiopoietin 1 (ANGPT1), ANGPT2, matrix metalloproteinase 2 (MMP2), transforming growth factor beta 1 (TGFb1), and NKG5 (currently known as granulysin) (Langer et al 1999, Li et al 2001, Hanna et al 2006, Lash et al 2011a. Murine uNK cells express VEGFA, VEGFC, PGF, delta-like ligand 1 (DLL1), TGFb1, MMPs, tumor necrosis factor A, and inducible nitric oxide synthase (iNOS) (Chen et al 1993, Hunt 1994, Burnett & Hunt 2000, Wang et al 2000, Tayade et al 2007, Naruse et al 2009.…”

Section: Role Of Nk Cells In Normal Decidual Neoangiogenesismentioning

confidence: 99%

Uterine natural killer cells: supervisors of vasculature construction in early decidua basalis

et al. 2015

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Mammalian pregnancy involves tremendous de novo maternal vascular construction to adequately support conceptus development. In early mouse decidua basalis (DB), maternal uterine natural killer (uNK) cells oversee this process directing various aspects during the formation of supportive vascular networks. The uNK cells recruited to early implantation site DB secrete numerous factors that act in the construction of early decidual vessels (neoangiogenesis) as well as in the alteration of the structural components of newly developing and existing vessels (pruning and remodeling). Although decidual and placental development sufficient to support live births occur in the absence of normally functioning uNK cells, development and structure of implantation site are optimized through the presence of normally activated uNK cells. Human NK cells are also recruited to early decidua. Gestational complications including recurrent spontaneous abortion, fetal growth restriction, preeclampsia, and preterm labor are linked with the absence of human NK cell activation via paternally inherited conceptus transplantation antigens. This review summarizes the roles that mouse uNK cells normally play in decidual neoangiogenesis and spiral artery remodeling in mouse pregnancy and briefly discusses changes in early developmental angiogenesis due to placental growth factor deficiency.Reproduction (2015) 149 R91-R102

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Identification of transforming growth factor-β1 mRNA in virgin and pregnant rat uteri by in situ hybridization

Cited by 47 publications

References 32 publications

Transforming growth factor beta and the endometrium

Transforming growth factor beta and the endometrium

Characterization of Human Villous and Extravillous Trophoblasts Isolated from First Trimester Placenta

Uterine natural killer cells: supervisors of vasculature construction in early decidua basalis

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